SMC1 (SMC1A) (NM_006306) Human Untagged Clone

CAT#: SC126775

SMC1A (untagged)-Human structural maintenance of chromosomes 1A (SMC1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMC1A
• Synonyms: CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126775 sequence for NM_006306 edited (data generated by NextGen Sequencing)
  ATGGGGTTCCTGAAACTGATTGAGATTGAGAACTTTAAGTCGTACAAGGGTCGACAGATT
  ATCGGACCATTTCAGAGGNNNNNNGCCATCATTGGACCCAATGGCTCTGGTAAGTCAAAT
  CTCATGGATGCCATCAGCTTTGTGCTAGGTGAAAAAACCAGCAACCTGCGGGTAAAGACC
  CTGCGGGACCTGATCCATGGAGCTCCTGTGGGCAAGCCAGCTGCCAACCGGGCCTTTGTC
  AGCATGGTCTACTCTGAGGAGGGTGCTGAGGACCGTACCTTTGCCCGTGTCATTGTAGGA
  GGTTCTTCTGAGTACAAGATCAACAACAAAGTGGTCCAACTACATGAGTACAGTGAGGAA
  TTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTTTTCCAGGGTGCTGTG
  GAATCTATTGCCATGAAGAACCCCAAAGAGAGGACAGCTCTATTTGAAGAGATTAGTCGT
  TCTGGGGAGCTGGCGCAGGAGTATGACAAGCGAAAGAAGGAAATGGTGAAGGCTGAAGAG
  GACACACAGTTTAATTACCATCGCAAGAAAAATATTGCGGCTGAACGCAAGGAAGCAAAG
  CAGGAGAAAGAAGAGGCTGACCGGTACCAGCGCCTGAAGGATGAGGTAGTACGGGCTCAG
  GTACAGCTGCAGCTCTTTAAGCTTTACCATAATGAAGTGGAAATTGAGAAGCTCAACAAG
  GAACTGGCCTCAAAGAACAAGGAGATCGAGAAGGACAAGAAGCGTATGGACAAGGTGGAG
  GATGAACTGAAGGAGAAGAAGAAGGAGCTGGGCAAAATGATGCGGGAGCAGCAGCAGATT
  GAGAAGGAGATCAAGGAGAAGGACTCAGAATTGAACCAGAAGCGGCCTCAGTACATCAAA
  GCCAAGGAGAACACCTCCCACAAAATCAAGAAGCTGGAAGCAGCCAAGAAGTCTCTGCAG
  AATGCTCAGAAGCACTACAAGAAGCGTAAAGGTGACATGGATGAGCTGGAGAAGGAGATG
  CTGTCAGTGGAGAAGGCTCGGCAGGAGTTTGAAGAACGGATGGAAGAAGAGAGTCAGAGT
  CAGGGCAGAGATTTGACGTTGGAGGAGAATCAGGTGAAGAAATACCACCGGTTGAAAGAA
  GAAGCCAGCAAGAGAGCAGCTACCCTGGCCCAGGAGCTGGAGAAATTCAATCGAGACCAG
  AAAGCTGACCAGGACCGTCTGGATCTGGAAGAACGGAAGAAAGTAGAGACAGAGGCCAAG
  ATCAAGCAAAAGCTGCGGGAAATTGAAGAGAATCAGAAGCGGATTGAGAAACTGGAGGAA
  TACATCACCACTAGCAAGCAGTCCCTAGAAGAGCAGAAGAAGCTAGAGGGGGAGCTGACA
  GAGGAGGTGGAGATGGCCAAGCGGCGTATTGATGAAATCAATAAGGAGCTGAACCAGGTG
  ATGGAGCAGCTAGGGGATGCCCGCATCGACCGCCAGGAGAGCAGCCGCCAGCAGCGAAAG
  GCAGAGATAATGGAAAGCATCAAGCGCCTTTACCCTGGCTCTGTGTACGGCCGCCTCATT
  GACCTATGCCAGCCCACACAAAAGAAGTATCAGATTGCTGTAACCAAGGTTTTGGGCAAG
  AACATGGATGCCATTATTGTGGACTCGGAGAAGACAGGCCGGGACTGTATTCAGTATATC
  AAGGAGCAGCGTGGGGAGCCTGAGACCTTCTTGCCTCTTGACTACCTGGAGGTGAAGCCT
  ACAGATGAGAAACTCCGGGAGCTGAAGGGGGCCAAGCTAGTGATTGATGTGATTCGCTAT
  GAGCCACCTCATATCAAAAAGGCCCTGCAGTATGCTTGTGGCAATGCCCTTGTCTGTGAC
  AACGTGGAAGATGCCCGCCGCATTGCCTTTGGAGGCCACCAGCGCCACAAGACAGTGGCA
  CTGGATGGAACCCTATTCCAGAAGTCAGGAGTGATCTCTGGTGGGGCCAGTGACCTGAAG
  GCCAAGGCACGGCGCTGGGATGAGAAAGCAGTAGACAAGTTGAAAGAGAAGAAGGAGCGC
  TTGACAGAGGAGCTGAAAGAGCAGATGAAGGCAAAACGGAAAGAGGCAGAGCTGCGTCAG
  GTGCAGTCTCAGGCCCATGGACTGCAGATGCGGCTCAAGTACTCCCAGAGTGACCTAGAA
  CAGACCAAGACACGACATCTAGCCCTGAATCTGCAGGAAAAATCCAAGCTGGAGAGTGAG
  CTAGCCAACTTTGGGCCTCGCATTAATGATATCAAGAGGATCATTCAGAGCCGAGAGAGG
  GAAATGAAAGACTTGAAGGAGAAGATGAACCAGGTAGAGGATGAGGTGTTTGAAGAGTTT
  TGTCGGGAGATTGGTGTGCGCAACATCCGGGAGTTTGAGGAAGAAAAGGTGAAACGGCAG
  AATGAAATCGCCAAGAAGCGTTTGGAGTTTGAGAATCAGAAGACTCGCTTGGGCATTCAG
  TTGGATTTTGAAAAGAACCAACTGAAGGAGGACCAAGATAAAGTACACATGTGGGAGCAG
  ACAGTGAAAAAAGATGAAAATGAGATAGAAAAGCTCAAAAAGGAGGAACAAAGACACATG
  AAGATCATAGATGAGACCATGGCTCAGCTACAAGACCTGAAGAATCAGCATCTGGCCAAG
  AAGTCGGAAGTGAATGACAAGAATCATGAGATGGAGGAGATTCGTAAGAAACTCGGGGGC
  GCCAACAAGGAAATGACCCATTTACAGAAGGAGGTGACAGCCATTGAGACCAAGCTTGAA
  CAGAAGCGCAGTGACCGTCACAACTTGCTACAGGCCTGTAAGATGCAGGACATTAAGTTG
  CCACTGTCAAAAGGCACCATGGATGATATTAGTCAGGAAGAGGGTAGCTCCCAGGGGGAG
  GACTCAGTGAGTGGTTCACAGAGAATTTCCAGTATCTATGCACGAGAGGCCCTCATTGAG
  ATTGACTACGGTGATCTGTGTGAGGATCTGAAGGATGCCCAGGCTGAGGAAGAGATCAAG
  CAAGAGATGAACACACTGCAGCAGAAGCTGAATGAGCAGCAGAGTGTGCTTCAGCGTATT
  GCCGCCCCCAACATGAAGGCCATGGAAAAGCTGGAAAGTGTCCGAGACAAGTTCCAGGAG
  ACCTCAGATGAGTTTGAAGCAGCCCGAAAGCGAGCAAAGAAGGCCAAGCAGGCATTCGAA
  CAGATCAAGAAGGAGCGCTTTGACCGCTTCAATGCTTGTTTTGAATCTGTGGCTACCAAC
  ATTGATGAGATCTATAAGGCCCTGTCCCGCAATAGCAGTGCCCAGGCATTCCTGGGCCCT
  GAGAACCCTGAAGAGCCCTACTTGGATGGCATCAACTACAACTGTGTGGCTCCTGGGAAA
  CGCTTCCGGCCTATGGACAACTTGTCAGGCGGGGAGAAGACAGTGGCAGCTCTGGCCCTG
  CTCTTTGCCATCCACAGCTACAAGCCAGCCCCCTTCTTCGTCCTGGATGAGATTGATGCT
  GCCTTGGATAACACCAACATTGGCAAGGTGGCAAATTACATCAAGGAGCAGTCGACTTGC
  AACTTCCAGGCCATCGTCATCTCTCTCAAGGAGGAGTTCTACACCAAGGCCGAGAGCCTC
  ATTGGAGTCTATCCTGAGCAAGGGGACTGTGTGATCAGCAAAGTCCTGACCTTCGACCTC
  ACCAAGTACCCAGATGCCAACCCCAACCCCAATGAGCAGTAG
  
  Clone variation with respect to NM_006306.2
  79 t=>n;80 t=>n;81 c=>n;82 a=>n;83 c=>n;84 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006306 unedited
  TTCCCGCGCGGGCTACCTCAGTTCTCGGGCGTACGGCGCGGCCTGTCCTACTGCCGCCGG
  CGCCGCGGCCGTCATGGGGTTCCTGAAACTGATTGAGATTGAGAACTTTAAGTCGTACAA
  GGGTCGACAGATTATCGGACCATTTCAGAGGTTCACCGCCATCATTGGACCCAATGGCTC
  TGGTAAGTCAAATCTCATGGATGCCATCAGCTTTGTGCTAGGTGAAAAAACCAGCAACCT
  GCGGGTAAAGACCCTGCGGGACCTGATCCATGGAGCTCCTGTGGGCAAGCCAGCTGCCAA
  CCGGGCCTTTGTCAGCATGGTCTACTCTGAGGAGGGTGCTGAGGACCGTACCTTTGCCCG
  TGTCATTGTAGGAGGTTCTTCTGAGTACAAGATCAACAACAAAGTGGTCCAACTACATGA
  GTACAGTGAGGAATTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTTTT
  CCAGGGTGCTGTGGAATCTATTGCCATGAAGAACCCCAAAGAGAGGACAGCTCTATTTGA
  AGAGATTAGTCGTTCTGGGGAGCTGGCGCAGGAGTATGACAAGCGAAAGAAGGAAATGGT
  GAAGGCTGAAGAGGACACACAGTTTAATTACCATCGCAAGAAAAATATTGCGGCTGAACG
  CAAGGAAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006306 unedited
  NGGGCGTACTGTGNACCGCGGCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTNTCTAAAACAGGGTCTCCAATATCCTTTATTATTTTGCAAACATACTCACATGCACAC
  ATGCGGATACATACATACACACATACATACCCACACACACAGTGGGCAAAAGGCCCAAGG
  GGCCCACGATTATGGGTGATGAGGGGGAGGAAGGGGGCCTTTCTTTTTTTTTTGTTAAAT
  CACCACCCCTTTCTATTATCTTATACTATCTGCCCCCCTTGGACACCTCTTTTGCCCCTC
  ACTAAACCTTTCCTCTCCCACCATATCTCTCCCCTACACTTTACATGTCTTCACTTCTCG
  CTGATTCTTTATTCATACCATCATACTCACCTTAGTATCTCCATAATAATCTCATTAGCG
  CTGCCGTCCACTTAGTCTCTGCCGCACAGCTGACTCCATCTTCTTTCTGCCTTGTCTCAT
  ATAGACTAACCTTAACCAGCTCAGGATTCGAACCTCGCAGACCAGCCAATATATTAGAGT
  ACGAGGAGAGGAGCGAAGTCCTAGTTAGAAGACCATATGTACGCGAGTTGGCCAGATTAG
  CTACAGACCTTAAGATTCCGCAGAAATTAGTATGCCGCCAGTAGATAGCATAGGAACAAT
  TCACCTTAGTCCTCACATATCGATCAATATATTCACCCGCTTTAACGCGATATCTCAGTA
  AGTGCCGACACCCGATTATGTGAATCATGAGAGATCGGATGCGAGTAATTCAACGATTTA
  TTGGAATTCAAGACATGATACACACAGACACATTANCATGATATAGACCTACAAGTATGC
  AAGCACAACAATGGCCGCACATATATAGCAGAGATAACTATAAGGATATCAGACGAGGGA
  CAATGCTCACTGTAGACGACGTAAGTAATTAAAGAGACAATGTAATAGTAACATTAGAGT
  GACACATTCAAAATAGCACACGCGGAGCACACACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006306
• ORF Size: 3702 bp
• Insert Size: 4350
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006306.2, NP_006297.2
• RefSeq Size: 9725
• RefSeq ORF: 3702
• Locus ID: 8243
• Domains: SMC_N, SMC_C, KID
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis
• Gene Summary: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.