AMELX (NM_182681) Human Tagged ORF Clone
CAT#: RC215005
AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 2
"NM_182681" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AMELX |
Synonyms | AI1E; AIH1; ALGN; AMG; AMGL; AMGX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215005 representing NM_182681
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCTGGATTTTATTTGCCTGCCTCCTGGGAGCAGCTTTTGCCATGCCTGTGCTTACCCCTTTGA AGTGGTACCAGAGCATAAGGCCACCGTACCCTTCCTATGGTTACGAGCCCATGGGTGGATGGCTGCACCA CCAAATCATCCCCGTGCTGTCCCAACAGCACCCCCCGACTCACACCCTGCAGCCTCATCACCACATCCCA GTGGTGCCAGCTCAGCAGCCCGTGATCCCCCAGCAACCAATGATGCCCGTTCCTGGCCAACACTCCATGA CTCCAATCCAACACCACCAGCCAAACCTCCCTCCGCCCGCCCAGCAGCCCTACCAGCCCCAGCCTGTTCA GCCACAGCCTCACCAGCCCATGCAGCCCCAGCCACCTGTGCACCCCATGCAGCCCCTGCCGCCACAGCCA CCTCTGCCTCCGATGTTCCCCATGCAGCCCCTGCCTCCCATGCTTCCTGATCTGACTCTGGAAGCTTGGC CATCAACAGACAAGACCAAGCGGGAGGAAGTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215005 representing NM_182681
Red=Cloning site Green=Tags(s) MGTWILFACLLGAAFAMPVLTPLKWYQSIRPPYPSYGYEPMGGWLHHQIIPVLSQQHPPTHTLQPHHHIP VVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQPYQPQPVQPQPHQPMQPQPPVHPMQPLPPQP PLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_182681 |
ORF Size | 525 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182681.1, NP_872622.1 |
RefSeq Size | 745 bp |
RefSeq ORF | 528 bp |
Locus ID | 265 |
Cytogenetics | Xp22.2 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
MW | 18.1 kDa |
Gene Summary | 'This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307481 | AMELX (untagged)-Human amelogenin, X-linked (AMELX), transcript variant 2 |
USD 420.00 |
|
RG215005 | AMELX (GFP-tagged) - Human amelogenin, X-linked (AMELX), transcript variant 2 |
USD 460.00 |
|
RC215005L3 | Lenti ORF clone of Human amelogenin, X-linked (AMELX), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC215005L4 | Lenti ORF clone of Human amelogenin, X-linked (AMELX), transcript variant 2, mGFP tagged |
USD 620.00 |
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