RP105 (CD180) (NM_005582) Human Untagged Clone

CAT#: SC116668

CD180 (untagged)-Human CD180 molecule (CD180)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD180
• Synonyms: LY64; Ly78; RP105
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005582, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTTTGACGTCAGCTGCTTCTTTTGGGTGGTGCTGTTTTCTGCCGGCTGTAAAGTCATCACCTCCT
  GGGATCAGATGTGCATTGAGAAAGAAGCCAACAAAACATATAACTGTGAAAATTTAGGTCTCAGTGAAAT
  CCCTGACACTCTACCAAACACAACAGAATTTTTGGAATTCAGCTTTAATTTTTTGCCTACAATTCACAAT
  AGAACCTTCAGCAGACTCATGAATCTTACCTTTTTGGATTTAACTAGGTGCCAGATTAACTGGATACATG
  AAGACACTTTTCAAAGCCATCATCAATTAAGCACACTTGTGTTAACTGGAAATCCCCTGATATTCATGGC
  AGAAACATCGCTTAATGGGCCCAAGTCACTGAAGCATCTTTTCTTAATCCAAACGGGAATATCCAATCTC
  GAGTTTATTCCAGTGCACAATCTGGAAAACTTGGAAAGCTTGTATCTTGGAAGCAACCATATTTCCTCCA
  TTAAGTTCCCCAAAGACTTCCCAGCACGGAATCTGAAAGTACTGGATTTTCAGAATAATGCTATACACTA
  CATCTCTAGAGAAGACATGAGGTCTCTGGAGCAGGCCATCAACCTAAGCCTGAACTTCAATGGCAATAAT
  GTTAAAGGTATTGAGCTTGGGGCTTTTGATTCAACGATCTTCCAAAGTTTGAACTTTGGAGGAACTCCAA
  ATTTGTCTGTTATATTCAATGGTCTGCAGAACTCTACTACTCAGTCTCTCTGGCTGGGAACATTTGAGGA
  CATTGATGACGAAGATATTAGTTCAGCCATGCTCAAGGGACTCTGTGAAATGTCTGTTGAGAGCCTCAAC
  CTGCAGGAACACCGCTTCTCTGACATCTCATCCACCACATTTCAGTGCTTCACCCAACTCCAAGAATTGG
  ATCTGACAGCAACTCACTTGAAAGGGTTACCCTCTGGGATGAAGGGTCTGAACTTGCTCAAGAAATTAGT
  TCTCAGTGTAAATCATTTCGATCAATTGTGTCAAATCAGTGCTGCCAATTTCCCCTCCCTTACACACCTC
  TACATCAGAGGCAACGTGAAGAAACTTCACCTTGGTGTTGGCTGCTTGGAGAAACTAGGAAACCTTCAGA
  CACTTGATTTAAGCCATAATGACATAGAGGCTTCTGACTGCTGCAGTCTGCAACTCAAAAACCTGTCCCA
  CTTGCAAACCTTAAACCTGAGCCACAATGAGCCTCTTGGTCTCCAGAGTCAGGCATTCAAAGAATGTCCT
  CAGCTAGAACTCCTCGATTTGGCATTTACCCGCTTACACATTAATGCTCCACAAAGTCCCTTCCAAAACC
  TCCATTTCCTTCAGGTTCTGAATCTCACTTACTGCTTCCTTGATACCAGCAATCAGCATCTTCTAGCAGG
  CCTACCAGTTCTCCGGCATCTCAACTTAAAAGGGAATCACTTTCAAGATGGGACTATCACGAAGACCAAC
  CTACTTCAGACCGTGGGCAGCTTGGAGGTTCTGATTTTGTCCTCTTGTGGTCTCCTCTCTATAGACCAGC
  AAGCATTCCACAGCTTGGGAAAAATGAGCCATGTAGACTTAAGCCACAACAGCCTGACATGCGACAGCAT
  TGATTCTCTTAGCCATCTTAAGGGAATCTACCTCAATCTGGCTGCCAACAGCATTAACATCATCTCACCC
  CGTCTCCTCCCTATCTTGTCCCAGCAGAGCACCATTAATTTAAGTCATAACCCCCTGGACTGCACTTGCT
  CGAATATTCATTTCTTAACATGGTACAAAGAAAACCTGCACAAACTTGAAGGCTCGGAGGAGACCACGTG
  TGCAAACCCGCCATCTCTAAGGGGAGTTAAGCTATCTGATGTCAAGCTTTCCTGTGGGATTACAGCCATA
  GGCATTTTCTTTCTCATAGTATTTCTATTATTGTTGGCTATTCTGCTATTTTTTGCAGTTAAATACCTTC
  TCAGGTGGAAATACCAACACATTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005582 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGTAGGTAAACCCACCAAGCAATC
  CTAGCCTGTGATGGCGTTTGACGTCAGCTGCTTCTTTTGGGTGGTGCTGTTTTCTGCCGG
  CTGTAAAGTCATCACCTCCTGGGATCAGATGTGCATTGAGAAAGAAGCCAACAAAACATA
  TAACTGTGAAAATTTAGGTCTCAGTGAAATCCCTGACACTCTACCAAACACAACAGAATT
  TTTGGAATTCAGCTTTAATTTTTTGCCTACAATTCACAATAGAACCTTCAGCAGACTCAT
  GAATCTTACCTTTTTGGATTTAACTAGGTGCCAGATTAACTGGATACATGAAGACACTTT
  TCAAAGCCATCATCAATTAAGCACACTTGTGTTAACTGGAAATCCCCTGATATTCATGGC
  AGAAACATCGCTTAATGGGCCCAAGTCACTGAAGCATCTTTTCTTAATCCAAACGGGAAT
  ATCCAATCTCGAGTTTATTCCAGTGCACAATCTGGAAAACTTGGAAAGCTTGTATCTTGG
  AAGCAACCATATTTCCTCCATTAAGTTCCCCAAAGACTTCCCAGCACGGAATCTGAAAGT
  ACTGGATTTTCAGAATAATGCTATACACTACATCTCTAGAGAGACATGANGTCTCTGGAG
  CAGGCCATCAACCTAAGCCTGAACTTCATGGCAATATGTTAAAGGTATTGAGCTTGGGGC
  TTTTGATTCAACGGTCTTNCCAAAGTTGAACTNTGNAGGACTNNCAATTNGTCTGTTATA
  TTCAATGGTCTGCAGAACTCTACTACTCAGTCTCTCTGGCTGGGAACATTTGAGGACATT
  GATGACAAGNAATATANNNTCANCATGCTCANGGGACTCTGTGAAATGTCTGTNAGAGCC
  TCAACTGCAGAACACGCTTTCTGACATTATCACACATTTAGTGCTTCCCACTCAGAATNT
  GATCGACGCACTACTGAAGGNTACCTTGGAGTGAAGGCTGACTGCACAAATATNTCAGGA
  TACTTCAACATGGCAACAGCGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005582 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTCTCAGCCTTTTCTTTTA
  AATTTATTATGCTTTTCTTTTCTTTTTTCATTTAAAATAAGGATTGAGACTTTGTCCCCA
  AGTAAGTTTCTCAAATGGCTACATTACTGAAAACCCAGACCCAAGCACAATGCTGTAGTG
  GGGGTGGGTAGTGGGCTGGCTTCAGCTCTGACAAATTGTGGGGGCTGATGATCTCCTTTC
  AGGGGCGGGGTGTGTGGTGCTGGGAGGGATGTCGGTGAGTAAAAGGGGAATGGCCTCCCT
  GTGGGAGACTCCGGGGCCGGCAGTCCCTGCCCAGTCCCTCCCTCTGCTTCCTCCCACTCT
  TCCACATGCGGAAAGGGCTCTGTGCCTCTCACAGCAGCATCTGACCCTCTGGACTGAGTC
  ATTCGGTGTACTTGCCTAAAAGGTTCTTTAGCTCTGGGACTCACAGGAGTAAGAAGCTCA
  GAAAAGCACAGTGAGTCCCTGCCCAGTTCCAGGAAGCAATCTGAAAAGTCTGGTCTGGTC
  ACCAGCAGATGACAGTTCTTTCACTTAGAGCAATTTTGCTAAGCACACTTATTTGCTTTC
  TCTGGAAACCTTCAGCACTAAATGTGTTGGTATTTCCACCTGAGAAGGTATTTAACTGCA
  AAAAATAGCAGAATAGCCAACAATAATAGAAATACTATGAGAAAGAAAATGCCTATGGCT
  GTAATCCCACAGGAAAGCTTGACATCAGATAGCTTAACTCCCCTTAGAGATGGCGGGGTT
  TGCACACGTGGTCTCCTCCGAGCCTTCAAGTTGTGCAGTTTTCTTTGTACATGTTAAGAA
  ATGATATTCGGCAGTGCCGTCCAGGGGTTATGACTAAAAAAAGGGGCTCTGGTGGCACAC
  AAAGGAAGAAACAGGGTGAAAGATGTTATGCTGTTGCACCACATTGAGGAAATCCCTTAA
  AAGCCTACAAATCAAGCCTGCCCTGTACGCTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005582
• Insert Size: 2670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005582.1, NP_005573.1
• RefSeq Size: 2725 bp
• RefSeq ORF: 1986 bp
• Locus ID: 4064
• Cytogenetics: 5q12.3
• Domains: LRRCT, LRR, LRR_TYP
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]'