GCNT2 (NM_145655) Human Tagged ORF Clone

CAT#: RC215538

GCNT2 (Myc-DDK-tagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY407911) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GCNT2
• Synonyms: bA360O19.2; bA421M1.1; CCAT; CTRCT13; GCNT2C; GCNT5; IGNT; II; NACGT1; NAGCT1; ULG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215538 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTTGGAGGTACTGCTTTTTTGCTTTCACTCTGCTCAGCGTGGTCATTTTTGTGAGATTTTACA
  GTAGCCAATTGAGCCCGCCAAAAAGTTATGAGAAGCTGAACAGTTCCAGTGAAAGGTATTTTAGGAAAAC
  TGCCTGTAATCACGCCTTAGAGAAAATGCCAGTCTTTTTGTGGGAAAATATATTACCATCACCTTTGCGA
  AGTGTCCCTTGCAAGGATTACCTGACCCAGAATCACTACATCACAAGTCCCCTGTCGGAAGAAGAGGCTG
  CATTCCCTTTGGCCTATGTCATGGTCATCCATAAGGACTTTGACACCTTTGAAAGGCTCTTTAGGGCTAT
  CTATATGCCCCAAAATGTCTACTGTGTTCACGTGGATGAGAAAGCCCCAGCTGAGTATAAGGAATCTGTG
  AGGCAGTTACTGAGTTGCTTCCAAAATGCTTTCATTGCTTCAAAGACAGAGTCTGTGGTTTATGCAGGCA
  TTTCCAGACTCCAGGCTGACCTGAACTGTCTGAAAGACCTTGTCGCCTCTGAGGTTCCCTGGAAGTACGT
  CATCAACACCTGTGGACAAGACTTCCCCCTGAAAACCAACCGGGAGATAGTTCAGCATCTGAAAGGATTT
  AAAGGGAAAAATATCACCCCAGGGGTGCTGCCTCCTGACCATGCAATTAAGCGAACTAAATATGTCCACC
  AAGAGCATACAGATAAAGGTGGCTTTTTTGTGAAAAATACTAATATTTTGAAAACTTCACCTCCACATCA
  GCTGACCATCTACTTTGGCACTGCCTATGTGGCGCTTACCAGAGAGTTTGTCGACTTTGTTCTGCGTGAC
  CAAAGGGCCATTGATCTACTACAATGGTCAAAAGATACCTATAGTCCTGATGAGCATTTCTGGGTGACAC
  TTAATAGGGTTTCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAACCTCAGAGCTATAAA
  GTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTATGTACATGGTATTTGTATCTATGGA
  AACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGCCTGTTTGCTAACAAGTTTGAGCTTAATACCTACC
  CCCTTACTGTGGAATGCCTAGAACTGAGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACA
  ACCCAGCTGGTATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215538 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFWRYCFFAFTLLSVVIFVRFYSSQLSPPKSYEKLNSSSERYFRKTACNHALEKMPVFLWENILPSPLR
  SVPCKDYLTQNHYITSPLSEEEAAFPLAYVMVIHKDFDTFERLFRAIYMPQNVYCVHVDEKAPAEYKESV
  RQLLSCFQNAFIASKTESVVYAGISRLQADLNCLKDLVASEVPWKYVINTCGQDFPLKTNREIVQHLKGF
  KGKNITPGVLPPDHAIKRTKYVHQEHTDKGGFFVKNTNILKTSPPHQLTIYFGTAYVALTREFVDFVLRD
  QRAIDLLQWSKDTYSPDEHFWVTLNRVSGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYG
  NGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145655
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145655.1, NM_145655.2, NM_145655.3, NP_663630.1, NP_663630.2
• RefSeq Size: 4219 bp
• RefSeq ORF: 1209 bp
• Locus ID: 2651
• Cytogenetics: 6p24.3-p24.2
• Domains: Branch
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 46.5 kDa
• Gene Summary: 'This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'