SH3KBP1 (NM_001024666) Human Tagged ORF Clone

CAT#: RC215701

SH3KBP1 (Myc-DDK-tagged)-Human SH3-domain kinase binding protein 1 (SH3KBP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY422529) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215701 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SH3KBP1 protein (Cat# TP315701). The protein was produced from HEK293T cells transfected with SH3KBP1 cDNA clone (Cat# RC215701) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH3KBP1
• Synonyms: AGMX2; CD2BP3; CIN85; GIG10; HSB-1; HSB1; IMD61; MIG18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215701 representing NM_001024666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTTTCAGCAGCCAAAGCCCCAAGTGCCGCAGACTTGTCTGAGATTGAAATAAAGAAAGAGATGA
  AGAAAGACCCTCTCACCAACAAAGCTCCAGAAAAGCCCCTGCACGAAGTGCCCAGTGGAAACTCTTTGCT
  GTCTTCTGAAACGATTTTAAGAACCAATAAGAGAGGCGAGCGACGGAGGCGCCGGTGCCAGGTGGCATTC
  AGCTACCTGCCCCAGAATGACGATGAACTTGAGCTGAAAGTTGGCGACATCATAGAGGTGGTAGGAGAGG
  TAGAGGAAGGATGGTGGGAAGGTGTTCTCAACGGGAAGACTGGAATGTTTCCTTCCAACTTCATCAAGGA
  GCTGTCAGGGGAGTCGGATGAGCTTGGCATTTCCCAGGATGAGCAGCTATCCAAGTCAAGTTTAAGGGAA
  ACCACAGGCTCCGAGAGTGATGGGGGTGACTCAAGCAGCACCAAGTCTGAAGGTGCCAACGGGACAGTGG
  CAACTGCAGCAATCCAGCCCAAGAAAGTTAAGGGAGTGGGCTTTGGAGACATTTTCAAAGACAAGCCAAT
  CAAACTAAGACCAAGGTCAATTGAAGTAGAAAATGACTTTCTGCCGGTAGAAAAGACTATTGGGAAGAAG
  TTACCTGCAACTACAGCAACTCCAGACTCATCAAAAACAGAAATGGACAGCAGGACAAAGAGCAAGGATT
  ACTGCAAAGTAATATTTCCATATGAGGCACAGAATGATGATGAATTGACAATCAAAGAAGGAGATATAGT
  CACTCTCATCAATAAGGACTGCATCGACGTAGGCTGGTGGGAAGGAGAGCTGAACGGCAGACGAGGCGTG
  TTCCCCGATAACTTCGTGAAGTTACTTCCACCGGACTTTGAAAAGGAAGGGAATAGACCCAAGAAGCCAC
  CGCCTCCATCCGCTCCTGTCATCAAACAAGGGGCAGGCACCACTGAGAGAAAACATGAAATTAAAAAGAT
  ACCTCCTGAAAGACCAGAAATGCTTCCAAACAGAACAGAAGAAAAAGAAAGACCAGAGAGAGAGCCAAAA
  CTGGATTTACAGAAGCCCTCCGTTCCTGCCATACCGCCAAAAAAGCCTCGGCCACCTAAGACCAATTCTC
  TCAGCAGACCTGGCGCACTGCCCCCGAGAAGGCCGGAGAGACCGGTGGGTCCGCTGACACACACCAGGGG
  TGACAGTCCAAAGATTGACTTGGCCGGCAGTTCGCTATCTGGCATCCTGGACAAAGATCTCTCGGACCGC
  AGCAATGACATTGACTTAGAAGGTTTTGACTCCGTGGTATCATCTACTGAGAAACTCAGTCATCCGACCA
  CAAGCAGACCAAAAGCTACAGGGAGGCGGCCTCCGTCCCAGTCCCTCACATCTTCATCCCTTTCAAGCCC
  TGATATCTTCGACTCCCCAAGTCCCGAAGAGGATAAGGAGGAACACATTTCACTTGCGCACAGAGGAGTG
  GACGCGTCAAAGAAAACTTCCAAGACTGTTACCATATCCCAAGTGTCTGACAACAAAGCATCCCTGCCGC
  CCAAGCCGGGGACCATGGCAGCAGGTGGCGGTGGGCCAGCCCCTCTGTCCTCAGCGGCGCCCTCCCCCCT
  GTCATCCTCTTTGGGAACAGCTGGACACAGAGCCAACTCCCCGTCTCTGTTCGGCACGGAAGGAAAACCA
  AAGATGGAGCCTGCGGCCAGCAGCCAGGCGGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGGA
  GCATCATCGAGACCATGAAGGACCAGCAGAAACGAGAGATTAAACAGTTATTGTCTGAGTTGGATGAAGA
  GAAGAAAATCCGGCTTCGGTTGCAGATGGAAGTGAACGACATAAAGAAAGCTCTACAATCAAAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215701 representing NM_001024666
  Red=Cloning site Green=Tags(s)
  
  MEVSAAKAPSAADLSEIEIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAF
  SYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRE
  TTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKK
  LPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGV
  FPDNFVKLLPPDFEKEGNRPKKPPPPSAPVIKQGAGTTERKHEIKKIPPERPEMLPNRTEEKERPEREPK
  LDLQKPSVPAIPPKKPRPPKTNSLSRPGALPPRRPERPVGPLTHTRGDSPKIDLAGSSLSGILDKDLSDR
  SNDIDLEGFDSVVSSTEKLSHPTTSRPKATGRRPPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGV
  DASKKTSKTVTISQVSDNKASLPPKPGTMAAGGGGPAPLSSAAPSPLSSSLGTAGHRANSPSLFGTEGKP
  KMEPAASSQAAVEELRTQVRELRSIIETMKDQQKREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024666
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024666.1, NM_001024666.2, NP_001019837.1
• RefSeq Size: 2689
• RefSeq ORF: 1887
• Locus ID: 30011
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 68.4 kDa
• Gene Summary: This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]