PPP1R3A (NM_002711) Human Tagged ORF Clone

CAT#: RC215004

PPP1R3A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A)

Product Images

Western blot validation of overexpression lysate (Cat# LY419154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215004 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP1R3A
• Synonyms: GM; PP1G; PPP1R3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215004 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTTCTGAAGTACCTAGTCAGATTAGCAAAGATAATTTTTTAGAAGTTCCTAATTTATCTGACT
  CTCTTTGTGAAGATGAAGAAGTTACTTTCCAACCTGGTTTCTCCCCTCAACCAAGTAGACGAGGTTCTGA
  TTCTTCTGAAGACATATACCTGGATACCCCATCTTCAGGTACTAGAAGAGTTTCATTTGCTGATTCCTTT
  GGATTCAATCTTGTGTCTGTTAAAGAATTTGATTGCTGGGAATTACCGAGTGCTTCAACCACTTTTGACT
  TAGGGACGGACATTTTCCACACAGAAGAATATGTTTTAGCCCCACTGTTTGACTTGCCTTCTTCAAAAGA
  AGATCTTATGCAACAACTCCAAATACAGAAAGCAATACTGGAGTCAACTGAGTCTCTTCTTGGGTCTACA
  AGTATCAAGGGTATTATTCGAGTTTTGAATGTTTCTTTTGAGAAGTTAGTATATGTAAGAATGTCTTTAG
  ATGACTGGCAGACACATTATGACATTTTAGCAGAATATGTTCCTAATTCATGTGATGGTGAAACTGACCA
  GTTCTCCTTTAAGATTGTATTGGTTCCTCCTTATCAAAAAGATGGCAGTAAAGTTGAGTTTTGTATACGT
  TATGAAACTTCTGTTGGTACATTTTGGTCAAATAATAATGGCACAAATTATACATTCATTTGTCAAAAGA
  AAGAACAAGAGCCGGAGCCTGTAAAACCATGGAAAGAAGTTCCTAACAGACAAATAAAAGGCTGCTTAAA
  GGTAAAATCAAGTAAAGAAGAATCATCAGTAACATCAGAAGAAAATAACTTTGAGAATCCAAAGAATACA
  GATACCTATATCCCAACAATCATTTGTTCTCATGAGGACAAGGAAGATTTGGAAGCCAGTAATCGAAATG
  TAAAAGATGTAAACAGGGAACATGATGAACATAATGAAAAAGAATTAGAGTTGATGATAAATCAACACTT
  AATAAGAACCAGAAGTACTGCTTCCAGAGATGAAAGGAATACATTTTCAACAGATCCAGTCAATTTTCCA
  AATAAAGCAGAGGGGTTAGAGAAGAAGCAAATCCATGGTGAAATATGTACTGACTTGTTCCAAAGGTCTC
  TGTCTCCAAGTTCATCAGCAGAAAGCTCCGTAAAGGGAGATTTTTACTGCAATGAAAAATATTCCTCAGG
  AGATGACTGTACACATCAACCTTCAGAGGAAACTACTTCAAATATGGGAGAAATCAAGCCATCATTGGGA
  GATACTAGTAGTGATGAACTAGTGCAATTACATACTGGCAGCAAAGAAGTCCTGGATGATAATGCTAATC
  CAGCCCATGGCAATGGCACAATGCAAATACCTTGCCCCTCTTCAGATCAACTAATGGCAGGAAACCTTAA
  TAAAAAACATGAAGGAGGAGCTAAAAAAATTGAAGTAAAAGATTTGGGATGTTTACGAAGAGATTTCCAT
  TCAGATACGTCGGCATGTCTCAAAGAATCAACAGAAGAAGGATCTTCTAAGGAAGATTATTATGGCAATG
  GTAAGGATGATGAAGAACAAAGAATATATTTAGGTGTTAATGAAAAACAAAGAAAAAATTTCCAAACAAT
  CTTACATGACCAAGAAAGGAAGATGGGTAACCCTAAAATAAGTGTGGCAGGGATTGGAGCTAGTAACAGA
  GACCTGGCTACTCTGCTGAGCGAACATACCGCAATCCCCACCCGGGCAATCACAGCAGATGTGTCTCATT
  CACCAAGGACAAATTTAAGTTGGGAAGAAGCTGTGTTAACCCCAGAGCATCATCATTTGACTAGTGAAGG
  CAGCGCTTTAGGAGGGATAACTGGTCAAGTTTGTTCATCAAGAACTGGAAATGTTTTGAGGAATGATTAT
  CTTTTCCAAGTTGAAGAAAAATCAGGTGGGATTAATTCTGAAGATCAGGATAATAGCCCACAGCATAAAC
  AAAGTTGGAATGTTCTGGAAAGTCAGGGAAAATCAAGAGAGAATAAGACAAACATAACAGAGCATATCAA
  AGGACAAACAGATTGTGAAGACGTGTGGGGAAAAAGAGATAATACGAGGAGTTTGAAAGCTACTACAGAA
  GAATTGTTTACCTGCCAAGAAACAGTGTGCTGTGAACTGTCTTCTCTAGCTGATCATGGCATTACTGAGA
  AAGCAGAAGCTGGTACAGCCTATATAATTAAGACAACATCAGAAAGTACTCCAGAAAGCATGTCTGCTAG
  AGAAAAAGCAATAATTGCTAAGCTACCTCAAGAGACAGCACGAAGTGACAGGCCCATCGAGGTAAAGGAA
  ACAGCGTTTGATCCACATGAAGGGAGAAATGATGATTCACATTATACCCTTTGTCAACGAGATACAGTAG
  GTGTAATCTATGACAATGATTTTGAAAAGGAATCACGTTTAGGTATTTGTAATGTACGTGTAGATGAAAT
  GGAGAAGGAAGAAACCATGTCTATGTACAATCCTAGGAAGACACATGACAGGGAGAAATGTGGCACTGGA
  AATATAACATCTGTGGAAGAATCCTCATGGGTCATTACAGAATATCAAAAAGCAACTTCAAAACTGGATT
  TACAGTTGGGAATGTTACCAACAGACAAAACTGTATTTTCAGAAAACAGAGATCATAGGCAGGTTCAAGA
  ATTATCAAAGAAAACAGACTCGGATGCCATTGTGCATTCTGCTTTTAACTCAGACACTAATAGAGCTCCT
  CAGAATAGCTCTCCTTTTTCCAAACATCATACTGAAATTTCAGTGTCAACTAATGAGCAGGCAATTGCTG
  TAGAGAATGCAGTTACTACCATGGCTAGCCAACCTATTTCTACGAAATCAGAAAATATTTGTAATTCAAC
  AAGAGAAATCCAGGGTATTGAGAAGCACCCTTATCCTGAGTCTAAACCTGAAGAAGTTTCCAGAAGTTCA
  GGAATAGTGACATCAGGTAGTAGAAAAGAAAGATGCATAGGCCAGATTTTCCAAACAGAAGAGTATAGTG
  TGGAAAAATCTCTAGGGCCAATGATTTTAATCAACAAACCTCTTGAGAATATGGAAGAAGCAAGGCATGA
  AAATGAAGGATTAGTAAGCTCTGGGCAATCACTATACACTTCAGGTGAAAAGGAATCTGACAGCTCTGCT
  TCTACTAGTCTTCCTGTTGAGGAAAGTCAAGCTCAAGGCAACGAATCTTTGTTTTCAAAATATACCAACT
  CTAAAATACCTTATTTCCTTTTGTTTCTGATATTTCTTATAACTGTCTACCATTATGACTTAATGATTGG
  CTTGACATTCTACGTTTTGTCATTGTCCTGGCTATCCTGGGAAGAGGGTAGACAAAAAGAGTCTGTCAAA
  AAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215004 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSF
  GFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGST
  SIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIR
  YETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNT
  DTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFP
  NKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLG
  DTSSDELVQLHTGSKEVLDDNANPAHGNGTMQIPCPSSDQLMAGNLNKKHEGGAKKIEVKDLGCLRRDFH
  SDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNR
  DLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDY
  LFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTE
  ELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKE
  TAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTG
  NITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQELSKKTDSDAIVHSAFNSDTNRAP
  QNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSS
  GIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSA
  STSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVK
  KK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002711
• ORF Size: 3366 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002711.1, NM_002711.2, NM_002711.3, NP_002702.1
• RefSeq Size: 4296 bp
• RefSeq ORF: 3369 bp
• Locus ID: 5506
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Insulin signaling pathway
• MW: 125.8 kDa
• Gene Summary: The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq, Jul 2008]