LAMA3 (NM_000227) Human Untagged Clone

CAT#: SC108826

LAMA3 (untagged)-Human laminin, alpha 3 (LAMA3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAMA3
• Synonyms: BM600; E170; LAMNA; LOCS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108826 sequence for NM_000227 edited (data generated by NextGen Sequencing)
  ATGCCTCCAGCAGTGAGGCGGTCAGCCTGCAGCATGGGATGGCTGTGGATCTTTGGGGCA
  GCCCTGGGGCAGTGTCTGGGCTACAGTTCACAGCAGCAAAGGGTGCCATTTCTTCAGCCT
  CCCGGTCAAAGTCAACTGCAAGCGAGTTATGTGGAGTTTAGACCCAGCCAGGGTTGTAGC
  CCTGGATACTATCGGGATCATAAAGGCTTGTATACCGGACGGTGTGTTCCCTGCAATTGC
  AACGGACATTCAAATCAATGCCAGGATGGCTCAGGCATATGTGTTAACTGTCAGCACAAC
  ACCGCGGGAGAGCACTGTGAACGCTGCCAGGAGGGCTACTATGGCAACGCCGTCCACGGA
  TCCTGCAGGGCCTGCCCATGTCCTCACACTAACAGCTTTGCCACTGGCTGTGTGGTGAAT
  GGGGGAGACGTGCGGTGCTCCTGCAAAGCTGGGTACACAGGAACACAGTGTGAAAGGTGT
  GCACCGGGATATTTYGGGAATCCCCAGAAATTCGGAGGTAGCTGCCAACCATGCAGTTGT
  AACAGCAATGGCCAGCTGGGCAGCTGTCATCCCCTGACTGGAGACTGCATAAACCAAGAA
  CCCAAAGATAGCAGCCCTGCAGAAGAATGTGATGATTGCGACAGCTGTGTGATGACCCTC
  CTGAACGACCTGGCCACCATGGGCGAGCAGCTCCGCCTGGTCAAGTCTCAGCTGCAGGGC
  CTGAGTGCCAGCGCAGGGCTTCTGGAGCAGATGAGGCACATGGAGACCCAGGCCAAGGAC
  CTGAGGAATCAGTTGCTCAACTACCGTTCTGCCATTTCAAATCATGGATCAAAAATAGAA
  GGCCTGGAAAGAGAACTGACTGATTTGAATCAAGAATTTGAGACTTTGCAAGAAAAGGCT
  CAAGTAAATTCCAGAAAAGCACAAACATTAAACAACAATGTTAATCGGGCAACACAAAGC
  GCAAAAGAACTGGATGTGAAGATTAAAAATGTCATCCGGAATGTGCACATTCTTTTAAAG
  CAGATCTCTGGGACAGATGGAGAGGGAAACAACGTGCCTTCAGGTGACTTTTCCAGAGAG
  TGGGCTGAAGCCCAGCGCATGATGAGGGAACTGCGGAACAGGAACTTTGGAAAGCACCTC
  AGAGAAGCAGAAGCTGATAAAAGGGAGTCGCAGCTCTTGCTGAACCGGATAAGGACCTGG
  CAGAAAACCCACCAGGGGGAGAACAATGGGCTTGCTAACAGTATCCGGGATTCTTTAAAT
  GAATACGAAGCCAAACTCAGTGACCTTCGTGCTCGGCTGCAGGAGGCAGCTGCCCAAGCC
  AAGCAGGCAAATGGCTTGAACCAAGAAAACGAGAGAGCTTTGGGAGCCATTCAGAGACAA
  GTGAAAGAAATAAATTCCCTGCAGAGTGATTTCACCAAGTATCTAACCACTGCAGACTCA
  TCTTTGTTGCAAACCAACATTGCGCTGCAGCTGATGGAGAAAAGCCAGAAGGAATATGAA
  AAATTAGCTGCCAGTTTAAATGAAGCAAGACAAGAACTAAGTGACAAAGTAAGAGAACTT
  TCCAGATCTGCTGGCAAAACATCCCTTGTGGAGGAGGCAGAAAAGCACGCGCGGTCCTTA
  CAAGAGCTGGCAAAGCAGCTGGAAGAGATCAAGAGAAACGCCAGCGGGGATGAGCTGGTG
  CGCTGTGCTGTGGATGCCGCCACCGCCTACGAGAACATCCTCAATGCCATCAAAGCGGCC
  GAGGACGCAGCCAACAGGGCTGCCAGTGCATCTGAATCTGCCCTCCAGACAGTGATAAAG
  GAAGATCTGCCAAGAAAAGCTAAAACCCTGAGTTCCAACAGTGATAAACTGTTAAATGAA
  GCCAAGATGACACAAAAGAAGCTAAAGCAAGAAGTCAGTCCAGCTCTCAACAACCTACAG
  CAAACCCTGAATATTGTGACAGTTCAGAAAGAAGTGATAGACACCAATCTCACAACTCTC
  CGAGATGGTCTTCATGGGATACAGAGAGGTGATATTGATGCTATGATCAGTAGTGCAAAG
  AGCATGGTCAGAAAGGCCAACGACATCACAGATGAGGTTCTGGATGGGCTCAACCCCATC
  CAGACAGATGTGGAAAGAATTAAGGACACCTATGGGAGGACACAGAACGAAGACTTCAAA
  AAGGCTCTGACTGATGCAGATAACTCGGTGAATAAGTTAACCAACAAACTACCTGATCTT
  TGGCGCAAGATTGAAAGTATCAACCAACAGCTGTTGCCCTTGGGAAACATCTCTGACAAC
  ATGGACAGAATACGAGAACTAATTCAGCAGGCCAGAGATGCTGCCAGTAAGGTTGCTGTC
  CCCATGAGGTTCAATGGTAAATCTGGAGTCGAAGTCCGACTGCCAAATGACCTGGAAGAT
  TTGAAAGGATATACATCTCTGTCCTTGTTTCTCCAAAGGCCCAACTCAAGAGAAAATGGG
  GGTACTGAGAATATGTTTGTGATGTACCTTGGAAATAAAGATGCCTCCCGGGACTACATC
  GGCATGGCAGTTGTGGATGGCCAGCTCACCTGTGTCTACAACCTGGGGGACCGTGAGGCT
  GAACTCCAAGTGGACCAGATCTTGACCAAGAGTGAGACTAAGGAGGCAGTTATGGATCGG
  GTGAAATTTCAGAGAATTTATCAGTTTGCAAGGCTTAATTACACCAAAGGAGCCACATCC
  AGTAAACCAGAAACACCCGGAGTCTATGACATGGATGGTAGAAATAGCAATACACTCCTT
  AATTTGGATCCTGAAAATGTTGTATTTTATGTTGGAGGTTACCCACCTGATTTTAAACTT
  CCCAGTCGACTAAGTTTCCCTCCATACAAAGGTTGTATTGAATTAGATGACCTCAATGAA
  AATGTTCTGAGCTTGTACAACTTCAAAAAAACATTCAATCTCAACACAACTGAAGTGGAG
  CCTTGTAGAAGGAGGAAGGAAGAGTCAGACAAAAATTATTTTGAAGGTACGGGCTATGCT
  CGAGTTCCAACTCAACCACATGCTCCCATCCCAACCTTTGGACAGACAATTCAGACCACC
  GTGGATAGAGGCTTGCTGTTCTTTGCAGAAAACGGGGATCGCTTCATATCTCTAAATATA
  GAAGATGGCAAGCTCATGGTGAGATACAAACTGAATTCAGAGCTACCAAAAGAGAGAGGA
  GTTGGAGACGCCATAAACAACGGCAGAGACCATTCGATTCAGATCAAAATTGGAAAACTC
  CAAAAGCGTATGTGGATAAATGTGGACGTTCAAAACACTATAATTGATGGTGAAGTATTT
  GATTTCAGCACATATTATCTGGGAGGAATTCCAATTGCAATCAGGGAAAGATTTAACATT
  TCTACGCCTGCTTTCCGAGGCTGCATGAAAAATTTGAAGAAAACCAGTGGTGTCGTTAGA
  TTGAATGATACTGTGGGAGTAACCAAAAAGTGCTCGGAAGACTGGAAGCTTGTGCGATCT
  GCCTCATTCTCCAGAGGAGGACAATTGAGTTTCACTGATTTGGGCTTACCACCTACTGAC
  CACCTCCAGGCCTCATTTGGATTTCAGACCTTTCAACCCAGTGGCATATTATTAGATCAT
  CAGACATGGACAAGGAACCTGCAGGTCACTCTGGAAGATGGTTACATTGAATTGAGCACC
  AGCGATAGCGGCGGCCCAATTTTTAAATCTCCACAGACGTATATGGATGGTTTACTGCAT
  TATGTATCTGTAATAAGCGACAACTCTGGACTACGGCTTCTCATCGATGACCAGCTTCTG
  AGAAATAGCAAAAGGCTAAAACACATTTCAAGTTCCCGGCAGTCTCTGCGTCTGGGCGGG
  AGCAATTTTGAGGGTTGTATTAGCAATGTTTTTGTCCAGAGGTTATCACTGAGTCCTGAA
  GTCCTAGATTTGACCAGTAACTCTCTCAAGAGAGATGTGTCCCTGGGAGGCTGCAGTTTA
  AACAAACCACCTTTTCTAATGTTGCTTAAAGGTTCTACCAGGTTTAACAAGACCAAGACT
  TTTCGTATCAACCAGCTGTTGCAGGACACACCAGTGGCCTCCCCAAGGAGCGTGAAGGTG
  TGGCAAGATGCTTGCTCACCACTTCCCAAGACCCAGGCCAATCATGGAGCCCTCCAGTTT
  GGGGACATTCCCACCAGCCACTTGCTATTCAAGCTTCCTCAGGAGCTGCTGAAACCCAGG
  TCACAGTTTGCTGTGGACATGCAGACAACATCCTCCAGAGGACTGGTGTTTCACACGGGC
  ACTAAGAACTCCTTTATGGCTCTTTATCTTTCAAAAGGACGTCTGGTCTTTGCACTGGGG
  ACAGATGGGAAAAAATTGAGGATCAAAAGCAAGGAGAAATGCAATGATGGGAAATGGCAC
  ACGGTGGTGTTTGGCCATGATGGGGAAAAGGGGCGCTTGGTTGTGGATGGACTGAGGGCC
  CGGGAGGGAAGTTTGCCTGGAAACTCCACCATCAGCATCAGAGCGCCAGTTTACCTGGGA
  TCACCTCCATCAGGGAAACCAAAGARCCTCCCCACAAACAGCTTTGTGGGATGCCTGAAG
  AACTTTCAGCTGGATTCAAAACCCTTGTATACCCCTTCTTCAAGCTTCGGGGTGTCTTCC
  TGCTTGGGTGGTCCTTTGGAGAAAGGCATTTATTTCTCTGAAGAAGGAGGTCATGTCGTC
  TTGGCTCACTCTGTATTGTTGGGGCCAGAATTTAAGCTTGTTTTCAGCATCCGCCCAAGA
  AGTCTCACTGGGATCCTAATACACATCGGAAGTCAGCCCGGGAAGCACTTATGTGTTTAC
  CTGGAGGCAGGAAAGGTCACGGCCTCTATGGACAGTGGGGCAGGTGGGACCTCAACGTCG
  GTCACACCAAAGCAGTCTCTGTGTGATGGACAGTGGCACTCGGTGGCAGTCACCATAAAA
  CAACACATCCTGCACCTGGAACTGGACACAGACAGTAGCTACACAGCTGGACAGATCCCC
  TTCCCACCTGCCAGCACTCAAGAGCCACTACACCTTGGAGGTGCTCCAGCCAATTTGACG
  ACACTGAGGATCCCTGTGTGGAAATCATTCTTTGGCTGTCTGAGGAATATTCATGTCAAT
  CACATCCCTGTCCCTGTCACTGAAGCCTTGGAAGTCCAGGGGCCTGTCAGTCTGAATGGT
  TGTCCTGACCAGTAA
  
  Clone variation with respect to NM_000227.3
  495 c=>y;3673 a=>g;4526 g=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000227 unedited
  GGAACCGGGATGCCTCCAGCAGTGAGGCGGTCAGCCTGCAGCATGGGATGGCTGTGGATC
  TTTGGGGCAGCCCTGGGGCAGTGTCTGGGCTACAGTTCACAGCAGCAAAGGGTGCCATTT
  CTTCAGCCTCCCGGTCAAAGTCAACTGCAAGCGAGTTATGTGGAGTTTAGACCCAGCCAG
  GGTTGTAGCCCTGGATACTATCGGGATCATAAAGGCTTGTATACCGGACGGTGTGTTCCC
  TGCAATTGCAACGGACATTCAAATCAATGCCAGGATGGCTCAGGCATATGTGTTAACTGT
  CAGCACAACACCGCGGGAGAGCACTGTGAACGCTGCCAGGAGGGCTACTATGGCAACGCC
  GTCCACGGATCCTGCAGGGCCTGCCCATGTCCTCACACTAACAGCTTTGCCACTGGCTGT
  GTGGTGAATGGGGGAGACGTGCGGTGCTCCTGCAAAGCTGGGTACACCGGAACACAGTGT
  GAAAGGTGTGCCACCGGAATATTTTCGGGAATCCCCACAAATTCGGAGGTACCTGCCAAC
  CATGCCAGTTGGCACAGCCAATGGCCACCTGGGCAGCTGTCCTCCCCTTGACTGCAAACT
  GCCTTAACCCAAGATCCCAAAGCATAGCACTCCTGCTCAAAAAATGCGCACACTTGTAAC
  CGCTGAGTGATGACCCTCCTGAACCAACCTGCCCAACATGGGCCACTACCCCTTCCTGCC
  AGCCTCAACCTACGGCCCTACGCTCTCCCCTAGGCCCCGCCCCAATGAGCCTAGCAAACC
  CTCCCCAGCACCTGGCGACCCTTCATCATCCCCACCCTCCTACCAAATCTCCCCCCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000227
• Insert Size: 5200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000227.2, NP_000218.2
• RefSeq Size: 5601 bp
• RefSeq ORF: 5175 bp
• Locus ID: 3909
• Cytogenetics: 18q11.2
• Domains: LamG, EGF_Lam
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (2) has a distinct 5' UTR, lacks exons in the 5' coding region, and uses an alternate start codon compared to variant 1. The encoded isoform (2, also known as alpha3a) has a distinct and shorter N-terminus than isoform 1. Variants 2 and 4 encode 'a' isoforms. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.