ALLC (NM_018436) Human Tagged ORF Clone

CAT#: RC216471

ALLC (Myc-DDK-tagged)-Human allantoicase (ALLC), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413022) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216471 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ALLC protein (Cat# TP316471). The protein was produced from HEK293T cells transfected with ALLC cDNA clone (Cat# RC216471) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALLC
• Synonyms: ALC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216471 representing NM_018436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGGCATCTGAATCCGTAGGAGGAAAAATTTTATTTGCAACAGATGACTTTTTTGCTCCTGCAG
  AAAACCTCATAAAGAGTGACAGCCCGTGCTTCAAAGAGCATGAATATACGGAGTTTGGGAAATGGATGGA
  TGGCTGGGAGACCAGGAGGAAAAGGATTCCAGGTCACGACTGGTGTGTCCTCAGGCTGGGGATCCAAGGA
  GTCATCCGGGGCTTCGACGTGGACGTTTCTTACTTCACGGGAGATTACGCTCCTCGAGTGTCCATTCAAG
  CAGCAAACTTGGAAGAAGATAAACTACCAGAAATCCCAGAAAGAGGAACCAGGACAGGAGCTGCAGCCAC
  TCCTGAGGAGTTTGAAGCCATTGCTGAGCTAAAATCCGACGACTGGAGTTACTTGGTTCCCATGACTGAG
  CTTAAGCCAGGAAACCCTGCTTCCGGCCACAACTATTTTCTTGTCAATTCCCAGCAGAGATGGACTCATA
  TCAGACTCAACATTTTCCCAGATGGTGGAATTGCACGACTTAGAGTATTCGGTACTGGACAAAAAGACTG
  GACTGCAACTGACCCCAAAGAACCTGCAGACCTAGTGGCCATCGCTTTTGGGGGTGTCTGTGTAGGATTT
  AGTAATGCTAAGTTTGGGCACCCAAACAATATAATAGGAGTTGGCGGGGCAAAGTCTATGGCGGATGGTT
  GGGAAACTGCAAGAAGGCTGGACCGGCCACCAATATTAGAAAATGATGAGAATGGCATTCTCTTGGTTCC
  GGGTTGTGAATGGGCAGTTTTCCGATTGGCACATCCTGGAGTAATAACTCGAATTGAAATTGACACAAAA
  TATTTTGAAGGCAATGCTCCTGACAGCTGTAAAGTGGATGGGTGCATCCTGACAACTCAGGAAGAAGAAG
  CCGTGATCAGGCAAAAGTGGATTCTCCCGGCCCACAAGTGGAAACCACTGCTTCCAGTGACCAAGTTGTC
  TCCCAACCAAAGTCATCTGTTCGATAGCCTGACCCTAGAGCTCCAAGATGTCATCACTCACGCCAGGCTC
  ACCATCGTCCCCGACGGGGGAGTGAGCCGCCTTCGGCTCCGGGGCTTCCCCAGCTCCATCTGCCTCCTGA
  GGCCCCGGGAGAAGCCCATGTTGAAGTTCTCGGTGAGCTTCAAAGCAAACCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216471 representing NM_018436
  Red=Cloning site Green=Tags(s)
  
  MDMASESVGGKILFATDDFFAPAENLIKSDSPCFKEHEYTEFGKWMDGWETRRKRIPGHDWCVLRLGIQG
  VIRGFDVDVSYFTGDYAPRVSIQAANLEEDKLPEIPERGTRTGAAATPEEFEAIAELKSDDWSYLVPMTE
  LKPGNPASGHNYFLVNSQQRWTHIRLNIFPDGGIARLRVFGTGQKDWTATDPKEPADLVAIAFGGVCVGF
  SNAKFGHPNNIIGVGGAKSMADGWETARRLDRPPILENDENGILLVPGCEWAVFRLAHPGVITRIEIDTK
  YFEGNAPDSCKVDGCILTTQEEEAVIRQKWILPAHKWKPLLPVTKLSPNQSHLFDSLTLELQDVITHARL
  TIVPDGGVSRLRLRGFPSSICLLRPREKPMLKFSVSFKANP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018436
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018436.3
• RefSeq Size: 1443
• RefSeq ORF: 1176
• Locus ID: 55821
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 43.4 kDa
• Gene Summary: Allantoicase (EC 3.5.3.4) participates in the uric acid degradation pathway. Its enzymatic activity, like that of urate oxidase (MIM 191540), was lost during vertebrate evolution. [supplied by OMIM, Nov 2008]