NMNAT2 (NM_015039) Human Tagged ORF Clone

CAT#: RG216259

NMNAT2 (GFP-tagged) - Human nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NMNAT2
• Synonyms: C1orf15; PNAT2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216259 representing NM_015039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATCACCAAAGGGC
  ACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGGAT
  TGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAGCCGGCACCGTCTCATCATGTGT
  CAGCTGGCCGTCCAGAATTCTGATTGGATCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGA
  CGACCTGCAGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGT
  CAACACACCTTCCATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAAC
  AGCAACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGATCT
  GCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATGCCAATCTGGGCACGGTGATGCG
  GTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCA
  GGGCTCTGGAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGGTGCCCCGGGATG
  CAGCCGACACAGACCGAATCATGAATCACTCCTCAATACTCCGCAAATACAAAAACAACATCATGGTGGT
  GAAGGATGACATCAACCATCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATGGG
  GACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATCCTCAAAAGCCAGCTGTACATCA
  ATGCCTCCGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216259 representing NM_015039
  Red=Cloning site Green=Tags(s)
  
  MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMC
  QLAVQNSDWIRVDPWECYQDTWQTTCSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPIYQN
  SNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCGSDLLESFCIP
  GLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHG
  DGHVVDYLSQPVIDYILKSQLYINASG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015039
• ORF Size: 921 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015039.2, NP_055854.1
• RefSeq Size: 5680
• RefSeq ORF: 924
• Locus ID: 23057
• Domains: CTP_transf_2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]