NMNAT2 (NM_015039) Human Tagged ORF Clone

CAT#: RC216259

NMNAT2 (Myc-DDK-tagged)-Human nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414841) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216259 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NMNAT2
• Synonyms: C1orf15; PNAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216259 representing NM_015039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATCACCAAAGGGC
  ACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGGAT
  TGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAGCCGGCACCGTCTCATCATGTGT
  CAGCTGGCCGTCCAGAATTCTGATTGGATCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGA
  CGACCTGCAGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGT
  CAACACACCTTCCATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAAC
  AGCAACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGATCT
  GCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATGCCAATCTGGGCACGGTGATGCG
  GTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCA
  GGGCTCTGGAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGGTGCCCCGGGATG
  CAGCCGACACAGACCGAATCATGAATCACTCCTCAATACTCCGCAAATACAAAAACAACATCATGGTGGT
  GAAGGATGACATCAACCATCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATGGG
  GACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATCCTCAAAAGCCAGCTGTACATCA
  ATGCCTCCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216259 representing NM_015039
  Red=Cloning site Green=Tags(s)
  
  MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMC
  QLAVQNSDWIRVDPWECYQDTWQTTCSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPIYQN
  SNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCGSDLLESFCIP
  GLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHG
  DGHVVDYLSQPVIDYILKSQLYINASG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015039
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015039.1, NM_015039.2, NM_015039.3, NP_055854.1
• RefSeq Size: 5680
• RefSeq ORF: 924
• Locus ID: 23057
• Domains: CTP_transf_2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• MW: 34.3 kDa
• Gene Summary: This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]