Protein Kinase D2 (PRKD2) (NM_001079882) Human Untagged Clone

CAT#: SC323487

PRKD2 (untagged)-Kinase deficient mutant (K580M) of Human protein kinase D2 (PRKD2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKD2
• Synonyms: HSPC187; nPKC-D2; PKD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323487 sequence for NM_001079882 edited (data generated by NextGen Sequencing)
  ATGCTCTTCGGCCTAGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAACTACCAC
  AAGCGCTGTGCCTTCAGCATCCCCAACAACTGTAGTGGGGCCCGCAAACGGCGCCTGTCA
  TCCACGTCTCTGGCCAGTGGCCACTCGGTGCGCCTCGGCACCTCCGAGTCCCTGCCCTGC
  ACGGCTGAAGAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCC
  TCTTCCTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTG
  CTCTCCAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTT
  TGCCAGGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGAC
  TGCAAGTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAG
  GCCCTTATCAATGGAGATGTGCCGATGGAGGAGGCCACCGATTTCAGCGAGGCTGACAAG
  AGCGCCCTCATGGATGAGTCAGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAAT
  GCGCTCCACGCCAGTGAGGAGGAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGG
  TACATCCCCCTAATGAGGGTGGTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACC
  ACGCTGCGGGAGGGTTGGGTGGTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCAC
  TATTGGCGCCTGGACTGCAAGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATAC
  TATAAGGAAATTCCGCTGTCAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTT
  GTGCCGCCGGGCACCAACCCACACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTC
  GTGGGCGAGATGCCTGGCGGGACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCC
  CGGGGCTGGGAGACAGCCATCCGCCAGGCCCTGATGCCCGTCATCCTTCAGGACGCACCC
  AGCGCCCCAGGCCACGCGCCCCACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAACAGT
  CAGATCCAAGAGAATGTGGACATTGCCACTGTCTACCAGATCTTCCCTGACGAAGTGCTG
  GGCTCAGGGCAGTTTGGAGTGGTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTG
  GCAGTTAAGGTCATTGACAAACTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAAT
  GAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTC
  GAGACGCCTGAGAAAGTGTTTGTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATG
  ATCCTGTCCAGTGAGAAGGGCCGGCTGCCTGAGCGCCTCACCAAGTTCCTCATCACCCAG
  ATCCTGGTGGCTTTGAGACACCTTCACTTCAAGAACATTGTCCACTGTGACTTGAAACCA
  GAAAACGTGTTGCTGGCATCAGCAGACCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGC
  TTTGCTCGCATCATCGGCGAGAAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTAC
  CTGGCACCCGAGGTGCTGCTCAACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTG
  GGCGTGATCATGTACGTCAGCCTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATC
  AATGACCAGATCCAGAACGCCGCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCA
  GCTGGAGCCATTGACCTCATCAACAACCTGCTGCAGGTGAAGATGCGCAAACGCTACAGC
  GTGGACAAATCTCTCAGCCACCCCTGGTTACAGGAGTACCAGACGTGGCTGGACCTCCGA
  GAGCTGGAGGGGAAGATGGGAGAGCGATACATCACGCATGAGAGTGACGACGCGCGCTGG
  GAGCAGTTTGCAGCAGAGCATCCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTC
  GGTGGGGCCTGTCCACCACAGGACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTT
  CTCTGA
  
  Clone variation with respect to NM_001079882.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001079882 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTG
  ACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCCACCCCAGCT
  CCCCCTTCCAGCCACCAAACCCACGCCGGCGCCCCCTCCCCGTACAATTGGGGCGCTGGCATCCTGCCCG
  GCTCGCGCTGGGGTTGGGAGGGGGCAGGCAGGAAGCGAGGGCCTGCGGGGTCTCTGCGTTTCCGGGGGAA
  ACAGCCGGCCCTGCCCTGGGAGGGTCACAGTCCGCCCGCTGCTGAAGGCGGGCTCTGGAGGCTTTTCCGT
  CGCCACATCCCCTCTCCCCGCCCCCTTCAGTTTCCCTGGAGTTGTGGGCTTTCTTACGGGCCTTAACAAC
  AAGAATCCTGCCTTTTTCAAAACATGGACCCCACCTTCGGCAAACCTCTGCAACCTTGTGTGCCCCTCGT
  CCGGAGCATTCCAGAAGGGCACCCTGTTGAAGTTGTTGCTGTCGGCCTGGCCCACTTTCAGGAACTTCCA
  AACCCCCCCCCCCCCCCAGGGGCACTCATTCGGGGCGCGTGCTTTGTGGATCTGGGGGGAAAACCCTTTT
  GGCCCTAGGGCCCGGGCCCAAGGGCAGGGGCGGGCCTGAAACACAAACGCTGCCTCTTCACACCAAACTG
  TGTGGGGCCCCCACACGGCCCGGTCACATTCTGGAGCGGGGCAATTGTCCCTGCGACTCAGTACTCCCGC
  GCGGAGTGTAATAGAGATATAACAGCGCTTCCGCGGCGCGC
  
  Kinase domain sequence

  >SC323487 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AGCMTTKACACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGGCAGTTTGGAGTGGTCTATG
  GAGGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTATGGTCATTGACAAACTGCGCTTCCCTACCAA
  GCAGGAGAGCCAGCTCCGGAATGAAGTGGCCATTCTGCAGAGCCTGCGGCATCCCGGGATCGTGAACCTG
  GAGTGCATGTTCGAGACGCCTGAGAAAGTGTTTGTGGTGATGGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001079882
• ORF Size: 2166 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_001079882.1, NP_001073351.1
• RefSeq Size: 2939
• RefSeq ORF: 2166
• Locus ID: 25865
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (B) has a shorter N-terminus when compared to isoform A.