ADNP (NM_181442) Human Tagged ORF Clone

CAT#: RC216456

ADNP (Myc-DDK-tagged)-Human activity-dependent neuroprotector homeobox (ADNP), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405758) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216456 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADNP
• Synonyms: ADNP1; HVDAS; MRD28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216456 representing NM_181442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCAACTTCCTGTCAACAATCTTGGCAGTTTAAGAAAAGCCCGGAAAACTGTGAAAAAAATACTTA
  GTGACATTGGGTTGGAATACTGTAAAGAACATATAGAAGATTTTAAACAATTTGAACCTAATGACTTTTA
  TTTGAAAAACACTACATGGGAGGATGTAGGACTGTGGGACCCATCACTTACGAAAAACCAGGACTATCGG
  ACAAAACCTTTCTGCTGCAGCGCTTGTCCATTTTCCTCAAAATTCTTCTCTGCCTACAAAAGTCATTTCC
  GCAATGTCCATAGTGAAGACTTTGAAAATAGGATTCTCCTTAATTGCCCCTACTGTACCTTCAATGCAGA
  CAAAAAGACTTTGGAAACACACATTAAAATATTTCATGCTCCGAACGCCAGCGCACCAAGTAGCAGCCTC
  AGCACTTTCAAAGATAAAAACAAAAATGATGGCCTTAAACCTAAGCAGGCTGACAGTGTAGAGCAAGCTG
  TTTATTACTGTAAGAAGTGCACTTACCGAGATCCTCTTTATGAAATAGTTAGGAAGCACATTTACAGGGA
  ACATTTTCAGCATGTGGCAGCACCTTACATAGCAAAGGCAGGAGAAAAATCACTCAATGGGGCAGTCCCC
  TTAGGCTCGAATGCCCGAGAAGAGAGTAGTATTCACTGCAAGCGATGCCTTTTCATGCCAAAGTCCTATG
  AAGCTTTGGTACAGCATGTCATCGAAGACCATGAACGTATAGGCTATCAGGTCACTGCCATGATTGGGCA
  CACAAATGTAGTGGTTCCCCGATCCAAACCCTTGATGCTAATTGCTCCCAAACCTCAAGACAAGAAGAGC
  ATGGGACTCCCACCAAGGATCGGTTCCCTTGCTTCTGGAAATGTCCGGTCTTTACCATCACAGCAGATGG
  TGAATCGACTCTCAATACCAAAGCCTAACTTAAATTCTACAGGAGTCAACATGATGTCCAGTGTTCATCT
  GCAGCAGAACAACTATGGAGTCAAATCTGTAGGCCAGGGTTACAGTGTTGGTCAGTCAATGAGACTGGGT
  CTAGGTGGCAACGCACCAGTTTCCATTCCTCAACAATCTCAGTCTGTAAAGCAGTTACTTCCAAGTGGAA
  ACGGAAGGTCTTATGGGCTTGGGTCAGAGCAGAGGTCCCAGGCACCAGCAAGATACTCCCTGCAGTCTGC
  TAATGCCTCTTCTCTCTCATCGGGCCAGTTAAAGTCTCCTTCCCTCTCTCAGTCACAGGCATCCAGAGTG
  TTAGGTCAGTCCAGTTCCAAACCTGCTGCAGCTGCCACAGGCCCTCCCCCAGGTAACACTTCCTCAACTC
  AAAAGTGGAAAATATGTACAATCTGTAATGAGCTTTTTCCTGAAAATGTCTATAGTGTGCACTTCGAAAA
  AGAACATAAAGCTGAGAAAGTCCCAGCAGTAGCCAACTACATTATGAAAATACACAATTTTACTAGCAAA
  TGCCTCTACTGTAATCGCTATTTACCCACAGATACTCTGCTCAACCATATGTTAATTCATGGTCTGTCTT
  GTCCATATTGCCGTTCAACTTTCAATGATGTGGAAAAGATGGCCGCACACATGCGGATGGTTCACATTGA
  TGAAGAGATGGGACCTAAAACAGATTCTACTTTGAGTTTTGATTTGACATTGCAGCAGGGTAGTCACACT
  AACATCCATCTCCTGGTAACTACATACAATCTGAGGGATGCCCCAGCTGAATCTGTTGCTTACCATGCCC
  AAAATAATCCTCCAGTTCCTCCAAAGCCACAGCCAAAGGTTCAGGAAAAGGCAGATATCCCTGTAAAAAG
  TTCACCTCAAGCTGCAGTGCCCTATAAAAAAGATGTTGGGAAAACCCTTTGTCCTCTTTGCTTTTCAATC
  CTAAAAGGACCCATATCTGATGCACTTGCACATCACTTACGAGAGAGGCACCAAGTTATTCAGACGGTTC
  ATCCAGTTGAGAAAAAGCTCACCTACAAATGTATCCATTGCCTTGGTGTGTATACCAGCAACATGACCGC
  CTCAACTATCACTCTGCATCTAGTTCACTGCAGGGGCGTTGGAAAGACCCAAAATGGCCAGGATAAGACA
  AATGCACCCTCTCGGCTTAATCAGTCTCCAAGTCTGGCACCTGTGAAGCGCACTTACGAGCAAATGGAAT
  TTCCCTTACTGAAAAAACGAAAGTTAGATGATGATAGTGATTCACCCAGCTTCTTTGAAGAGAAGCCTGA
  AGAGCCTGTTGTTTTAGCTTTAGACCCCAAGGGTCATGAAGATGATTCCTATGAAGCCAGGAAAAGCTTT
  CTAACAAAGTATTTCAACAAACAGCCCTATCCCACCAGGAGAGAAATTGAGAAGCTAGCAGCCAGTTTAT
  GGTTATGGAAGAGTGACATCGCTTCCCATTTTAGTAACAAAAGGAAGAAGTGTGTCCGTGATTGTGAAAA
  GTACAAGCCTGGCGTGTTGCTGGGGTTTAACATGAAAGAATTAAATAAAGTCAAGCATGAGATGGATTTT
  GATGCTGAGTGGCTATTTGAAAATCATGATGAGAAGGATTCCAGAGTCAATGCTAGTAAGACTGCTGACA
  AAAAGCTCAACCTTGGGAAGGAAGATGACAGTTCCTCAGACAGTTTTGAAAATTTGGAAGAAGAATCCAA
  TGAAAGTGGTAGCCCTTTTGACCCTGTTTTTGAAGTTGAACCTAAAATCTCTAACGATAACCCAGAGGAA
  CATGTACTGAAGGTAATTCCTGAGGATGCTTCAGAATCTGAGGAGAAGCTAGACCAAAAAGAGGATGGTT
  CAAAATACGAAACTATTCATTTGACTGAGGAACCAACCAAACTAATGCACAATGCATCTGATAGTGAGGT
  TGACCAAGACGATGTTGTTGAGTGGAAAGACGGTGCTTCTCCATCTGAGAGTGGGCCTGGATCCCAACAA
  GTGTCAGACTTTGAGGACAATACCTGCGAAATGAAACCAGGAACCTGGTCTGACGAGTCTTCCCAAAGCG
  AAGATGCAAGGAGCAGTAAGCCAGCTGCCAAAAAAAAGGCTACCATGCAAGGTGACAGAGAGCAGTTGAA
  ATGGAAGAATAGTTCCTATGGAAAAGTTGAAGGGTTTTGGTCTAAGGACCAGTCACAGTGGAAGAATGCA
  TCTGAGAATGATGAGCGCTTATCTAACCCCCAGATTGAGTGGCAGAATAGCACAATTGACAGTGAGGATG
  GGGAACAGTTTGACAACATGACTGATGGAGTAGCTGAGCCCATGCATGGCAGCTTAGCCGGAGTTAAACT
  GAGCAGCCAACAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216456 representing NM_181442
  Red=Cloning site Green=Tags(s)
  
  MFQLPVNNLGSLRKARKTVKKILSDIGLEYCKEHIEDFKQFEPNDFYLKNTTWEDVGLWDPSLTKNQDYR
  TKPFCCSACPFSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTFNADKKTLETHIKIFHAPNASAPSSSL
  STFKDKNKNDGLKPKQADSVEQAVYYCKKCTYRDPLYEIVRKHIYREHFQHVAAPYIAKAGEKSLNGAVP
  LGSNAREESSIHCKRCLFMPKSYEALVQHVIEDHERIGYQVTAMIGHTNVVVPRSKPLMLIAPKPQDKKS
  MGLPPRIGSLASGNVRSLPSQQMVNRLSIPKPNLNSTGVNMMSSVHLQQNNYGVKSVGQGYSVGQSMRLG
  LGGNAPVSIPQQSQSVKQLLPSGNGRSYGLGSEQRSQAPARYSLQSANASSLSSGQLKSPSLSQSQASRV
  LGQSSSKPAAAATGPPPGNTSSTQKWKICTICNELFPENVYSVHFEKEHKAEKVPAVANYIMKIHNFTSK
  CLYCNRYLPTDTLLNHMLIHGLSCPYCRSTFNDVEKMAAHMRMVHIDEEMGPKTDSTLSFDLTLQQGSHT
  NIHLLVTTYNLRDAPAESVAYHAQNNPPVPPKPQPKVQEKADIPVKSSPQAAVPYKKDVGKTLCPLCFSI
  LKGPISDALAHHLRERHQVIQTVHPVEKKLTYKCIHCLGVYTSNMTASTITLHLVHCRGVGKTQNGQDKT
  NAPSRLNQSPSLAPVKRTYEQMEFPLLKKRKLDDDSDSPSFFEEKPEEPVVLALDPKGHEDDSYEARKSF
  LTKYFNKQPYPTRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDF
  DAEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEE
  HVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQ
  VSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNA
  SENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181442
• ORF Size: 3306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181442.1, NM_181442.2, NM_181442.3, NP_852107.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 3309 bp
• Locus ID: 23394
• Protein Families: Transcription Factors
• MW: 123.4 kDa
• Gene Summary: Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]