APLP1 (NM_005166) Human Tagged ORF Clone

CAT#: RC215282

APLP1 (Myc-DDK-tagged)-Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY401581) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215282 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APLP1
• Synonyms: APLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215282 representing NM_005166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCGCCAGCCCCGCTGCTCGCGGTCTAAGTCGCCGCCCGGGCCAGCCGCCGCTGCCGCTGCTGC
  TGCCACTATTGCTGCTGCTTCTGCGCGCGCAGCCCGCCATCGGGAGCCTGGCCGGTGGGAGCCCCGGCGC
  GGCCGAGGCCCCGGGGTCGGCCCAGGTGGCTGGACTATGCGGGCGCCTAACCCTTCACCGGGACCTGCGC
  ACCGGCCGCTGGGAACCAGACCCACAGCGCTCTCGACGCTGTCTCCGGGACCCGCAGCGCGTGCTGGAGT
  ACTGCAGACAGATGTACCCGGAGCTGCAGATTGCACGTGTGGAGCAGGCTACGCAGGCCATCCCCATGGA
  GCGCTGGTGCGGGGGTTCCCGGAGCGGCAGCTGCGCCCACCCCCACCACCAGGTTGTGCCCTTCCGCTGC
  CTGCCTGGTGAATTTGTGAGTGAGGCCCTGCTGGTGCCTGAAGGCTGCCGGTTCTTGCACCAGGAGCGCA
  TGGACCAATGTGAGAGTTCAACCCGGAGGCATCAGGAGGCACAGGAGGCCTGCAGCTCCCAGGGCCTCAT
  CCTGCACGGCTCGGGCATGCTCTTACCCTGTGGCTCGGATCGGTTCCGTGGTGTGGAGTATGTGTGCTGT
  CCCCCTCCAGGGACCCCCGACCCATCTGGGACAGCAGTTGGTGACCCCTCCACCCGGTCCTGGCCCCCGG
  GGAGCAGAGTAGAGGGGGCTGAGGACGAGGAAGAGGAGGAATCCTTCCCACAGCCAGTAGATGATTACTT
  CGTGGAGCCTCCGCAGGCTGAAGAGGAAGAGGAAACGGTCCCACCCCCAAGCTCCCATACACTTGCAGTG
  GTCGGCAAAGTCACTCCCACCCCGAGGCCCACAGACGGTGTGGATATTTACTTTGGCATGCCTGGGGAAA
  TCAGTGAGCACGAGGGGTTCCTGAGGGCCAAGATGGACCTGGAGGAGCGTAGGATGCGCCAGATTAATGA
  GGTGATGCGTGAATGGGCCATGGCAGACAACCAGTCCAAGAACCTGCCTAAAGCCGACAGACAGGCCCTG
  AATGAGCACTTCCAGTCCATTCTGCAGACTCTGGAGGAGCAGGTGTCTGGTGAGCGACAGCGCCTGGTGG
  AAACCCACGCCACCCGCGTCATCGCCCTTATCAACGACCAGCGCCGGGCTGCCTTGGAGGGCTTCCTGGC
  AGCCCTGCAGGCAGATCCGCCTCAGGCGGAGCGTGTCCTGTTGGCCCTGCGGCGCTACCTGCGTGCGGAG
  CAGAAGGAACAGAGGCACACGCTGCGCCACTACCAGCATGTGGCCGCCGTGGATCCCGAGAAGGCACAGC
  AGATGCGCTTCCAGGTGCATACCCACCTTCAAGTGATTGAGGAGAGGGTGAATCAGAGCCTGGGCCTGCT
  TGACCAGAACCCCCACCTGGCTCAGGAGCTGCGGCCCCAAATCCAGGAACTCCTCCACTCTGAACACCTG
  GGTCCCAGTGAATTGGAAGCCCCTGCCCCTGGGGGCAGCAGCGAGGACAAGGGTGGGCTGCAGCCTCCAG
  ATTCCAAGGATGACACCCCCATGACCCTTCCAAAAGGGTCCACAGAACAAGATGCTGCATCCCCTGAGAA
  AGAGAAGATGAACCCGCTGGAACAGTATGAGCGAAAGGTGAATGCGTCTGTTCCAAGGGGTTTCCCTTTC
  CACTCATCGGAGATTCAGAGGGATGAGCTGGCACCAGCTGGGACAGGGGTGTCCCGTGAGGCTGTGTCGG
  GTCTGCTGATCATGGGAGCGGGCGGAGGCTCCCTCATCGTCCTCTCCATGCTGCTCCTGCGCAGGAAGAA
  GCCCTACGGGGCTATCAGCCATGGCGTGGTGGAGGTGGACCCCATGCTGACCCTGGAGGAGCAGCAGCTC
  CGCGAACTGCAGCGGCACGGCTATGAGAACCCCACTTACCGCTTCCTGGAGGAACGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215282 representing NM_005166
  Red=Cloning site Green=Tags(s)
  
  MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLR
  TGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRC
  LPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCC
  PPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAV
  VGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQAL
  NEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAE
  QKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHL
  GPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPF
  HSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTLEEQQL
  RELQRHGYENPTYRFLEERP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005166
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005166.1, NM_005166.2, NM_005166.3, NM_005166.4, NP_005157.1
• RefSeq Size: 2447 bp
• RefSeq ORF: 1953 bp
• Locus ID: 333
• Cytogenetics: 19q13.12
• Domains: A4_EXTRA
• Protein Families: Druggable Genome, Transmembrane
• MW: 72.18 kDa
• Gene Summary: 'This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'