ADNP (NM_181442) Human Tagged ORF Clone

CAT#: RC216456L3

Lenti ORF clone of Human activity-dependent neuroprotector homeobox (ADNP), transcript variant 2, Myc-DDK-tagged

Product Images

Double digestion of RC216456L3 using SgfI and MluI

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  Double digestion of RC216456L3 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADNP
• Synonyms: ADNP1; HVDAS; MRD28
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC216456).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_181442
• ORF Size: 3306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181442.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 3309 bp
• Locus ID: 23394
• UniProt ID: Q9H2P0
• Cytogenetics: 20q13.13
• Protein Families: Transcription Factors
• MW: 123.4 kDa
• Gene Summary: Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]