DIAPH1 (NM_005219) Human Tagged ORF Clone

CAT#: RC216318

DIAPH1 (Myc-DDK-tagged)-Human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417437) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216318 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DIAPH1 protein (Cat# TP316318). The protein was produced from HEK293T cells transfected with DIAPH1 cDNA clone (Cat# RC216318) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DIAPH1
• Synonyms: DFNA1; DIA1; DRF1; hDIA1; LFHL1; SCBMS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216318 representing NM_005219
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCGGCGGGAGCCTGGGGCCCGGCCGCGGGACCCGGGACAAGAAGAAGGGCCGGAGCCCAG
  ATGAGCTGCCCTCGGCGGGCGGCGACGGCGGCAAATCTAAGAAATTTACTCTGAAGCGGCTCATGGCAGA
  TGAGCTGGAGAGATTTACCAGCATGAGAATTAAGAAGGAGAAGGAAAAGCCCAATTCTGCTCATAGAAAT
  TCTTCTGCATCATATGGGGATGATCCCACAGCACAGTCATTGCAAGATGTTTCAGATGAACAAGTGCTGG
  TTCTCTTTGAACAGATGCTGCTGGATATGAACCTGAATGAGGAGAAACAGCAACCTTTGAGGGAGAAGGA
  CATCATCATCAAGAGGGAGATGGTGTCCCAATACTTGTACACCTCCAAGGCTGGCATGAGCCAGAAGGAG
  AGCTCTAAGTCTGCCATGATGTATATTCAGGAGTTGAGGTCAGGCTTGCGGGATATGCCTCTGCTCAGCT
  GCCTGGAGTCCCTTCGTGTGTCTCTCAACAACAACCCTGTCAGTTGGGTGCAAACATTTGGTGCTGAAGG
  CTTGGCCTCCTTATTGGACATTCTTAAACGACTTCATGATGAGAAAGAAGAGACTGCTGGGAGTTACGAT
  AGCCGGAACAAGCATGAGATCATTCGCTGCTTGAAAGCTTTTATGAACAACAAGTTTGGAATCAAGACCA
  TGTTGGAGACAGAAGAAGGAATCCTACTGCTGGTCAGAGCCATGGATCCTGCTGTTCCCAACATGATGAT
  TGATGCAGCTAAGCTGCTTTCTGCTCTTTGTATTCTACCGCAGCCAGAGGACATGAATGAAAGGGTTTTG
  GAGGCAATGACAGAAAGAGCTGAGATGGATGAAGTGGAACGTTTCCAGCCGCTGCTGGATGGATTAAAAA
  GTGGAACCACTATTGCACTGAAGGTTGGATGCCTACAGCTGATCAATGCTCTCATCACACCAGCGGAGGA
  ACTTGACTTCCGAGTTCACATCAGAAGTGAACTGATGCGTTTGGGGCTACATCAGGTGTTGCAGGACCTT
  CGAGAGATTGAAAATGAAGATATGAGAGTGCAACTAAATGTGTTTGATGAACAAGGGGAAGAGGATTCCT
  ATGACCTGAAGGGACGGCTGGATGACATTCGCATGGAGATGGATGACTTTAATGAAGTCTTTCAGATTCT
  CTTAAACACAGTGAAGGATTCAAAGGCAGAGCCACACTTCCTTTCCATCCTGCAGCACTTACTCTTGGTC
  CGAAATGACTATGAGGCCAGACCTCAGTACTATAAGTTGATTGAAGAATGTATTTCCCAGATAGTTCTGC
  ACAAGAACGGGGCTGATCCTGACTTCAAGTGCCGGCACCTCCAGATTGAGATTGAGGGATTAATTGATCA
  AATGATTGATAAGACAAAGGTGGAGAAATCTGAAGCCAAAGCTGCAGAGCTGGAAAAGAAGTTGGACTCA
  GAGTTAACAGCCCGACATGAGCTACAGGTGGAAATGAAAAAGATGGAAAGTGACTTTGAGCAGAAGCTTC
  AAGATCTTCAGGGAGAAAAAGATGCACTGCATTCTGAAAAGCAGCAAATTGCCACAGAGAAACAGGACCT
  GGAAGCAGAGGTGTCCCAGCTCACAGGAGAGGTTGCCAAGCTGACAAAGGAACTGGAAGATGCCAAGAAA
  GAAATGGCTTCCCTCTCTGCGGCAGCTATTACTGTACCTCCTTCTGTTCCTAGTCGTGCTCCTGTTCCCC
  CTGCCCCTCCTTTACCTGGTGACTCTGGCACTATTATTCCACCACCACCTGCTCCTGGGGATAGTACCAC
  TCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCACCTCCTTTGCCTGGGGGTGTTTGCATCTCCTCACCC
  CCTTCTTTACCTGGAGGTACTGCTATCTCTCCACCCCCTCCTTTGTCTGGGGATGCTACCATCCCTCCAC
  CCCCTCCTTTGCCTGAGGGTGTTGGCATCCCTTCACCCTCTTCTTTGCCTGGAGGTACTGCCATCCCCCC
  ACCTCCTCCTTTGCCTGGGAGTGCTAGAATCCCCCCACCACCACCTCCTTTGCCTGGGAGTGCTGGAATT
  CCCCCCCCACCTCCTCCCTTGCCTGGAGAAGCAGGAATGCCACCTCCTCCTCCCCCTCTTCCTGGTGGTC
  CTGGAATCCCTCCACCTCCTCCATTTCCCGGAGGCCCTGGCATTCCTCCACCTCCACCCGGAATGGGTAT
  GCCTCCACCTCCCCCATTTGGATTTGGAGTTCCTGCAGCCCCAGTTCTGCCATTTGGATTAACCCCCAAA
  AAGCTTTATAAGCCAGAGGTGCAGCTCCGGAGGCCAAACTGGTCCAAGCTTGTGGCTGAGGACCTCTCCC
  AGGACTGCTTCTGGACAAAGGTGAAGGAGGACCGCTTTGAGAACAATGAACTTTTCGCCAAACTTACCCT
  TACCTTCTCTGCCCAGACCAAGACTTCCAAAGCCAAGAAGGATCAAGAAGGTGGAGAAGAAAAGAAATCT
  GTGCAAAAGAAAAAAGTAAAAGAGTTAAAGGTGTTGGATTCAAAGACAGCCCAGAATCTCTCAATCTTTT
  TGGGTTCCTTCCGCATGCCCTATCAAGAGATTAAGAATGTCATCCTGGAGGTGAATGAGGCTGTTCTGAC
  TGAGTCTATGATCCAGAACCTCATTAAGCAAATGCCAGAGCCAGAGCAGTTAAAAATGCTTTCTGAACTG
  AAGGATGAATATGATGACCTGGCTGAGTCAGAGCAGTTTGGCGTGGTGATGGGCACTGTGCCCCGACTGC
  GGCCTCGCCTCAATGCCATTCTCTTCAAGCTACAATTCAGCGAGCAAGTGGAGAATATCAAGCCAGAGAT
  TGTGTCTGTCACTGCTGCATGTGAGGAGTTACGTAAGAGTGAGAGCTTTTCCAATCTCCTAGAGATTACC
  TTGCTTGTTGGAAATTACATGAATGCTGGCTCCAGAAATGCTGGTGCTTTTGGCTTCAATATCAGCTTCC
  TCTGTAAGCTTCGAGACACCAAGTCCACAGATCAGAAGATGACGTTGTTACACTTCTTGGCTGAGTTGTG
  TGAGAATGACTATCCCGATGTCCTCAAGTTTCCAGACGAGCTTGCCCATGTGGAGAAAGCCAGCCGAGTT
  TCTGCTGAAAACTTGCAAAAGAACCTAGATCAGATGAAGAAACAAATTTCTGATGTGGAACGTGATGTTC
  AGAATTTCCCAGCTGCCACAGATGAAAAAGACAAGTTTGTTGAAAAAATGACCAGCTTTGTGAAGGATGC
  ACAGGAACAGTATAACAAGCTGCGGATGATGCATTCTAACATGGAGACCCTCTATAAGGAGCTGGGCGAG
  TACTTCCTCTTTGACCCCAAGAAGTTGTCTGTTGAAGAATTTTTCATGGATCTTCACAATTTTCGGAATA
  TGTTTTTGCAAGCAGTCAAGGAGAACCAGAAGCGGCGGGAGACAGAAGAAAAGATGAGGCGAGCAAAACT
  AGCCAAGGAGAAGGCAGAGAAGGAGCGGCTAGAGAAGCAGCAGAAGAGAGAGCAACTCATAGACATGAAT
  GCAGAGGGCGATGAGACAGGTGTGATGGACAGTCTTCTAGAAGCCCTGCAGTCAGGGGCAGCATTCCGAC
  GGAAGAGAGGGCCCCGTCAAGCCAACAGGAAGGCCGGGTGTGCAGTCACATCTCTGCTAGCTTCGGAGCT
  GACCAAGGATGATGCCATGGCTGCTGTTCCTGCCAAGGTGTCCAAGAACAGTGAGACATTCCCCACAATC
  CTTGAGGAAGCCAAGGAGTTGGTTGGCCGTGCAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216318 representing NM_005219
  Red=Cloning site Green=Tags(s)
  
  MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRN
  SSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKE
  SSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYD
  SRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVL
  EAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDL
  REIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLV
  RNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDS
  ELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKK
  EMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSP
  PSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGI
  PPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPK
  KLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKS
  VQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSEL
  KDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEIT
  LLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRV
  SAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGE
  YFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMN
  AEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTI
  LEEAKELVGRAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005219
• ORF Size: 3816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005219.3, NM_005219.4, NP_005210.3
• RefSeq Size: 5745 bp
• RefSeq ORF: 3819 bp
• Locus ID: 1729
• Cytogenetics: 5q31.3
• Domains: FH2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton
• MW: 141.2 kDa
• Gene Summary: 'This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'