RP105 (CD180) (NM_005582) Human Tagged ORF Clone

CAT#: RC215954

CD180 (Myc-DDK-tagged)-Human CD180 molecule (CD180)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD180
• Synonyms: LY64; Ly78; RP105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215954 representing NM_005582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTTGACGTCAGCTGCTTCTTTTGGGTGGTGCTGTTTTCTGCCGGCTGTAAAGTCATCACCTCCT
  GGGATCAGATGTGCATTGAGAAAGAAGCCAACAAAACATATAACTGTGAAAATTTAGGTCTCAGTGAAAT
  CCCTGACACTCTACCAAACACAACAGAATTTTTGGAATTCAGCTTTAATTTTTTGCCTACAATTCACAAT
  AGAACCTTCAGCAGACTCATGAATCTTACCTTTTTGGATTTAACTAGGTGCCAGATTAACTGGATACATG
  AAGACACTTTTCAAAGCCATCATCAATTAAGCACACTTGTGTTAACTGGAAATCCCCTGATATTCATGGC
  AGAAACATCGCTTAATGGGCCCAAGTCACTGAAGCATCTTTTCTTAATCCAAACGGGAATATCCAATCTC
  GAGTTTATTCCAGTGCACAATCTGGAAAACTTGGAAAGCTTGTATCTTGGAAGCAACCATATTTCCTCCA
  TTAAGTTCCCCAAAGACTTCCCAGCACGGAATCTGAAAGTACTGGATTTTCAGAATAATGCTATACACTA
  CATCTCTAGAGAAGACATGAGGTCTCTGGAGCAGGCCATCAACCTAAGCCTGAACTTCAATGGCAATAAT
  GTTAAAGGTATTGAGCTTGGGGCTTTTGATTCAACGATCTTCCAAAGTTTGAACTTTGGAGGAACTCCAA
  ATTTGTCTGTTATATTCAATGGTCTGCAGAACTCTACTACTCAGTCTCTCTGGCTGGGAACATTTGAGGA
  CATTGATGACGAAGATATTAGTTCAGCCATGCTCAAGGGACTCTGTGAAATGTCTGTTGAGAGCCTCAAC
  CTGCAGGAACACCGCTTCTCTGACATCTCATCCACCACATTTCAGTGCTTCACCCAACTCCAAGAATTGG
  ATCTGACAGCAACTCACTTGAAAGGGTTACCCTCTGGGATGAAGGGTCTGAACTTGCTCAAGAAATTAGT
  TCTCAGTGTAAATCATTTCGATCAATTGTGTCAAATCAGTGCTGCCAATTTCCCCTCCCTTACACACCTC
  TACATCAGAGGCAACGTGAAGAAACTTCACCTTGGTGTTGGCTGCTTGGAGAAACTAGGAAACCTTCAGA
  CACTTGATTTAAGCCATAATGACATAGAGGCTTCTGACTGCTGCAGTCTGCAACTCAAAAACCTGTCCCA
  CTTGCAAACCTTAAACCTGAGCCACAATGAGCCTCTTGGTCTCCAGAGTCAGGCATTCAAAGAATGTCCT
  CAGCTAGAACTCCTCGATTTGGCATTTACCCGCTTACACATTAATGCTCCACAAAGTCCCTTCCAAAACC
  TCCATTTCCTTCAGGTTCTGAATCTCACTTACTGCTTCCTTGATACCAGCAATCAGCATCTTCTAGCAGG
  CCTACCAGTTCTCCGGCATCTCAACTTAAAAGGGAATCACTTTCAAGATGGGACTATCACGAAGACCAAC
  CTACTTCAGACCGTGGGCAGCTTGGAGGTTCTGATTTTGTCCTCTTGTGGTCTCCTCTCTATAGACCAGC
  AAGCATTCCACAGCTTGGGAAAAATGAGCCATGTAGACTTAAGCCACAACAGCCTGACATGCGACAGCAT
  TGATTCTCTTAGCCATCTTAAGGGAATCTACCTCAATCTGGCTGCCAACAGCATTAACATCATCTCACCC
  CGTCTCCTCCCTATCTTGTCCCAGCAGAGCACCATTAATTTAAGTCATAACCCCCTGGACTGCACTTGCT
  CGAATATTCATTTCTTAACATGGTACAAAGAAAACCTGCACAAACTTGAAGGCTCGGAGGAGACCACGTG
  TGCAAACCCGCCATCTCTAAGGGGAGTTAAGCTATCTGATGTCAAGCTTTCCTGTGGGATTACAGCCATA
  GGCATTTTCTTTCTCATAGTATTTCTATTATTGTTGGCTATTCTGCTATTTTTTGCAGTTAAATACCTTC
  TCAGGTGGAAATACCAACACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215954 representing NM_005582
  Red=Cloning site Green=Tags(s)
  
  MAFDVSCFFWVVLFSAGCKVITSWDQMCIEKEANKTYNCENLGLSEIPDTLPNTTEFLEFSFNFLPTIHN
  RTFSRLMNLTFLDLTRCQINWIHEDTFQSHHQLSTLVLTGNPLIFMAETSLNGPKSLKHLFLIQTGISNL
  EFIPVHNLENLESLYLGSNHISSIKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNN
  VKGIELGAFDSTIFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLN
  LQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHL
  YIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCSLQLKNLSHLQTLNLSHNEPLGLQSQAFKECP
  QLELLDLAFTRLHINAPQSPFQNLHFLQVLNLTYCFLDTSNQHLLAGLPVLRHLNLKGNHFQDGTITKTN
  LLQTVGSLEVLILSSCGLLSIDQQAFHSLGKMSHVDLSHNSLTCDSIDSLSHLKGIYLNLAANSINIISP
  RLLPILSQQSTINLSHNPLDCTCSNIHFLTWYKENLHKLEGSEETTCANPPSLRGVKLSDVKLSCGITAI
  GIFFLIVFLLLLAILLFFAVKYLLRWKYQHI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005582
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005582.1, NM_005582.2, NP_005573.1, NP_005573.2
• RefSeq Size: 2725 bp
• RefSeq ORF: 1986 bp
• Locus ID: 4064
• Cytogenetics: 5q12.3
• Domains: LRRCT, LRR, LRR_TYP
• Protein Families: Druggable Genome, Transmembrane
• MW: 74.6 kDa
• Gene Summary: 'CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]'