LAMA3 (NM_000227) Human Tagged ORF Clone
CAT#: RC215891L3
- LentiORF®
Lenti ORF clone of Human laminin, alpha 3 (LAMA3), transcript variant 2, Myc-DDK-tagged
"NM_000227" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LAMA3 |
Synonyms | BM600; E170; LAMNA; LOCS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC215891).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000227 |
ORF Size | 5172 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000227.2, NP_000218.2 |
RefSeq Size | 5601 bp |
RefSeq ORF | 5175 bp |
Locus ID | 3909 |
Cytogenetics | 18q11.2 |
Domains | LamG, EGF_Lam |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
MW | 190.3 kDa |
Gene Summary | 'The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108826 | LAMA3 (untagged)-Human laminin, alpha 3 (LAMA3), transcript variant 2 |
USD 3,490.00 |
|
RC215891 | LAMA3 (Myc-DDK-tagged)-Human laminin, alpha 3 (LAMA3), transcript variant 2 |
USD 1,810.00 |
|
RG215891 | LAMA3 (GFP-tagged) - Human laminin, alpha 3 (LAMA3), transcript variant 2 |
USD 1,990.00 |
|
RC215891L1 | Lenti ORF clone of Human laminin, alpha 3 (LAMA3), transcript variant 2, Myc-DDK-tagged |
USD 2,484.00 |
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