SMC1 (SMC1A) (NM_006306) Human Tagged ORF Clone

CAT#: RC215888L4

Lenti ORF clone of Human structural maintenance of chromosomes 1A (SMC1A), mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: SMC1A
• Synonyms: CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC215888).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_006306
• ORF Size: 3699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006306.2, NP_006297.2
• RefSeq Size: 9725
• RefSeq ORF: 3702
• Locus ID: 8243
• Domains: SMC_N, SMC_C, KID
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis
• MW: 143.1 kDa
• Gene Summary: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]