GSG1 (NM_001080555) Human Tagged ORF Clone

CAT#: RC215112

GSG1 (Myc-DDK-tagged)-Human germ cell associated 1 (GSG1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY421104) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215112 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GSG1
• Synonyms: MGC3146; MGC111023
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215112 representing NM_001080555
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGATCCCTCTCAACTGACTCAAAATGTTTGCCTCACCCAGGAGATGGAGCTCTCGAAGGCCTTCT
  CTGGCCAGCGGACACTCCTATCTGCCATCCTCAGCATGCTATCACTCAGCTTCTCCACAACATCCCTGCT
  CAGCAACTACTGGTTTGTGGGCACACAGAAGGTGCCCAAGCCCCTGTGCGAGAAAGGTCTGGCAGCCAAG
  TGCTTTGACATGCCAGTGTCCCTGGATGGAGATACCAACACATCCACCCAGGAGGTGGTACAATACAACT
  GGGAGACTGGGGATGACCGGTTCTCCTTCCGGAGCTTCCGGAGTGGCATGTGGCTATCCTGTGAGGAAAC
  TGTGGAAGAACCAGCACTGCTCCATCCCCAGTCCTGGAAACAATTTAGAGCCCTTCGGTCCAGTGGTACA
  GCGGCAGCAAAAGGGGAGAGGTGCCGAAGTTTCATTGAACTTACACCACCAGCCAAGAGAGAAATCCTAT
  GGTTATCCCTGGGAACGCAGATCACCTACATCGGACTTCAATTCATCAGCTTCCTCCTGCTACTAACAGA
  CTTGCTACTCACTGGGAACCCTGCCTGTGGGCTCAAACTGAGCGCCTTTGCTGCTGTTTCCTCTGTCCTG
  TCAGGTCTCCTGGGGATGGTGGCCCACATGATGTATTCACAAGTCTTCCAAGCGACTGTCAACTTGGGTC
  CAGAAGACTGGAGACCACATGTTTGGAATTATGGCTGGGCCTTCTACATGGCCTGGCTCTCCTTCACCTG
  CTGCATGGCGTCGGCTGTCACCACCTTCAACACGTACACCAGGATGGTGCTGGAGTTCAAGTGCAAGCAT
  AGTAAGAGCTTCAAGGAAAACCCGAACTGCCTACCACATCACCATCAGTGTTTCCCTCGGCGGCTGTCAA
  GTGCAGCCCCCACCGTGGGTCCTTTGACCAGCTACCACCAGTATCATAATCAGCCCATCCACTCTGTCTC
  TGAGGGAGTCGACTTCTACTCCGAGCTGCGGAACAAGGGATTTCAAAGAGGGGCCAGCCAGGAGCTGAAA
  GAAGCAGTTAGGTCATCTGTAGAGGAAGAGCAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215112 representing NM_001080555
  Red=Cloning site Green=Tags(s)
  
  MSDPSQLTQNVCLTQEMELSKAFSGQRTLLSAILSMLSLSFSTTSLLSNYWFVGTQKVPKPLCEKGLAAK
  CFDMPVSLDGDTNTSTQEVVQYNWETGDDRFSFRSFRSGMWLSCEETVEEPALLHPQSWKQFRALRSSGT
  AAAKGERCRSFIELTPPAKREILWLSLGTQITYIGLQFISFLLLLTDLLLTGNPACGLKLSAFAAVSSVL
  SGLLGMVAHMMYSQVFQATVNLGPEDWRPHVWNYGWAFYMAWLSFTCCMASAVTTFNTYTRMVLEFKCKH
  SKSFKENPNCLPHHHQCFPRRLSSAAPTVGPLTSYHQYHNQPIHSVSEGVDFYSELRNKGFQRGASQELK
  EAVRSSVEEEQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080555
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001080555.1, NM_001080555.2, NP_001074024.1
• RefSeq Size: 2055
• RefSeq ORF: 1089
• Locus ID: 83445
• Protein Families: Transmembrane
• MW: 40.5 kDa
• Gene Summary: May cause the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. [UniProtKB/Swiss-Prot Function]