SPIRE2 (NM_032451) Human Tagged ORF Clone

CAT#: RC215650

SPIRE2 (Myc-DDK-tagged)-Human spire homolog 2 (Drosophila) (SPIRE2)


  "NM_032451" in other vectors (6)

Reconstitution Protocol

USD 610.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SPIRE2
Synonyms Spir-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215650 representing NM_032451
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGGCGGGCAGCTGCGGCGGCGCCGCGGCGGGCGCAGGGCGGCCGGAGCCCTGGGAGCTGTCCC
TGGAGGAGGTGCTGAAGGCCTACGAGCAGCCGCTCAACGAGGAGCAGGCGTGGGCCGTGTGCTTCCAGGG
CTGCCGCGGGCTGCGGGGCTCGCCGGGCCGGCGCCTGCGGGATACCGGGGACCTCCTGCTGCGCGGGGAC
GGCTCGGTCGGGGCGCGGGAGCCCGAGGCCGCGGAACCTGCAACCATGGTCGTGCCACTAGCCAGCTCGG
AAGCCCAGACCGTGCAGTCCCTCGGCTTCGCCATCTACCGCGCGCTGGACTGGGGGCTGGACGAGAGCGA
GGAGCGCGAACTCAGCCCTCAGCTGGAGCGGCTCATCGACCTCATGGCCAACAACGACAGCGAGGACAGC
GGCTGCGGTGCCGCCGATGAGGGCTACGGGGGTCCCGAGGAGGAGGAGGAGGCCGAGGGCGTCCCCCGCA
GCGTGCGCACCTTTGCCCAGGCCATGCGGCTGTGCGCGGCGCGGCTGACCGACCCCCGGGGCGCACAGGC
GCATTACCAGGCCGTGTGCCGCGCGCTCTTCGTGGAGACGCTGGAGCTGCGGGCCTTCCTGGCCAGGGTC
CGGGAGGCCAAGGAGATGCTGCAGAAGCTTCGGGAGGACGAGCCGCATCTGGAGACGCCTCGGGCAGAGC
TGGACAGCCTGGGTCACACAGACTGGGCCCGACTGTGGGTTCAGCTCATGCGGGAGCTCCGCCGCGGAGT
GAAGCTGAAGAAGGTGCAAGAGCAGGAGTTCAACCCCCTCCCCACCGAGTTCCAGCTCACGCCCTTCGAG
ATGCTGATGCAGGACATCCGGGCCCGGAACTACAAGCTGCGCAAGGTCATGGTGGATGGGGACATCCCGC
CCCGGGTGAAGAAGGACGCTCACGAGCTCATCCTGGACTTTATCCGCTCACGGCCTCCACTGAAGCAGGT
CTCTGAGAGGCGGCTGCGCCCGTTGCCACCAAAGCAAAGGTCCCTGCATGAGAAGATCCTGGAGGAGATC
AAGCAGGAGCGGAGGCTGCGCCCGGTGCGGGGCGAGGGCTGGGCTGCCCGCGGGTTTGGCTCTCTGCCCT
GCATCCTCAACGCCTGCTCCGGAGATGCCAAGTCCACCTCCTGCATCAACCTGTCAGTCACAGATGCTGG
GGGCAGCGCCCAGCGCCCGCGGCCCCGCGTGCTGCTCAAGGCGCCTACCTTGGCTGAAATGGAAGAGATG
AATACATCTGAGGAAGAAGAGTCTCCGTGTGGGGAGGTGACGCTGAAACGGGACCGCTCCTTCTCAGAGC
ATGACCTGGCCCAGCTCCGAAGTGAGGTGGCCTCTGGCCTGCAGTCGGCCACCCACCCCCCAGGAGGGAC
GGAGCCACCACGGCCCCGAGCTGGCAGTGCGCATGTGTGGAGGCCCGGCTCCCGAGACCAGGGTACCTGT
CCCGCGAGTGTCTCTGACCCCAGCCACCCCCTACTCAGCAACCGGGGCTCCTCGGGGGACAGACCCGAGG
CCTCCATGACCCCCGATGCCAAACACCTGTGGCTGGAGTTCAGCCACCCCGTGGAGAGCCTGGCGCTGAC
TGTGGAAGAGGTGATGGACGTGCGCCGTGTGCTGGTGAAGGCCGAGATGGAAAAGTTTTTGCAGAACAAG
GAGCTCTTCAGCAGTCTGAAGAAGGGGAAGATTTGCTGCTGCTGCCGGGCCAAGTTCCCGCTGTTCTCGT
GGCCGCCCAGCTGTCTCTTCTGCAAGAGAGCCGTCTGCACTTCCTGTAGCATAAAGATGAAGATGCCTTC
TAAGAAATTTGGACACATCCCTGTCTACACACTGGGCTTTGAGAGTCCTCAGAGGGTATCAGCTGCCAAA
ACCGCGCCAATCCAGAGAAGAGACATCTTTCAGTCTCTGCAAGGGCCACAGTGGCAGAGCGTGGAGGAGG
CGTTCCCCCACATCTACTCCCACGGCTGTGTCCTGAAGGATGTCTGCAGTGAGTGCACCAGCTTTGTGGC
AGACGTGGTGCGTTCCAGCCGCAAGAGCGTGGACGTCCTCAACACTACGCCACGACGCAGTCGCCAGACC
CAATCCCTCTACATCCCTAACACCAGGACTCTTGACTTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215650 representing NM_032451
Red=Cloning site Green=Tags(s)

MARAGSCGGAAAGAGRPEPWELSLEEVLKAYEQPLNEEQAWAVCFQGCRGLRGSPGRRLRDTGDLLLRGD
GSVGAREPEAAEPATMVVPLASSEAQTVQSLGFAIYRALDWGLDESEERELSPQLERLIDLMANNDSEDS
GCGAADEGYGGPEEEEEAEGVPRSVRTFAQAMRLCAARLTDPRGAQAHYQAVCRALFVETLELRAFLARV
REAKEMLQKLREDEPHLETPRAELDSLGHTDWARLWVQLMRELRRGVKLKKVQEQEFNPLPTEFQLTPFE
MLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERRLRPLPPKQRSLHEKILEEI
KQERRLRPVRGEGWAARGFGSLPCILNACSGDAKSTSCINLSVTDAGGSAQRPRPRVLLKAPTLAEMEEM
NTSEEEESPCGEVTLKRDRSFSEHDLAQLRSEVASGLQSATHPPGGTEPPRPRAGSAHVWRPGSRDQGTC
PASVSDPSHPLLSNRGSSGDRPEASMTPDAKHLWLEFSHPVESLALTVEEVMDVRRVLVKAEMEKFLQNK
ELFSSLKKGKICCCCRAKFPLFSWPPSCLFCKRAVCTSCSIKMKMPSKKFGHIPVYTLGFESPQRVSAAK
TAPIQRRDIFQSLQGPQWQSVEEAFPHIYSHGCVLKDVCSECTSFVADVVRSSRKSVDVLNTTPRRSRQT
QSLYIPNTRTLDFK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032451
ORF Size 2142 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032451.1, NP_115827.1
RefSeq Size 3249 bp
RefSeq ORF 2145 bp
Locus ID 84501
Protein Pathways Dorso-ventral axis formation
MW 79.5 kDa

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