KLF7 (NM_003709) Human Tagged ORF Clone
CAT#: RC215688
KLF7 (Myc-DDK-tagged)-Human Kruppel-like factor 7 (ubiquitous) (KLF7)
"NM_003709" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KLF7 |
Synonyms | UKLF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215688 representing NM_003709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGTTGGCTAGTTATAGTATATTCCAGGAGCTACAACTTGTCCACGACACCGGCTACTTCTCAG CTTTACCATCCCTGGAGGAGACCTGGCAGCAGACATGCCTTGAATTGGAACGCTACCTACAGACGGAGCC CCGGAGGATCTCAGAGACCTTTGGTGAGGACTTGGACTGTTTCCTCCACGCTTCCCCTCCCCCGTGCATT GAGGAAAGCTTCCGTCGCTTAGACCCCCTGCTGCTCCCCGTGGAAGCGGCCATCTGTGAGAAGAGCTCGG CAGTGGACATCTTGCTCTCTCGGGACAAGTTGCTATCTGAGACCTGCCTCAGCCTCCAGCCGGCCAGCTC TTCTCTAGACAGCTACACAGCCGTCAACCAGGCCCAGCTCAACGCAGTGACCTCATTAACGCCCCCATCG TCCCCTGAGCTCAGCCGCCATCTGGTCAAAACCTCACAAACTCTCTCTGCCGTGGATGGCACGGTGACGT TGAAACTGGTGGCCAAGAAGGCTGCTCTCAGCTCCGTAAAGGTGGGAGGGGTCGCAACAGCTGCAGCAGC CGTGACGGCTGCGGGGGCCGTTAAGAGTGGACAGAGCGACAGTGACCAAGGAGGGCTAGGGGCTGAAGCA TGTCCCGAAAACAAGAAGAGGGTTCACCGCTGTCAGTTTAACGGGTGCCGGAAAGTTTATACAAAAAGCT CCCACTTAAAGGCCCACCAGAGGACTCACACAGGTGAGAAGCCTTATAAGTGCTCATGGGAGGGATGTGA GTGGCGTTTTGCACGAAGCGATGAGCTCACGAGGCACTACAGGAAACACACAGGTGCAAAGCCCTTCAAA TGCAACCACTGCGACAGGTGTTTTTCCAGGTCTGACCATCTTGCCCTCCACATGAAGAGACATATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215688 representing NM_003709
Red=Cloning site Green=Tags(s) MDVLASYSIFQELQLVHDTGYFSALPSLEETWQQTCLELERYLQTEPRRISETFGEDLDCFLHASPPPCI EESFRRLDPLLLPVEAAICEKSSAVDILLSRDKLLSETCLSLQPASSSLDSYTAVNQAQLNAVTSLTPPS SPELSRHLVKTSQTLSAVDGTVTLKLVAKKAALSSVKVGGVATAAAAVTAAGAVKSGQSDSDQGGLGAEA CPENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFK CNHCDRCFSRSDHLALHMKRHI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003709 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003709.1, NM_003709.2, NM_003709.3, NP_003700.1 |
RefSeq Size | 1640 bp |
RefSeq ORF | 909 bp |
Locus ID | 8609 |
Domains | zf-C2H2 |
Protein Families | Transcription Factors |
MW | 33.2 kDa |
Gene Summary | The protein encoded by this gene is a member of the Kruppel-like transcriptional regulator family. Members in this family regulate cell proliferation, differentiation and survival and contain three C2H2 zinc fingers at the C-terminus that mediate binding to GC-rich sites. This protein may contribute to the progression of type 2 diabetes by inhibiting insulin expression and secretion in pancreatic beta-cells and by deregulating adipocytokine secretion in adipocytes. A pseudogene of this gene is located on the long arm of chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111078 | KLF7 (untagged)-Human Kruppel-like factor 7 (ubiquitous) (KLF7) |
USD 660.00 |
|
RG215688 | KLF7 (GFP-tagged) - Human Kruppel-like factor 7 (ubiquitous) (KLF7) |
USD 460.00 |
|
RC215688L1 | Lenti ORF clone of Human Kruppel-like factor 7 (ubiquitous) (KLF7), Myc-DDK-tagged |
USD 768.00 |
|
RC215688L2 | Lenti ORF clone of Human Kruppel-like factor 7 (ubiquitous) (KLF7), mGFP tagged |
USD 620.00 |
|
RC215688L3 | Lenti ORF clone of Human Kruppel-like factor 7 (ubiquitous) (KLF7), Myc-DDK-tagged |
USD 620.00 |
|
RC215688L4 | Lenti ORF clone of Human Kruppel-like factor 7 (ubiquitous) (KLF7), mGFP tagged |
USD 620.00 |
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