WIPI2 (NM_001033520) Human Tagged ORF Clone

CAT#: RC216506

WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY422379) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216506 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WIPI2
• Synonyms: ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216506 representing NM_001033520
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACGCATGTACTTCCCTTTGTGATTTCAGCCGATACGGAAGATGTGTGCATTGTAGAGAGATTGT
  TCTCCAGCAGCCTAGTGGCCATCGTCAGCCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAA
  GGGAACTGAGATCTGCAACTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTG
  ATAGTATGCCTGGAGGAGTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAGGG
  AGACGCCTCCAAACCCTGCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCC
  AGGGAGCGCGACCATCGGAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCG
  GCTCACGACAGTCCTTTAGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGA
  AGGGGACCGTGATTAGGGTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAA
  GAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACT
  GAGACCGTGCACATCTTCAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCG
  GGTACTTCGGGAAAGTGCTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCA
  GGGCAGAGCCTTCGCCACGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATT
  CAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGACCCCCAGG
  AGGGCGGCGAGTGTGCCCTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTT
  GGACTCTGCCTCTCACGACTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGCCTACACA
  GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACA
  GCGAGCACCCGCCCATGATTCTTCGGACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216506 representing NM_001033520
  Red=Cloning site Green=Tags(s)
  
  MFTHVLPFVISADTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRL
  IVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIP
  AHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNT
  ETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATI
  QKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKAYT
  DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033520
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001033520.1, NP_001028692.1
• RefSeq Size: 4295
• RefSeq ORF: 1155
• Locus ID: 26100
• MW: 41.9 kDa
• Gene Summary: WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]). [supplied by OMIM, Mar 2008]