BMP1 (NM_006129) Human Tagged ORF Clone

CAT#: RC216572

BMP1 (Myc-DDK-tagged)-Human bone morphogenetic protein 1 (BMP1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY416856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216572 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP1
• Synonyms: OI13; PCOLC; PCP; PCP2; TLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216572 representing NM_006129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGCGTGGCCCGCCTGCCGCTGCTGCTCGGGCTGCTGCTGCTCCCGCGTCCCGGCCGGCCGCTGG
  ACTTGGCCGACTACACCTATGACCTGGCGGAGGAGGACGACTCGGAGCCCCTCAACTACAAAGACCCCTG
  CAAGGCGGCTGCCTTTCTTGGGGACATTGCCCTGGACGAAGAGGACCTGAGGGCCTTCCAGGTACAGCAG
  GCTGTGGATCTCAGACGGCACACAGCTCGTAAGTCCTCCATCAAAGCTGCAGTTCCAGGAAACACTTCTA
  CCCCCAGCTGCCAGAGCACCAACGGGCAGCCTCAGAGGGGAGCCTGTGGGAGATGGAGAGGTAGATCCCG
  TAGCCGGCGGGCGGCGACGTCCCGACCAGAGCGTGTGTGGCCCGATGGGGTCATCCCCTTTGTCATTGGG
  GGAAACTTCACTGGTAGCCAGAGGGCAGTCTTCCGGCAGGCCATGAGGCACTGGGAGAAGCACACCTGTG
  TCACCTTCCTGGAGCGCACTGACGAGGACAGCTATATTGTGTTCACCTATCGACCTTGCGGGTGCTGCTC
  CTACGTGGGTCGCCGCGGCGGGGGCCCCCAGGCCATCTCCATCGGCAAGAACTGTGACAAGTTCGGCATT
  GTGGTCCACGAGCTGGGCCACGTCGTCGGCTTCTGGCACGAACACACTCGGCCAGACCGGGACCGCCACG
  TTTCCATCGTTCGTGAGAACATCCAGCCAGGGCAGGAGTATAACTTCCTGAAGATGGAGCCTCAGGAGGT
  GGAGTCCCTGGGGGAGACCTATGACTTCGACAGCATCATGCATTACGCTCGGAACACATTCTCCAGGGGC
  ATCTTCCTGGATACCATTGTCCCCAAGTATGAGGTGAACGGGGTGAAACCTCCCATTGGCCAAAGGACAC
  GGCTCAGCAAGGGGGACATTGCCCAAGCCCGCAAGCTTTACAAGTGCCCAGCCTGTGGAGAGACCCTGCA
  AGACAGCACAGGCAACTTCTCCTCCCCTGAATACCCCAATGGCTACTCTGCTCACATGCACTGCGTGTGG
  CGCATCTCTGTCACACCCGGGGAGAAGATCATCCTGAACTTCACGTCCCTGGACCTGTACCGCAGCCGCC
  TGTGCTGGTACGACTATGTGGAGGTCCGAGATGGCTTCTGGAGGAAGGCGCCCCTCCGAGGCCGCTTCTG
  CGGGTCCAAACTCCCTGAGCCTATCGTCTCCACTGACAGCCGCCTCTGGGTTGAATTCCGCAGCAGCAGC
  AATTGGGTTGGAAAGGGCTTCTTTGCAGTCTACGAAGCCATCTGCGGGGGTGATGTGAAAAAGGACTATG
  GCCACATTCAATCGCCCAACTACCCAGACGATTACCGGCCCAGCAAAGTCTGCATCTGGCGGATCCAGGT
  GTCTGAGGGCTTCCACGTGGGCCTCACATTCCAGTCCTTTGAGATTGAGCGCCACGACAGCTGTGCCTAC
  GACTATCTGGAGGTGCGCGACGGGCACAGTGAGAGCAGCACCCTCATCGGGCGCTACTGTGGCTATGAGA
  AGCCTGATGACATCAAGAGCACGTCCAGCCGCCTCTGGCTCAAGTTCGTCTCTGACGGGTCCATTAACAA
  AGCGGGCTTTGCCGTCAACTTTTTCAAAGAGGTGGACGAGTGCTCTCGGCCCAACCGCGGGGGCTGTGAG
  CAGCGGTGCCTCAACACCCTGGGCAGCTACAAGTGCAGCTGTGACCCCGGGTACGAGCTGGCCCCAGACA
  AGCGCCGCTGTGAGGCTGCTTGTGGCGGATTCCTCACCAAGCTCAACGGCTCCATCACCAGCCCGGGCTG
  GCCCAAGGAGTACCCCCCCAACAAGAACTGCATCTGGCAGCTGGTGGCCCCCACCCAGTACCGCATCTCC
  CTGCAGTTTGACTTATTTGAGACAGAGGGCAATGATGTGTGCAAGTACGACTTCGTGGAGGTGCGCAGTG
  GACTCACAGCTGACTCCAAGCTGCATGGCAAGTTCTGTGGTTCTGAGAAGCCCGAGGTCATCACCTCCCA
  GTACAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTGTCCAAAAAGGGCTTCAAGGCCCACTTC
  TTCTCAGACAAGGACGAGTGCTCCAAGGATAACGGCGGCTGCCAGCAGGACTGCGTCAACACGTTCGGCA
  GTTATGAGTGCCAATGCCGCAGTGGCTTCGTCCTCCATGACAACAAGCACGACTGCAAAGAAGCCGGCTG
  TGACCACAAGGTGACATCCACCAGTGGTACCATCACCAGCCCCAACTGGCCTGACAAGTATCCCAGCAAG
  AAGGAGTGCACGTGGGCCATCTCCAGCACCCCCGGGCACCGGGTCAAGCTGACCTTCATGGAGATGGACA
  TCGAGTCCCAGCCTGAGTGTGCCTACGACCACCTAGAGGTGTTCGACGGGCGAGACGCCAAGGCCCCCGT
  CCTCGGCCGCTTCTGTGGGAGCAAGAAGCCCGAGCCCGTCCTGGCCACAGGCAGCCGCATGTTCCTGCGC
  TTCTACTCAGATAACTCGGTCCAGCGAAAGGGCTTCCAGGCCTCCCACGCCACAGAGTGCGGGGGCCAGG
  TACGGGCAGACGTGAAGACCAAGGACCTTTACTCCCACGCCCAGTTTGGCGACAACAACTACCCTGGGGG
  TGTGGACTGTGAGTGGGTCATTGTGGCCGAGGAAGGCTACGGCGTGGAGCTCGTGTTCCAGACCTTTGAG
  GTGGAGGAGGAGACCGACTGCGGCTATGACTACATGGAGCTCTTCGACGGCTACGACAGCACAGCCCCCA
  GGCTGGGGCGCTACTGTGGCTCAGGGCCTCCTGAGGAGGTGTACTCGGCGGGAGATTCTGTCCTGGTGAA
  GTTCCACTCGGATGACACCATCACCAAAAAAGGTTTCCACCTGCGATACACCAGCACCAAGTTCCAGGAC
  ACACTCCACAGCAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216572 representing NM_006129
  Red=Cloning site Green=Tags(s)
  
  MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQ
  AVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIG
  GNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGI
  VVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRG
  IFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVW
  RISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSS
  NWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAY
  DYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCE
  QRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRIS
  LQFDLFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHF
  FSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSK
  KECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDAKAPVLGRFCGSKKPEPVLATGSRMFLR
  FYSDNSVQRKGFQASHATECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFE
  VEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQD
  TLHSRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006129
• ORF Size: 2958 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006129.1, NM_006129.2, NM_006129.3, NM_006129.4, NP_006120.1
• RefSeq Size: 3552 bp
• RefSeq ORF: 2961 bp
• Locus ID: 649
• Cytogenetics: 8p21.3
• Protein Families: Druggable Genome, Protease
• MW: 111.21 kDa
• Gene Summary: 'This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]'