CRISPLD2 (NM_031476) Human Tagged ORF Clone

CAT#: RC214912

CRISPLD2 (Myc-DDK-tagged)-Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2)

Product Images

Western blot validation of overexpression lysate (Cat# LY410482) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214912 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRISPLD2
• Synonyms: CRISP11; LCRISP2; LGL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214912 representing NM_031476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACC
  TCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGT
  CCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTG
  CAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGG
  CCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCA
  CTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
  CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACAC
  AGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGG
  AGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCC
  CCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGT
  GTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCAT
  TCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
  GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTA
  ACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAG
  CTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATC
  ACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACA
  AACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCA
  GCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
  CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCTG
  TGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGAC
  CTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTC
  CGGATCTTTGCTGTCAGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214912 representing NM_031476
  Red=Cloning site Green=Tags(s)
  
  MSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQV
  QPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTY
  PYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEA
  PYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSA
  VNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDI
  TRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDE
  PSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAF
  RIFAVRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031476
• ORF Size: 1491 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031476.1, NM_031476.2, NM_031476.3, NP_113664.1
• RefSeq Size: 4574 bp
• RefSeq ORF: 1494 bp
• Locus ID: 83716
• Domains: SCP, LCCL
• Protein Families: Secreted Protein, Transmembrane
• MW: 55.7 kDa