Protein Kinase D2 (PRKD2) (NM_001079882) Human Tagged ORF Clone

CAT#: RC214956

PRKD2 (Myc-DDK-tagged)-Human protein kinase D2 (PRKD2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKD2
• Synonyms: HSPC187; nPKC-D2; PKD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214956 representing NM_001079882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTCGGCCTAGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAACTACCACAAGCGCTGTG
  CCTTCAGCATCCCCAACAACTGTAGTGGGGCCCGCAAACGGCGCCTGTCATCCACGTCTCTGGCCAGTGG
  CCACTCGGTGCGCCTCGGCACCTCCGAGTCCCTGCCCTGCACGGCTGAAGAGCTGAGCCGTAGCACCACC
  GAACTCCTGCCTCGCCGTCCCCCGTCATCCTCTTCCTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCA
  TTGAGCTGGACAAGATGCTGCTCTCCAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACG
  GCCCACCGTTTGCCAGGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGAC
  TGCAAGTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAGGCCCTTATCA
  ATGGAGATGTGCCGATGGAGGAGGCCACCGATTTCAGCGAGGCTGACAAGAGCGCCCTCATGGATGAGTC
  AGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAATGCGCTCCACGCCAGTGAGGAGGAGGAAGGC
  GAGGGAGGCAAGGCCCAGAGCTCCCTGGGGTACATCCCCCTAATGAGGGTGGTGCAATCGGTGCGACACA
  CGACGCGGAAATCCAGCACCACGCTGCGGGAGGGTTGGGTGGTTCATTACAGCAACAAGGACACGCTGAG
  AAAGCGGCACTATTGGCGCCTGGACTGCAAGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATAC
  TATAAGGAAATTCCGCTGTCAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTTGTGCCGCCGG
  GCACCAACCCACACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTCGTGGGCGAGATGCCTGGCGG
  GACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCCCGGGGCTGGGAGACAGCCATCCGCCAGGCC
  CTGATGCCCGTCATCCTTCAGGACGCACCCAGCGCCCCAGGCCACGCGCCCCACAGACAAGCTTCTCTGA
  GCATCTCTGTGTCCAACAGTCAGATCCAAGAGAATGTGGACATTGCCACTGTCTACCAGATCTTCCCTGA
  CGAAGTGCTGGGCTCAGGGCAGTTTGGAGTGGTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTG
  GCAGTTAAGGTCATTGACAAACTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAATGAAGTGGCCA
  TTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTCGAGACGCCTGAGAAAGTGTT
  TGTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATGATCCTGTCCAGTGAGAAGGGCCGGCTGCCT
  GAGCGCCTCACCAAGTTCCTCATCACCCAGATCCTGGTGGCTTTGAGACACCTTCACTTCAAGAACATTG
  TCCACTGTGACTTGAAACCAGAAAACGTGTTGCTGGCATCAGCAGACCCATTTCCTCAGGTGAAGCTGTG
  TGACTTTGGCTTTGCTCGCATCATCGGCGAGAAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTAC
  CTGGCACCCGAGGTGCTGCTCAACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTGGGCGTGATCA
  TGTACGTCAGCCTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATCAATGACCAGATCCAGAACGC
  CGCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCAGCTGGAGCCATTGACCTCATCAACAACCTG
  CTGCAGGTGAAGATGCGCAAACGCTACAGCGTGGACAAATCTCTCAGCCACCCCTGGTTACAGGAGTACC
  AGACGTGGCTGGACCTCCGAGAGCTGGAGGGGAAGATGGGAGAGCGATACATCACGCATGAGAGTGACGA
  CGCGCGCTGGGAGCAGTTTGCAGCAGAGCATCCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTC
  GGTGGGGCCTGTCCACCACAGGACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214956 representing NM_001079882
  Red=Cloning site Green=Tags(s)
  
  MLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTT
  ELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKD
  CKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEG
  EGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRY
  YKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQA
  LMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDV
  AVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLP
  ERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAY
  LAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNL
  LQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDL
  GGACPPQDHDMQGLAERISVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079882
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001079882.1, NP_001073351.1
• RefSeq Size: 2939
• RefSeq ORF: 2166
• Locus ID: 25865
• Protein Families: Druggable Genome, Protein Kinase
• MW: 79.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]