GCNT2 (NM_145655) Human Tagged ORF Clone
CAT#: RC215538
GCNT2 (Myc-DDK-tagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3
"NM_145655" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GCNT2 |
Synonyms | bA360O19.2; bA421M1.1; CCAT; CTRCT13; GCNT2C; GCNT5; IGNT; II; NACGT1; NAGCT1; ULG3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTTGGAGGTACTGCTTTTTTGCTTTCACTCTGCTCAGCGTGGTCATTTTTGTGAGATTTTACA GTAGCCAATTGAGCCCGCCAAAAAGTTATGAGAAGCTGAACAGTTCCAGTGAAAGGTATTTTAGGAAAAC TGCCTGTAATCACGCCTTAGAGAAAATGCCAGTCTTTTTGTGGGAAAATATATTACCATCACCTTTGCGA AGTGTCCCTTGCAAGGATTACCTGACCCAGAATCACTACATCACAAGTCCCCTGTCGGAAGAAGAGGCTG CATTCCCTTTGGCCTATGTCATGGTCATCCATAAGGACTTTGACACCTTTGAAAGGCTCTTTAGGGCTAT CTATATGCCCCAAAATGTCTACTGTGTTCACGTGGATGAGAAAGCCCCAGCTGAGTATAAGGAATCTGTG AGGCAGTTACTGAGTTGCTTCCAAAATGCTTTCATTGCTTCAAAGACAGAGTCTGTGGTTTATGCAGGCA TTTCCAGACTCCAGGCTGACCTGAACTGTCTGAAAGACCTTGTCGCCTCTGAGGTTCCCTGGAAGTACGT CATCAACACCTGTGGACAAGACTTCCCCCTGAAAACCAACCGGGAGATAGTTCAGCATCTGAAAGGATTT AAAGGGAAAAATATCACCCCAGGGGTGCTGCCTCCTGACCATGCAATTAAGCGAACTAAATATGTCCACC AAGAGCATACAGATAAAGGTGGCTTTTTTGTGAAAAATACTAATATTTTGAAAACTTCACCTCCACATCA GCTGACCATCTACTTTGGCACTGCCTATGTGGCGCTTACCAGAGAGTTTGTCGACTTTGTTCTGCGTGAC CAAAGGGCCATTGATCTACTACAATGGTCAAAAGATACCTATAGTCCTGATGAGCATTTCTGGGTGACAC TTAATAGGGTTTCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAACCTCAGAGCTATAAA GTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTATGTACATGGTATTTGTATCTATGGA AACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGCCTGTTTGCTAACAAGTTTGAGCTTAATACCTACC CCCTTACTGTGGAATGCCTAGAACTGAGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACA ACCCAGCTGGTATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215538 protein sequence
Red=Cloning site Green=Tags(s) MNFWRYCFFAFTLLSVVIFVRFYSSQLSPPKSYEKLNSSSERYFRKTACNHALEKMPVFLWENILPSPLR SVPCKDYLTQNHYITSPLSEEEAAFPLAYVMVIHKDFDTFERLFRAIYMPQNVYCVHVDEKAPAEYKESV RQLLSCFQNAFIASKTESVVYAGISRLQADLNCLKDLVASEVPWKYVINTCGQDFPLKTNREIVQHLKGF KGKNITPGVLPPDHAIKRTKYVHQEHTDKGGFFVKNTNILKTSPPHQLTIYFGTAYVALTREFVDFVLRD QRAIDLLQWSKDTYSPDEHFWVTLNRVSGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYG NGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145655 |
ORF Size | 1206 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145655.1, NM_145655.2, NM_145655.3, NP_663630.1, NP_663630.2 |
RefSeq Size | 4219 bp |
RefSeq ORF | 1209 bp |
Locus ID | 2651 |
Cytogenetics | 6p24.3-p24.2 |
Domains | Branch |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways |
MW | 46.5 kDa |
Gene Summary | 'This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC313314 | GCNT2 (untagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3 |
USD 760.00 |
|
RG215538 | GCNT2 (GFP-tagged) - Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3 |
USD 460.00 |
|
RC215538L3 | Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3, Myc-DDK-tagged |
USD 620.00 |
|
RC215538L4 | Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3, mGFP tagged |
USD 620.00 |
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