KCNJ9 (NM_004983) Human Tagged ORF Clone
CAT#: RC216336
KCNJ9 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9)
"NM_004983" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KCNJ9 |
Synonyms | GIRK3; KIR3.3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216336 representing NM_004983
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGGAGAACGCGGCCTTCTCGCCCGGGCAGGAGGAGCCGCCGCGGCGCCGCGGCCGCCAGCGCT ACGTGGAGAAGGATGGCCGGTGCAACGTGCAGCAGGGCAACGTGCGCGAGACATACCGCTACCTGACGGA CCTGTTCACCACGCTGGTGGACCTGCAGTGGCGCCTCAGCCTGTTGTTCTTCGTCCTGGCCTACGCGCTC ACCTGGCTCTTCTTCGGCGCCATCTGGTGGCTGATCGCCTACGGCCGCGGCGACCTGGAGCACCTGGAGG ACACCGCGTGGACGCCGTGCGTCAACAACCTCAACGGCTTCGTGGCCGCCTTCCTCTTCTCCATCGAGAC CGAGACCACCATCGGCTACGGGCACCGCGTCATCACCGACCAGTGCCCCGAGGGCATCGTGCTGCTGCTG CTGCAGGCCATCCTGGGCTCCATGGTGAACGCCTTCATGGTGGGCTGCATGTTCGTCAAGATCTCGCAGC CCAACAAGCGCGCAGCCACGCTCGTCTTCTCCTCGCACGCCGTGGTGTCGCTGCGCGACGGGCGCCTCTG CCTCATGTTCCGCGTGGGCGACTTGCGCTCCTCACACATAGTGGAGGCCTCCATCCGCGCCAAGCTCATC CGCTCGCGCCAGACGCTGGAGGGCGAGTTCATCCCGCTGCACCAGACCGACCTCAGCGTGGGCTTCGACA CGGGAGACGACCGCCTCTTCCTCGTCTCGCCGCTGGTTATCAGCCACGAGATCGACGCCGCCAGCCCCTT CTGGGAGGCGTCGCGCCGTGCCCTCGAGAGGGACGACTTCGAGATCGTCGTTATCCTCGAGGGCATGGTG GAAGCCACGGGAATGACATGCCAAGCTCGGAGCTCCTACCTGGTAGACGAGGTGCTGTGGGGCCACCGCT TCACGTCAGTGCTGACTCTGGAGGACGGCTTCTACGAAGTGGACTATGCCAGCTTTCACGAGACTTTTGA GGTGCCCACACCTTCGTGCAGTGCTCGAGAGCTGGCAGAGGCTGCCGCCCGCCTTGATGCCCATCTCTAC TGGTCCATCCCCAGCCGGCTGGATGAGAAGGTGGAGGAGGAGGGGGCGGGGGAGGGGGCGGGTGGGGAAG CTGGGGCTGACAAGGAGCAGAATGGCTGCCTGCCACCCCCAGAGAGTGAGTCCAAGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216336 representing NM_004983
Red=Cloning site Green=Tags(s) MAQENAAFSPGQEEPPRRRGRQRYVEKDGRCNVQQGNVRETYRYLTDLFTTLVDLQWRLSLLFFVLAYAL TWLFFGAIWWLIAYGRGDLEHLEDTAWTPCVNNLNGFVAAFLFSIETETTIGYGHRVITDQCPEGIVLLL LQAILGSMVNAFMVGCMFVKISQPNKRAATLVFSSHAVVSLRDGRLCLMFRVGDLRSSHIVEASIRAKLI RSRQTLEGEFIPLHQTDLSVGFDTGDDRLFLVSPLVISHEIDAASPFWEASRRALERDDFEIVVILEGMV EATGMTCQARSSYLVDEVLWGHRFTSVLTLEDGFYEVDYASFHETFEVPTPSCSARELAEAAARLDAHLY WSIPSRLDEKVEEEGAGEGAGGEAGADKEQNGCLPPPESESKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004983 |
ORF Size | 1179 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004983.1, NM_004983.2, NP_004974.2 |
RefSeq Size | 3029 bp |
RefSeq ORF | 1182 bp |
Locus ID | 3765 |
Cytogenetics | 1q23.2 |
Domains | IRK |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
MW | 43.8 kDa |
Gene Summary | 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It associates with another G-protein-activated potassium channel to form a heteromultimeric pore-forming complex. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117023 | KCNJ9 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9) |
USD 670.00 |
|
RG216336 | KCNJ9 (GFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9) |
USD 460.00 |
|
RC216336L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9), Myc-DDK-tagged |
USD 620.00 |
|
RC216336L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9), mGFP tagged |
USD 620.00 |
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