ZnT1 (SLC30A1) (NM_021194) Human Tagged ORF Clone

CAT#: RC215616

SLC30A1 (Myc-DDK-tagged)-Human solute carrier family 30 (zinc transporter), member 1 (SLC30A1)

Product Images

Western blot validation of overexpression lysate (Cat# LY412033) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215616 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLC30A1 protein (Cat# TP315616). The protein was produced from HEK293T cells transfected with SLC30A1 cDNA clone (Cat# RC215616) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC30A1
• Synonyms: ZNT1; ZRC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215616 representing NM_021194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGTTGGGGTCGGAACCGGGGCCGGCTGCTGTGCATGCTGGCGCTGACCTTCATGTTCATGGTGC
  TGGAGGTGGTGGTGAGCCGGGTGACCTCGTCGCTGGCGATGCTCTCCGACTCCTTCCACATGCTGTCGGA
  CGTGCTGGCGCTGGTGGTGGCGCTGGTGGCCGAGCGCTTCGCCCGGCGGACCCACGCCACCCAGAAGAAC
  ACGTTCGGCTGGATCCGAGCCGAGGTAATGGGGGCTCTGGTGAACGCCATCTTCCTGACTGGCCTCTGTT
  TCGCCATCCTGCTGGAGGCCATCGAGCGCTTCATCGAGCCGCACGAGATGCAGCAGCCGCTGGTGGTCCT
  TGGGGTCGGCGTGGCCGGGCTGCTGGTCAACGTGCTGGGGCTCTGCCTCTTCCACCATCACAGCGGCTTC
  AGCCAGGACTCCGGCCACGGCCACTCGCACGGGGGTCACGGCCACGGCCACGGCCTCCCCAAGGGGCCTC
  GCGTTAAGAGCACCCGCCCCGGGAGCAGCGACATCAACGTGGCCCCGGGCGAGCAGGGTCCCGACCAGGA
  GGAGACCAACACCCTGGTGGCCAATACCAGCAACTCCAACGGGCTGAAATTGGACCCCGCAGACCCAGAA
  AACCCCAGAAGTGGTGATACAGTGGAAGTACAAGTGAATGGAAATCTTGTCAGAGAACCTGACCATATGG
  AACTGGAAGAAGATAGGGCTGGACAACTTAACATGCGTGGAGTTTTTCTGCATGTCCTTGGAGATGCCTT
  GGGTTCAGTGATTGTAGTAGTAAATGCCTTAGTCTTTTACTTTTCTTGGAAAGGTTGTTCTGAAGGGGAT
  TTTTGTGTGAATCCATGTTTCCCTGACCCCTGCAAAGCATTTGTAGAAATAATTAATAGTACTCATGCAT
  CAGTTTATGAGGCTGGTCCTTGCTGGGTGCTATATTTAGATCCAACTCTTTGTGTTGTAATGGTTTGTAT
  ACTTCTTTACACAACCTATCCATTACTTAAGGAATCTGCTCTTATTCTTCTACAAACTGTTCCTAAACAA
  ATTGATATCAGAAATTTGATAAAAGAACTTCGAAATGTTGAAGGAGTTGAGGAAGTTCATGAATTACATG
  TTTGGCAACTTGCTGGAAGCAGAATCATTGCCACTGCTCACATAAAATGTGAAGATCCAACATCATACAT
  GGAGGTGGCTAAAACCATTAAAGACGTTTTTCATAATCACGGAATTCACGCTACTACCATTCAGCCTGAA
  TTTGCTAGTGTAGGCTCTAAATCAAGTGTAGTTCCGTGTGAACTTGCCTGCAGAACCCAGTGTGCTTTGA
  AGCAATGTTGTGGGACACTACCACAAGCCCCTTCTGGAAAGGATGCAGAAAAGACCCCAGCAGTTAGCAT
  TTCTTGTTTAGAACTTAGTAACAATCTAGAGAAGAAGCCCAGGAGGACTAAAGCTGAAAACATCCCTGCT
  GTTGTGATAGAGATTAAAAACATGCCAAACAAACAACCTGAATCATCTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215616 representing NM_021194
  Red=Cloning site Green=Tags(s)
  
  MGCWGRNRGRLLCMLALTFMFMVLEVVVSRVTSSLAMLSDSFHMLSDVLALVVALVAERFARRTHATQKN
  TFGWIRAEVMGALVNAIFLTGLCFAILLEAIERFIEPHEMQQPLVVLGVGVAGLLVNVLGLCLFHHHSGF
  SQDSGHGHSHGGHGHGHGLPKGPRVKSTRPGSSDINVAPGEQGPDQEETNTLVANTSNSNGLKLDPADPE
  NPRSGDTVEVQVNGNLVREPDHMELEEDRAGQLNMRGVFLHVLGDALGSVIVVVNALVFYFSWKGCSEGD
  FCVNPCFPDPCKAFVEIINSTHASVYEAGPCWVLYLDPTLCVVMVCILLYTTYPLLKESALILLQTVPKQ
  IDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPE
  FASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPA
  VVIEIKNMPNKQPESSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021194
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021194.1, NM_021194.2, NP_067017.2
• RefSeq Size: 2034 bp
• RefSeq ORF: 1524 bp
• Locus ID: 7779
• Domains: Cation_efflux
• Protein Families: Transmembrane
• MW: 55.1 kDa
• Gene Summary: May be involved in zinc transport out of the cell. [UniProtKB/Swiss-Prot Function]