AMELX (NM_182681) Human Untagged Clone

CAT#: SC307481

AMELX (untagged)-Human amelogenin, X-linked (AMELX), transcript variant 2


  "NM_182681" in other vectors (4)

Reconstitution Protocol

USD 420.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AMELX
Synonyms AI1E; AIH1; ALGN; AMG; AMGL; AMGX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_182681, the custom clone sequence may differ by one or more nucleotides


ATGGGGACCTGGATTTTATTTGCCTGCCTCCTGGGAGCAGCTTTTGCCATGCCTGTGCTTACCCCTTTGA
AGTGGTACCAGAGCATAAGGCCACCGTACCCTTCCTATGGTTACGAGCCCATGGGTGGATGGCTGCACCA
CCAAATCATCCCCGTGCTGTCCCAACAGCACCCCCCGACTCACACCCTGCAGCCTCATCACCACATCCCA
GTGGTGCCAGCTCAGCAGCCCGTGATCCCCCAGCAACCAATGATGCCCGTTCCTGGCCAACACTCCATGA
CTCCAATCCAACACCACCAGCCAAACCTCCCTCCGCCCGCCCAGCAGCCCTACCAGCCCCAGCCTGTTCA
GCCACAGCCTCACCAGCCCATGCAGCCCCAGCCACCTGTGCACCCCATGCAGCCCCTGCCGCCACAGCCA
CCTCTGCCTCCGATGTTCCCCATGCAGCCCCTGCCTCCCATGCTTCCTGATCTGACTCTGGAAGCTTGGC
CATCAACAGACAAGACCAAGCGGGAGGAAGTGGATTAA


Restriction Sites SgfI-MluI     
ACCN NM_182681
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182681.1, NP_872622.1
RefSeq Size 745 bp
RefSeq ORF 528 bp
Locus ID 265
Cytogenetics Xp22.2
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Gene Summary 'This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) lacks two in-frame exons in the coding region, compared to variant 3. Isoform 2 is shorter than isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.