GCNT2 (NM_145655) Human Untagged Clone

CAT#: SC313314

GCNT2 (untagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCNT2
• Synonyms: bA360O19.2; bA421M1.1; CCAT; CTRCT13; GCNT2C; GCNT5; IGNT; II; NACGT1; NAGCT1; ULG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145655, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACTTTTGGAGGTACTGCTTTTTTGCTTTCACTCTGCTCAGCGTGGTCATTTTTGTGAGATTTTACA
  GTAGCCAATTGAGCCCGCCAAAAAGTTATGAGAAGCTGAACAGTTCCAGTGAAAGGTATTTTAGGAAAAC
  TGCCTGTAATCACGCCTTAGAGAAAATGCCAGTCTTTTTGTGGGAAAATATATTACCATCACCTTTGCGA
  AGTGTCCCTTGCAAGGATTACCTGACCCAGAATCACTACATCACAAGTCCCCTGTCGGAAGAAGAGGCTG
  CATTCCCTTTGGCCTATGTCATGGTCATCCATAAGGACTTTGACACCTTTGAAAGGCTCTTTAGGGCTAT
  CTATATGCCCCAAAATGTCTACTGTGTTCACGTGGATGAGAAAGCCCCAGCTGAGTATAAGGAATCTGTG
  AGGCAGTTACTGAGTTGCTTCCAAAATGCTTTCATTGCTTCAAAGACAGAGTCTGTGGTTTATGCAGGCA
  TTTCCAGACTCCAGGCTGACCTGAACTGTCTGAAAGACCTTGTCGCCTCTGAGGTTCCCTGGAAGTACGT
  CATCAACACCTGTGGACAAGACTTCCCCCTGAAAACCAACCGGGAGATAGTTCAGCATCTGAAAGGATTT
  AAAGGGAAAAATATCACCCCAGGGGTGCTGCCTCCTGACCATGCAATTAAGCGAACTAAATATGTCCACC
  AAGAGCATACAGATAAAGGTGGCTTTTTTGTGAAAAATACTAATATTTTGAAAACTTCACCTCCACATCA
  GCTGACCATCTACTTTGGCACTGCCTATGTGGCGCTTACCAGAGACTTTGTCGACTTTGTTCTACGTGAC
  CAAAGGGCCATTGATCTACTACAATGGTCAAAAGATACCTATAGTCCTGATGAGCATTTCTGGGTGACAC
  TTAATAGGGTTTCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAACCTCAGAGCTATAAA
  GTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTATGTACATGGTATTTGTATCTATGGA
  AACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGCCTGTTTGCTAACAAGTTTGAGCTTAATACCTACC
  CCCTTACTGTGGAATGCCTAGAACTGAGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACA
  ACCCAGCTGGTATTTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_145655
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145655.3, NP_663630.2
• RefSeq Size: 4219 bp
• RefSeq ORF: 1209 bp
• Locus ID: 2651
• Cytogenetics: 6p24.3-p24.2
• Domains: Branch
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• Gene Summary: 'This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) contains a different 5' end exon compared to variant 2. The encoded isoform (C) is longer and has a distinct N-terminus, compared to isoform B. Sequence Note: This RefSeq record represents the GCNT2*001.1.1 allele.