EHMT1/GLP (EHMT1) (NM_024757) Human Tagged ORF Clone

CAT#: RC214949

EHMT1 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403026) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214949 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EHMT1
• Synonyms: EHMT1-IT1; Eu-HMTase1; EUHMTASE1; FP13812; GLP; GLP1; KLEFS1; KMT1D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214949 representing NM_024757.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGCCGCCGATGCCGAGGCAGTTCCGGCGAGGGGGGAGCCTCAGCAGGATTGCTGTGTGAAAACC
  GAGCTGCTGGGAGAAGAGACACCTATGGCTGCCGATGAAGGCTCAGCAGAGAAACAGGCAGGAGAGGCC
  CACATGGCTGCGGACGGTGAGACCAATGGGTCTTGTGAAAACAGCGATGCCAGCAGTCATGCAAATGCT
  GCAAAGCACACTCAGGACAGCGCAAGGGTCAACCCCCAGGATGGCACCAACACACTAACTCGGATAGCG
  GAAAATGGGGTTTCAGAAAGAGACTCAGAAGCGGCGAAGCAAAACCACGTCACTGCCGACGACTTTGTG
  CAGACTTCTGTCATCGGCAGCAACGGATACATCTTAAATAAGCCGGCCCTACAGGCACAGCCCTTGAGG
  ACTACCAGCACTCTGGCCTCTTCGCTGCCTGGCCATGCTGCAAAAACCCTTCCTGGAGGGGCTGGCAAA
  GGCAGGACTCCAAGCGCTTTTCCCCAGACGCCAGCCGCCCCACCAGCCACCCTTGGGGAGGGGAGTGCT
  GACACAGAGGACAGGAAGCTCCCGGCCCCTGGCGCCGACGTCAAGGTCCACAGGGCACGCAAGACCATG
  CCGAAGTCCGTCGTGGGCCTGCATGCAGCCAGTAAAGATCCCAGAGAAGTTCGAGAAGCTAGAGATCAT
  AAGGAACCAAAAGAGGAGATCAACAAAAACATTTCTGACTTTGGACGACAGCAGCTTTTACCCCCCTTC
  CCATCCCTTCATCAGTCGCTACCTCAGAACCAGTGCTACATGGCCACCACAAAATCACAGACAGCTTGC
  TTGCCTTTTGTTTTAGCAGCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGCCTGGTT
  CCTAAGAAAAAGACCAAAGTATTAAAACAGAGGACGGTGATTGAGATGTTTAAGAGCATAACTCATTCC
  ACTGTGGGTTCCAAGGGGGAGAAGGACCTGGGCGCCAGCAGCCTGCACGTGAATGGGGAGAGCCTGGAG
  ATGGACTCGGATGAGGACGACTCAGAGGAGCTCGAGGAGGACGACGGCCATGGTGCAGAGCAGGCGGCC
  GCGTTCCCCACAGAGGACAGCAGGACTTCCAAGGAGAGCATGTCGGAGGCTGATCGCGCCCAGAAGATG
  GACGGGGAGTCCGAGGAGGAGCAGGAGTCCGTGGACACCGGGGAGGAGGAGGAAGGCGGTGACGAGTCT
  GACCTGAGTTCGGAATCCAGCATTAAGAAGAAATTTCTCAAGAGGAAAGGAAAGACCGACAGTCCCTGG
  ATCAAGCCAGCCAGGAAAAGGAGGCGGAGAAGTAGAAAGAAGCCCAGCGGTGCCCTCGGTTCTGAGTCG
  TATAAGTCATCTGCAGGAAGCGCTGAGCAGACGGCACCAGGAGACAGCACAGGGTACATGGAAGTTTCT
  CTGGACTCCCTGGATCTCCGAGTCAAAGGAATTCTGTCTTCACAAGCAGAAGGGTTGGCCAACGGTCCA
  GATGTGCTGGAGACAGACGGCCTCCAGGAAGTGCCTCTCTGCAGCTGCCGGATGGAAACACCGAAGAGT
  CGAGAGATCACCACACTGGCCAACAACCAGTGCATGGCTACAGAGAGCGTGGACCATGAATTGGGCCGG
  TGCACAAACAGCGTGGTCAAGTATGAGCTGATGCGCCCCTCCAACAAGGCCCCGCTCCTCGTGCTGTGT
  GAAGACCACCGGGGCCGCATGGTGAAGCACCAGTGCTGTCCTGGCTGTGGCTACTTCTGCACAGCGGGT
  AATTTTATGGAGTGTCAGCCCGAGAGCAGCATCTCTCACCGTTTCCACAAAGACTGTGCCTCTCGAGTC
  AATAACGCCAGCTATTGTCCCCACTGTGGGGAGGAGAGCTCCAAGGCCAAAGAGGTGACGATAGCTAAA
  GCAGACACCACCTCGACCGTGACACCAGTCCCCGGGCAGGAGAAGGGCTCGGCCCTGGAGGGCAGGGCC
  GACACCACAACGGGCAGTGCTGCCGGGCCACCACTCTCGGAGGACGACAAGCTGCAGGGTGCAGCCTCC
  CACGTGCCCGAGGGCTTTGATCCAACGGGACCTGCTGGGCTTGGGAGGCCAACTCCCGGCCTTTCCCAG
  GGACCAGGGAAGGAAACCTTGGAGAGCGCTCTCATCGCCCTCGACTCGGAAAAACCCAAGAAGCTTCGC
  TTCCACCCAAAGCAGCTGTACTTCTCCGCCAGGCAAGGGGAGCTTCAGAAGGTGCTCCTCATGCTGGTG
  GACGGAATTGACCCCAACTTCAAAATGGAGCACCAGAATAAGCGCTCTCCACTGCACGCCGCGGCAGAG
  GCTGGACACGTGGACATCTGCCACATGCTGGTTCAGGCGGGCGCTAATATTGACACCTGCTCAGAAGAC
  CAGAGGACCCCGTTGATGGAAGCAGCCGAAAACAACCATCTGGAAGCAGTGAAGTACCTCATCAAGGCT
  GGGGCCCTGGTGGATCCCAAGGACGCAGAGGGCTCTACGTGTTTGCACCTGGCTGCCAAGAAAGGCCAC
  TACGAAGTGGTCCAGTACCTGCTTTCAAATGGACAGATGGACGTCAACTGTCAGGATGACGGAGGCTGG
  ACACCCATGATCTGGGCCACAGAGTACAAGCACGTGGACCTCGTGAAGCTGCTGCTGTCCAAGGGCTCT
  GACATCAACATCCGAGACAACGAGGAGAACATTTGCCTGCACTGGGCGGCGTTCTCCGGCTGCGTGGAC
  ATAGCCGAGATCCTGCTGGCTGCCAAGTGCGACCTCCACGCCGTGAACATCCACGGAGACTCGCCACTG
  CACATTGCCGCCCGGGAGAACCGCTACGACTGTGTCGTCCTCTTTCTTTCTCGGGATTCAGATGTCACC
  TTAAAGAACAAGGAAGGAGAGACGCCCCTGCAGTGTGCGAGCCTCAACTCTCAGGTGTGGAGCGCTCTG
  CAGATGAGCAAGGCTCTGCAGGACTCGGCCCCCGACAGGCCCAGCCCCGTGGAGAGGATAGTGAGCAGG
  GACATCGCTCGAGGCTACGAGCGCATCCCCATCCCCTGTGTCAACGCCGTGGACAGCGAGCCATGCCCC
  AGCAACTACAAGTACGTCTCTCAGAACTGCGTGACGTCCCCCATGAACATCGACAGAAATATCACTCAT
  CTGCAGTACTGCGTGTGCATCGACGACTGCTCCTCCAGCAACTGCATGTGCGGCCAGCTCAGCATGCGC
  TGCTGGTACGACAAGGATGGCCGGCTCCTGCCAGAGTTCAACATGGCGGAGCCTCCCTTGATCTTCGAA
  TGCAACCACGCGTGCTCCTGCTGGAGGAACTGCCGAAATCGCGTCGTACAGAATGGTCTCAGGGCAAGG
  CTGCAGCTCTACCGGACGCGGGACATGGGCTGGGGCGTGCGGTCCCTGCAGGACATCCCACCAGGCACC
  TTTGTCTGCGAGTATGTTGGGGAGCTGATTTCAGACTCAGAAGCCGACGTTCGAGAGGAAGATTCTTAC
  CTCTTTGATCTCGACAATAAGGACGGGGAGGTTTACTGCATCGACGCGCGGTTCTACGGGAACGTCAGC
  CGGTTCATCAACCACCACTGCGAGCCCAACCTGGTGCCCGTGCGCGTGTTCATGGCCCACCAGGACCTG
  CGGTTCCCCCGGATCGCCTTCTTCAGCACCCGCCTGATCGAGGCCGGCGAGCAGCTCGGGTTTGACTAT
  GGAGAGCGCTTCTGGGACATCAAAGGCAAGCTCTTCAGCTGCCGCTGCGGCTCCCCCAAGTGCCGGCAC
  TCGAGCGCGGCCCTGGCCCAGCGTCAGGCCAGCGCGGCCCAGGAGGCCCAGGAGGACGGCTTGCCCGAC
  ACCAGCTCCGCGGCTGCCGCCGACCCCCTA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC214949
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAAADAEAVPARGEPQQDCCVKTELLGEETPMAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANA
  AKHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPALQAQPLR
  TTSTLASSLPGHAAKTLPGGAGKGRTPSAFPQTPAAPPATLGEGSADTEDRKLPAPGADVKVHRARKTM
  PKSVVGLHAASKDPREVREARDHKEPKEEINKNISDFGRQQLLPPFPSLHQSLPQNQCYMATTKSQTAC
  LPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGSKGEKDLGASSLHVNGESLE
  MDSDEDDSEELEEDDGHGAEQAAAFPTEDSRTSKESMSEADRAQKMDGESEEEQESVDTGEEEEGGDES
  DLSSESSIKKKFLKRKGKTDSPWIKPARKRRRRSRKKPSGALGSESYKSSAGSAEQTAPGDSTGYMEVS
  LDSLDLRVKGILSSQAEGLANGPDVLETDGLQEVPLCSCRMETPKSREITTLANNQCMATESVDHELGR
  CTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRV
  NNASYCPHCGEESSKAKEVTIAKADTTSTVTPVPGQEKGSALEGRADTTTGSAAGPPLSEDDKLQGAAS
  HVPEGFDPTGPAGLGRPTPGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLV
  DGIDPNFKMEHQNKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLEAVKYLIKA
  GALVDPKDAEGSTCLHLAAKKGHYEVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGS
  DINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVT
  LKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCP
  SNYKYVSQNCVTSPMNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFE
  CNHACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIPPGTFVCEYVGELISDSEADVREEDSY
  LFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMAHQDLRFPRIAFFSTRLIEAGEQLGFDY
  GERFWDIKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQEDGLPDTSSAAAADPL
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024757
• ORF Size: 3894 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024757.5
• RefSeq Size: 5123 bp
• RefSeq ORF: 3897 bp
• Locus ID: 79813
• UniProt ID: Q9H9B1
• Cytogenetics: 9q34.3
• Domains: SET, ANK, PreSET, Pre-SET
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 141.5 kDa
• Gene Summary: The protein encoded by this gene is a histone methyltransferase that methylates the lysine-9 position of histone H3. This action marks the genomic region packaged with these methylated histones for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]