CACNA1A (NM_023035) Human Tagged ORF Clone

CAT#: RC214990

CACNA1A (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (CACNA1A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1A
• Synonyms: APCA; BI; CACNL1A4; CAV2.1; DEE42; EA2; EIEE42; FHM; HPCA; MHP; MHP1; SCA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214990 representing NM_023035.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCCGCTTCGGAGACGAGATGCCGGCCCGCTACGGGGGAGGAGGCTCCGGGGCAGCCGCCGGGGTG
  GTCGTGGGCAGCGGAGGCGGGCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGGGCGCAAAGG
  ATGTACAAGCAGTCAATGGCGCAGAGAGCGCGGACCATGGCACTCTACAACCCCATCCCCGTCCGACAG
  AACTGCCTCACGGTTAACCGGTCTCTCTTCCTCTTCAGCGAAGACAACGTGGTGAGAAAATACGCCAAA
  AAGATCACCGAATGGCCTCCCTTTGAATATATGATTTTAGCCACCATCATAGCGAATTGCATCGTCCTC
  GCACTGGAGCAGCATCTGCCTGATGATGACAAGACCCCGATGTCTGAACGGCTGGATGACACAGAACCA
  TACTTCATTGGAATTTTTTGTTTCGAGGCTGGAATTAAAATCATTGCCCTTGGGTTTGCCTTCCACAAA
  GGCTCCTACTTGAGGAATGGCTGGAATGTCATGGACTTTGTGGTGGTGCTAACGGGCATCTTGGCGACA
  GTTGGGACGGAGTTTGACCTACGGACGCTGAGGGCAGTTCGAGTGCTGCGGCCGCTCAAGCTGGTGTCT
  GGAATCCCAAGTTTACAAGTCGTCCTGAAGTCGATCATGAAGGCGATGATCCCTTTGCTGCAGATCGGC
  CTCCTCCTATTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGAAAATTTCAT
  ACCACCTGCTTTGAAGAGGGGACAGATGACATTCAGGGTGAGTCTCCGGCTCCATGTGGGACAGAAGAG
  CCCGCCCGCACCTGCCCCAATGGGACCAAATGTCAGCCCTACTGGGAAGGGCCCAACAACGGGATCACT
  CAGTTCGACAACATCCTGTTTGCAGTGCTGACTGTTTTCCAGTGCATAACCATGGAAGGGTGGACTGAT
  CTCCTCTACAATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATC
  ATCGGCTCCTTTTTTATGCTGAACCTTGTGCTGGGTGTGCTGTCAGGGGAGTTTGCCAAAGAAAGGGAA
  CGGGTGGAGAACCGGCGGGCTTTTCTGAAGCTGAGGCGGCAACAACAGATTGAACGTGAGCTCAATGGG
  TACATGGAGTGGATCTCAAAAGCAGAAGAGGTGATCCTCGCCGAGGATGAAACTGACGGGGAGCAGAGG
  CATCCCTTTGATGGAGCTCTGCGGAGAACCACCATAAAGAAAAGCAAGACAGATTTGCTCAACCCCGAA
  GAGGCTGAGGATCAGCTGGCTGATATAGCCTCTGTGGGTTCTCCCTTCGCCCGAGCCAGCATTAAAAGT
  GCCAAGCTGGAGAACTCGACCTTTTTTCACAAAAAGGAGAGGAGGATGCGTTTCTACATCCGCCGCATG
  GTCAAAACTCAGGCCTTCTACTGGACTGTACTCAGTTTGGTAGCTCTCAACACGCTGTGTGTTGCTATT
  GTTCACTACAACCAGCCCGAGTGGCTCTCCGACTTCCTTTACTATGCAGAATTCATTTTCTTAGGACTC
  TTTATGTCCGAAATGTTTATAAAAATGTACGGGCTTGGGACGCGGCCTTACTTCCACTCTTCCTTCAAC
  TGCTTTGACTGTGGGGTTATCATTGGGAGCATCTTCGAGGTCATCTGGGCTGTCATAAAACCTGGCACA
  TCCTTTGGAATCAGCGTGTTACGAGCCCTCAGGTTATTGCGTATTTTCAAAGTCACAAAGTACTGGGCA
  TCTCTCAGAAACCTGGTCGTCTCTCTCCTCAACTCCATGAAGTCCATCATCAGCCTGTTGTTTCTCCTT
  TTCCTGTTCATTGTCGTCTTCGCCCTTTTGGGAATGCAACTCTTCGGCGGCCAGTTTAATTTCGATGAA
  GGGACTCCTCCCACCAACTTCGATACTTTTCCAGCAGCAATAATGACGGTGTTTCAGATCCTGACGGGC
  GAAGACTGGAACGAGGTCATGTACGACGGGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTC
  TCCATCTATTTCATTGTACTGACGCTCTTTGGGAACTACACCCTCCTGAATGTGTTCTTGGCCATCGCT
  GTGGACAATCTGGCCAACGCCCAGGAGCTCACCAAGGTGGAGGCGGACGAGCAAGAGGAAGAAGAAGCA
  GCGAACCAGAAACTTGCCCTACAGAAAGCCAAGGAGGTGGCAGAAGTGAGTCCTCTGTCCGCGGCCAAC
  ATGTCTATAGCTGTGAAAGAGCAACAGAAGAATCAAAAGCCAGCCAAGTCCGTGTGGGAGCAGCGGACC
  AGTGAGATGCGAAAGCAGAACTTGCTGGCCAGCCGGGAGGCCCTGTATAACGAAATGGACCCGGACGAG
  CGCTGGAAGGCTGCCTACACGCGGCACCTGCGGCCAGACATGAAGACGCACTTGGACCGGCCGCTGGTG
  GTGGACCCGCAGGAGAACCGCAACAACAACACCAACAAGAGCCGGGCGGCCGAGCCCACCGTGGACCAG
  CGCCTCGGCCAGCAGCGCGCCGAGGACTTCCTCAGGAAACAGGCCCGCTACCACGATCGGGCCCGGGAC
  CCCAGCGGCTCGGCGGGCCTGGACGCACGGAGGCCCTGGGCGGGAAGCCAGGAGGCCGAGCTGAGCCGG
  GAGGGACCCTACGGCCGCGAGTCGGACCACCACGCCCGGGAGGGCAGCCTGGAGCAACCCGGGTTCTGG
  GAGGGCGAGGCCGAGCGAGGCAAGGCCGGGGACCCCCACCGGAGGCACGTGCACCGGCAGGGGGGCAGC
  AGGGAGAGCCGCAGCGGGTCCCCGCGCACGGGCGCGGACGGGGAGCATCGACGTCATCGCGCGCACCGC
  AGGCCCGGGGAGGAGGGTCCGGAGGACAAGGCGGAGCGGAGGGCGCGGCACCGCGAGGGCAGCCGGCCG
  GCCCGGGGCGGCGAGGGCGAGGGCGAGGGCCCCGACGGGGGCGAGCGCAGGAGAAGGCACCGGCATGGC
  GCTCCAGCCACGTACGAGGGGGACGCGCGGAGGGAGGACAAGGAGCGGAGGCATCGGAGGAGGAAAGAG
  AACCAGGGCTCCGGGGTCCCTGTGTCGGGCCCCAACCTGTCAACCACCCGGCCAATCCAGCAGGACCTG
  GGCCGCCAAGACCCACCCCTGGCAGAGGATATTGACAACATGAAGAACAACAAGCTGGCCACCGCGGAG
  TCGGCCGCTCCCCACGGCAGCCTTGGCCACGCCGGCCTGCCCCAGAGCCCAGCCAAGATGGGAAACAGC
  ACCGACCCCGGCCCCATGCTGGCCATCCCTGCCATGGCCACCAACCCCCAGAACGCCGCCAGCCGCCGG
  ACGCCCAACAACCCGGGGAACCCATCCAATCCCGGCCCCCCCAAGACCCCCGAGAATAGCCTTATCGTC
  ACCAACCCCAGCGGCACCCAGACCAATTCAGCTAAGACTGCCAGGAAACCCGACCACACCACAGTGGAC
  ATCCCCCCAGCCTGCCCACCCCCCCTCAACCACACCGTCGTACAAGTGAACAAAAACGCCAACCCAGAC
  CCACTGCCAAAAAAAGAGGAAGAGAAGAAGGAGGAGGAGGAAGACGACCGTGGGGAAGACGGCCCTAAG
  CCAATGCCTCCCTATAGCTCCATGTTCATCCTGTCCACGACCAACCCCCTTCGCCGCCTGTGCCATTAC
  ATCCTGAACCTGCGCTACTTTGAGATGTGCATCCTCATGGTCATTGCCATGAGCAGCATCGCCCTGGCC
  GCCGAGGACCCTGTGCAGCCCAACGCACCTCGGAACAACGTGCTGCGATACTTTGACTACGTTTTTACA
  GGCGTCTTTACCTTTGAGATGGTGATCAAGATGATTGACCTGGGGCTCGTCCTGCATCAGGGTGCCTAC
  TTCCGTGACCTCTGGAATATTCTCGACTTCATAGTGGTCAGTGGGGCCCTGGTAGCCTTTGCCTTCACT
  GGCAATAGCAAAGGAAAAGACATCAACACGATTAAATCCCTCCGAGTCCTCCGGGTGCTACGACCTCTT
  AAAACCATCAAGCGGCTGCCAAAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCACTTAAAAACGTC
  TTCAACATCCTCATCGTCTACATGCTATTCATGTTCATCTTCGCCGTGGTGGCTGTGCAGCTCTTCAAG
  GGGAAATTCTTCCACTGCACTGACGAGTCCAAAGAGTTTGAGAAAGATTGTCGAGGCAAATACCTCCTC
  TACGAGAAGAATGAGGTGAAGGCGCGAGACCGGGAGTGGAAGAAGTATGAATTCCATTACGACAATGTG
  CTGTGGGCTCTGCTGACCCTCTTCACCGTGTCCACGGGAGAAGGCTGGCCACAGGTCCTCAAGCATTCG
  GTGGACGCCACCTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATTTTCTACGTC
  GTCTACTTTGTGGTGTTCCCCTTCTTCTTTGTCAATATCTTTGTGGCCTTGATCATCATCACCTTCCAG
  GAGCAAGGGGACAAGATGATGGAGGAATACAGCCTGGAGAAAAATGAGAGGGCCTGCATTGATTTCGCC
  ATCAGCGCCAAGCCGCTGACCCGACACATGCCGCAGAACAAGCAGAGCTTCCAGTACCGCATGTGGCAG
  TTCGTGGTGTCTCCGCCTTTCGAGTACACGATCATGGCCATGATCGCCCTCAACACCATCGTGCTTATG
  ATGAAGTTCTATGGGGCTTCTGTTGCTTATGAAAATGCCCTGCGGGTGTTCAACATCGTCTTCACCTCC
  CTCTTCTCTCTGGAATGTGTGCTGAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGG
  AACATCTTCGACTTTGTGACTGTTCTGGGCAGCATCACCGATATCCTCGTGACTGAGTTTGGGAATCCG
  AATAACTTCATCAACCTGAGCTTTCTCCGCCTCTTCCGAGCTGCCCGGCTCATCAAACTTCTCCGTCAG
  GGTTACACCATCCGCATTCTTCTCTGGACCTTTGTGCAGTCCTTCAAGGCCCTGCCTTATGTCTGTCTG
  CTGATCGCCATGCTCTTCTTCATCTATGCCATCATTGGGATGCAGGTGTTTGGTAACATTGGCATCGAC
  GTGGAGGACGAGGACAGTGATGAAGATGAGTTCCAAATCACTGAGCACAATAACTTCCGGACCTTCTTC
  CAGGCCCTCATGCTTCTCTTCCGGAGTGCCACCGGGGAAGCTTGGCACAACATCATGCTTTCCTGCCTC
  AGCGGGAAACCGTGTGATAAGAACTCTGGCATCCTGACTCGAGAGTGTGGCAATGAATTTGCTTATTTT
  TACTTTGTTTCCTTCATCTTCCTCTGCTCGTTTCTGATGCTGAATCTCTTTGTCGCCGTCATCATGGAC
  AACTTTGAGTACCTCACCCGAGACTCCTCCATCCTGGGCCCCCACCACCTGGATGAGTACGTGCGTGTC
  TGGGCCGAGTATGACCCCGCAGCTTGGGGCCGCATGCCTTACCTGGACATGTATCAGATGCTGAGACAC
  ATGTCTCCGCCCCTGGGTCTGGGGAAGAAGTGTCCGGCCAGAGTGGCTTACAAGCGGCTTCTGCGGATG
  GACCTGCCCGTCGCAGATGACAACACCGTCCACTTCAATTCCACCCTCATGGCTCTGATCCGCACAGCC
  CTGGACATCAAGATTGCCAAGGGAGGAGCCGACAAACAGCAGATGGACGCTGAGCTGCGGAAGGAGATG
  ATGGCGATTTGGCCCAATCTGTCCCAGAAGACGCTAGACCTGCTGGTCACACCTCACAAGTCCACGGAC
  CTCACCGTGGGGAAGATCTACGCAGCCATGATGATCATGGAGTACTACCGGCAGAGCAAGGCCAAGAAG
  CTGCAGGCCATGCGCGAGGAGCAGGACCGGACACCCCTCATGTTCCAGCGCATGGAGCCCCCGTCCCCA
  ACGCAGGAAGGGGGACCTGGCCAGAACGCCCTCCCCTCCACCCAGCTGGACCCAGGAGGAGCCCTGATG
  GCTCACGAAAGCGGCCTCAAGGAGAGCCCGTCCTGGGTGACCCAGCGTGCCCAGGAGATGTTCCAGAAG
  ACGGGCACATGGAGTCCGGAACAAGGCCCCCCTACCGACATGCCCAACAGCCAGCCTAACTCTCAGTCC
  GTGGAGATGCGAGAGATGGGCAGAGATGGCTACTCCGACAGCGAGCACTACCTCCCCATGGAAGGCCAG
  GGCCGGGCTGCCTCCATGCCCCGCCTCCCTGCAGAGAACCAGAGGAGAAGGGGCCGGCCACGTGGGAAT
  AACCTCAGTACCATCTCAGACACCAGCCCCATGAAGCGTTCAGCCTCCGTGCTGGGCCCCAAGGCCCGA
  CGCCTGGACGATTACTCGCTGGAGCGGGTCCCGCCCGAGGAGAACCAGCGGCACCACCAGCGGCGCCGC
  GACCGCAGCCACCGCGCCTCTGAGCGCTCCCTGGGCCGCTACACCGATGTGGACACAGGCTTGGGGACA
  GACCTGAGCATGACCACCCAATCCGGGGACCTGCCGTCGAAGGAGCGGGACCAGGAGCGGGGCCGGCCC
  AAGGATCGGAAGCATCGACAGCACCACCACCACCACCACCACCACCACCATCCCCCGCCCCCCGACAAG
  GACCGCTATGCCCAGGAACGGCCGGACCACGGCCGGGCACGGGCTCGGGACCAGCGCTGGTCCCGCTCG
  CCCAGCGAGGGCCGAGAGCACATGGCGCACCGGCAGGGCAGTAGTTCCGTAAGTGGAAGCCCAGCCCCC
  TCAACATCTGGTACCAGCACTCCGCGGCGGGGCCGCCGCCAGCTCCCCCAGACCCCCTCCACCCCCCGG
  CCACACGTGTCCTATTCCCCTGTGATCCGTAAGGCCGGCGGCTCGGGGCCCCCGCAGCAGCAGCAGCAG
  CAGCAGCAGCAGCAGCAGCAGCAGGCGGTGGCCAGGCCGGGCCGGGCGGCCACCAGCGGCCCTCGGAGG
  TACCCAGGCCCCACGGCCGAGCCTCTGGCCGGAGATCGGCCGCCCACGGGGGGCCACAGCAGCGGCCGC
  TCGCCCAGGATGGAGAGGCGGGTCCCAGGCCCGGCCCGGAGCGAGTCCCCCAGGGCCTGTCGACACGGC
  GGGGCCCGGTGGCCGGCATCTGGCCCGCACGTGTCCGAGGGGCCCCCGGGTCCCCGGCACCATGGCTAC
  TACCGGGGCTCCGACTACGACGAGGCCGATGGCCCGGGCAGCGGGGGCGGCGAGGAGGCCATGGCCGGG
  GCCTACGACGCGCCACCCCCCGTACGACACGCGTCCTCGGGCGCCACCGGGCGCTCGCCCAGGACTCCC
  CGGGCCTCGGGCCCGGCCTGCGCCTCGCCTTCTCGGCACGGCCGGCGACTCCCCAACGGCTACTACCCG
  GCGCACGGACTGGCCAGGCCCCGCGGGCCGGGCTCCAGGAAGGGCCTGCACGAACCCTACAGCGAGAGT
  GACGATGATTGGTGC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC214990
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MARFGDEMPARYGGGGSGAAAGVVVGSGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQ
  NCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEP
  YFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVS
  GIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEE
  PARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLII
  IGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDGEQR
  HPFDGALRRTTIKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRM
  VKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFN
  CFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLL
  FLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVF
  SIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKVEADEQEEEEAANQKLALQKAKEVAEVSPLSAAN
  MSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKAAYTRHLRPDMKTHLDRPLV
  VDPQENRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSGSAGLDARRPWAGSQEAELSR
  EGPYGRESDHHAREGSLEQPGFWEGEAERGKAGDPHRRHVHRQGGSRESRSGSPRTGADGEHRRHRAHR
  RPGEEGPEDKAERRARHREGSRPARGGEGEGEGPDGGERRRRHRHGAPATYEGDARREDKERRHRRRKE
  NQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESAAPHGSLGHAGLPQSPAKMGNS
  TDPGPMLAIPAMATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSGTQTNSAKTARKPDHTTVD
  IPPACPPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEDDRGEDGPKPMPPYSSMFILSTTNPLRRLCHY
  ILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAY
  FRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNV
  FNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNV
  LWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQ
  EQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLM
  MKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNP
  NNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGID
  VEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTRECGNEFAYF
  YFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYLDMYQMLRH
  MSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEM
  MAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQDRTPLMFQRMEPPSP
  TQEGGPGQNALPSTQLDPGGALMAHESGLKESPSWVTQRAQEMFQKTGTWSPEQGPPTDMPNSQPNSQS
  VEMREMGRDGYSDSEHYLPMEGQGRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKAR
  RLDDYSLERVPPEENQRHHQRRRDRSHRASERSLGRYTDVDTGLGTDLSMTTQSGDLPSKERDQERGRP
  KDRKHRQHHHHHHHHHHPPPPDKDRYAQERPDHGRARARDQRWSRSPSEGREHMAHRQGSSSVSGSPAP
  STSGTSTPRRGRRQLPQTPSTPRPHVSYSPVIRKAGGSGPPQQQQQQQQQQQQQAVARPGRAATSGPRR
  YPGPTAEPLAGDRPPTGGHSSGRSPRMERRVPGPARSESPRACRHGGARWPASGPHVSEGPPGPRHHGY
  YRGSDYDEADGPGSGGGEEAMAGAYDAPPPVRHASSGATGRSPRTPRASGPACASPSRHGRRLPNGYYP
  AHGLARPRGPGSRKGLHEPYSESDDDWC
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023035
• ORF Size: 7536 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023035.3
• RefSeq Size: 8646 bp
• RefSeq ORF: 7539 bp
• Locus ID: 773
• UniProt ID: O00555
• Cytogenetics: 19p13.13
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, Long-term depression, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
• MW: 283.1 kDa
• Gene Summary: Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-18 to 21-33 in the coding region is associated with spinocerebellar ataxia 6. [provided by RefSeq, Jul 2016]