Cadherin like 23 (CDH23) (NM_052836) Human Tagged ORF Clone

CAT#: RC215133

CDH23 (Myc-DDK-tagged)-Human cadherin-related 23 (CDH23), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409447) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDH23 protein (Cat# TP315133). The protein was produced from HEK293T cells transfected with CDH23 cDNA clone (Cat# RC215133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDH23
• Synonyms: CDHR23; PITA5; USH1D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215133 representing NM_052836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCCATGTTGCCACCAGCTGCCACGTGGCCTGGCTTTTGGTGCTGATCTCTGGATGCTGGGGCC
  AGGTGAACCGGCTGCCCTTCTTCACCAACCACTTCTTTGATACATACCTGCTGATCAGCGAGGACACGCC
  TGTGGGTTCTTCTGTGACCCAGTTGCTGGCCCAAGACATGGACAATGACCCCCTGGTGTTTGGCGTGTCT
  GGGGAGGAGGCCTCTCGCTTCTTTGCAGTGGAGCCTGACACTGGCGTGGTGTGGCTCCGGCAGCCACTGG
  ACAGAGAGACCAAGTCAGAGTTCACCGTGGAGTTCTCTGTCAGCGACCACCAGGGGGTGATCACACGGAA
  GGTGAACATCCAGGTCGGGGATGTGAATGACAACGCGCCCACATTTCACAATCAGCCCTACAGCGTCCGC
  ATCCCTGAGAATACACCAGTGGGGACGCCCATCTTCATCGTGAATGCCACAGACCCCGACTTGGGGGCAG
  GGGGCAGCGTCCTCTACTCCTTCCAGCCCCCCTCCCAATTCTTCGCCATTGACAGCGCCCGCGGTATCGT
  CACAGTGATCCGGGAGCTGGACTACGAGACCACACAGGCCTACCAGCTCACGGTCAACGCCACAGATCAA
  GACAAGACCAGGCCTCTGTCCACCCTGGCCAACTTGGCCATCATCATCACAGATGTCCAGGACATGGACC
  CCATCTTCATCAACCTGCCTTACAGCACCAACATCTACGAGCATTCTCCTCCGGGCACGACGGTGCGCAT
  CATCACCGCCATAGACCAGGATAAAGGACGTCCCCGGGGCATTGGCTACACCATCGTTTCAGGGAATACC
  AACAGCATCTTTGCCCTGGACTACATCAGCGGAGTGCTGACCTTGAATGGCCTGCTGGACCGGGAGAACC
  CCCTGTACAGCCATGGCTTCATCCTGACTGTGAAGGGCACGGAGCTGAACGATGACCGCACCCCATCTGA
  CGCTACAGTCACCACGACCTTCAATATCCTGGTTATTGACATCAATGACAATGCCCCGGAGTTCAACAGC
  TCCGAGTACAGCGTGGCCATCACTGAGCTGGCACAGGTCGGCTTTGCCCTTCCACTCTTCATCCAGGTGG
  TGGACAAGGATGAGAATTTGGGCCTGAACAGCATGTTTGAGGTGTACTTGGTGGGGAACAACTCCCACCA
  CTTCATCATCTCCCCGACCTCCGTCCAGGGGAAGGCGGACATTCGTATTCGGGTGGCCATCCCACTGGAC
  TACGAGACCGTGGACCGCTACGACTTTGATCTCTTTGCCAATGAGAGTGTGCCTGACCATGTGGGCTATG
  CCAAGGTGAAGATCACTCTCATCAATGAAAATGACAACCGGCCCATCTTCAGCCAGCCACTGTACAACAT
  CAGCCTGTACGAGAACGTCACCGTGGGGACCTCTGTGCTGACAGTCCTGGTGAGTCCCCGCTTCACTGCA
  GGGCCACTGAGCTCTCCAGGGCCGACTGTGGTGAGGCACCCAGAGGGATTTTGTCCAAGGGACCTCAGCA
  ATCAGGGAAGGAGGCACCCCCAAATCCCTGAGCTGTGTTTGTTGGTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215133 representing NM_052836
  Red=Cloning site Green=Tags(s)
  
  MGRHVATSCHVAWLLVLISGCWGQVNRLPFFTNHFFDTYLLISEDTPVGSSVTQLLAQDMDNDPLVFGVS
  GEEASRFFAVEPDTGVVWLRQPLDRETKSEFTVEFSVSDHQGVITRKVNIQVGDVNDNAPTFHNQPYSVR
  IPENTPVGTPIFIVNATDPDLGAGGSVLYSFQPPSQFFAIDSARGIVTVIRELDYETTQAYQLTVNATDQ
  DKTRPLSTLANLAIIITDVQDMDPIFINLPYSTNIYEHSPPGTTVRIITAIDQDKGRPRGIGYTIVSGNT
  NSIFALDYISGVLTLNGLLDRENPLYSHGFILTVKGTELNDDRTPSDATVTTTFNILVIDINDNAPEFNS
  SEYSVAITELAQVGFALPLFIQVVDKDENLGLNSMFEVYLVGNNSHHFIISPTSVQGKADIRIRVAIPLD
  YETVDRYDFDLFANESVPDHVGYAKVKITLINENDNRPIFSQPLYNISLYENVTVGTSVLTVLVSPRFTA
  GPLSSPGPTVVRHPEGFCPRDLSNQGRRHPQIPELCLLVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052836
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052836.1, NM_052836.2, NM_052836.3, NP_443068.1
• RefSeq Size: 2022
• RefSeq ORF: 1593
• Locus ID: 64072
• Protein Families: Transmembrane
• MW: 58.4 kDa
• Gene Summary: This gene is a member of the cadherin superfamily, whose genes encode calcium dependent cell-cell adhesion glycoproteins. The encoded protein is thought to be involved in stereocilia organization and hair bundle formation. The gene is located in a region containing the human deafness loci DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of this cadherin-like gene. Upregulation of this gene may also be associated with breast cancer. Alternative splice variants encoding different isoforms have been described. [provided by RefSeq, May 2013]