MATN1 (NM_002379) Human Tagged ORF Clone

CAT#: RC215778

MATN1 (Myc-DDK-tagged)-Human matrilin 1, cartilage matrix protein (MATN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419364) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MATN1
• Synonyms: CMP; CRTM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215778 representing NM_002379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTCCTCTCTGGCACTAGCCTCATGCTCTGCAGCCTGCTGCTGCTGCTCCAGGCCCTGTGCAGCC
  CTGGCCTCGCCCCCCAGTCCAGAGGCCATCTCTGCCGGACGCGGCCCACAGACCTGGTGTTTGTTGTCGA
  CAGCTCTCGCAGCGTTCGGCCTGTTGAATTTGAGAAAGTGAAGGTATTCCTGTCCCAGGTCATCGAGTCG
  CTGGACGTGGGGCCCAATGCCACCCGGGTGGGCATGGTCAACTATGCCAGCACCGTGAAGCAGGAGTTCT
  CGCTGCGGGCTCATGTCTCCAAGGCCGCACTGCTGCAGGCTGTGCGCCGTATCCAGCCGCTGTCCACAGG
  CACCATGACCGGCCTGGCCATCCAGTTCGCTATCACCAAAGCCTTCGGCGATGCAGAGGGTGGTCGTTCC
  AGGTCCCCTGACATCAGCAAGGTGGTCATCGTGGTGACAGACGGGAGGCCCCAGGACAGCGTGCAGGACG
  TGTCTGCGCGGGCCCGGGCCAGCGGCGTCGAGCTGTTCGCCATCGGAGTGGGCAGCGTGGACAAGGCCAC
  GCTGCGGCAGATCGCCAGCGAGCCGCAGGACGAACACGTCGATTACGTGGAGAGCTACAGCGTCATCGAG
  AAGCTGTCCAGGAAGTTCCAGGAGGCCTTCTGCGTGGTGTCAGACCTGTGCGCCACAGGGGACCATGACT
  GTGAGCAGGTGTGCATCAGCTCCCCCGGTTCCTACACCTGCGCCTGCCACGAGGGCTTCACTCTGAACAG
  CGACGGCAAGACCTGCAATGTCTGCAGTGGTGGTGGTGGCAGCTCGGCCACTGACCTGGTCTTCCTCATT
  GACGGATCCAAGAGTGTGAGGCCAGAGAACTTTGAGCTGGTGAAGAAGTTCATCAGTCAGATCGTGGATA
  CGCTGGACGTGTCAGACAAGCTGGCCCAGGTGGGGCTGGTGCAGTACTCAAGCTCTGTGCGCCAGGAGTT
  CCCCCTGGGTCGCTTCCACACCAAGAAGGACATCAAGGCGGCTGTGCGGAATATGTCCTACATGGAGAAG
  GGCACAATGACTGGGGCTGCTCTCAAGTACCTCATTGACAATTCCTTCACTGTGTCCAGTGGGGCTAGGC
  CCGGGGCCCAGAAGGTGGGCATTGTCTTCACTGATGGCCGGAGCCAGGACTACATTAATGATGCTGCCAA
  GAAGGCCAAAGACCTCGGCTTTAAGATGTTTGCTGTGGGTGTGGGCAATGCCGTGGAGGATGAGCTGAGG
  GAAATAGCCTCAGAGCCTGTGGCAGAGCACTACTTCTACACGGCTGACTTCAAGACCATCAACCAGATAG
  GCAAGAAGTTGCAGAAGAAGATCTGTGTGGAGGAAGACCCGTGTGCCTGCGAGTCCCTGGTGAAATTCCA
  AGCCAAAGTGGAGGGGCTGCTGCAGGCCCTGACCAGGAAACTGGAAGCTGTGAGTAAGCGGCTGGCCATC
  CTGGAGAACACAGTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215778 representing NM_002379
  Red=Cloning site Green=Tags(s)
  
  MRVLSGTSLMLCSLLLLLQALCSPGLAPQSRGHLCRTRPTDLVFVVDSSRSVRPVEFEKVKVFLSQVIES
  LDVGPNATRVGMVNYASTVKQEFSLRAHVSKAALLQAVRRIQPLSTGTMTGLAIQFAITKAFGDAEGGRS
  RSPDISKVVIVVTDGRPQDSVQDVSARARASGVELFAIGVGSVDKATLRQIASEPQDEHVDYVESYSVIE
  KLSRKFQEAFCVVSDLCATGDHDCEQVCISSPGSYTCACHEGFTLNSDGKTCNVCSGGGGSSATDLVFLI
  DGSKSVRPENFELVKKFISQIVDTLDVSDKLAQVGLVQYSSSVRQEFPLGRFHTKKDIKAAVRNMSYMEK
  GTMTGAALKYLIDNSFTVSSGARPGAQKVGIVFTDGRSQDYINDAAKKAKDLGFKMFAVGVGNAVEDELR
  EIASEPVAEHYFYTADFKTINQIGKKLQKKICVEEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAI
  LENTVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002379
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002379.1, NM_002379.2, NM_002379.3, NP_002370.1
• RefSeq Size: 2414 bp
• RefSeq ORF: 1491 bp
• Locus ID: 4146
• Cytogenetics: 1p35.2
• MW: 53.5 kDa
• Gene Summary: 'This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. [provided by RefSeq, Jul 2008]'