MNK2 (MKNK2) (NM_199054) Human Tagged ORF Clone

CAT#: RC216704

MKNK2 (Myc-DDK-tagged)-Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404728) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216704 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MKNK2 protein (Cat# TP316704). The protein was produced from HEK293T cells transfected with MKNK2 cDNA clone (Cat# RC216704) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MKNK2
• Synonyms: GPRK7; MNK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216704 representing NM_199054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGAAACCAGCCGAACTTCAGGGTTTCCACCGTTCGTTCAAGGGGCAGAACCCCTTCGAGC
  TGGCCTTCTCCCTAGACCAGCCCGACCACGGAGACTCTGACTTTGGCCTGCAGTGCTCAGCCCGCCCTGA
  CATGCCCGCCAGCCAGCCCATTGACATCCCGGACGCCAAGAAGAGGGGCAAGAAGAAGAAGCGCGGCCGG
  GCCACCGACAGCTTCTCGGGCAGGTTTGAAGACGTCTACCAGCTGCAGGAAGATGTGCTGGGGGAGGGCG
  CTCATGCCCGAGTGCAGACCTGCATCAACCTGATCACCAGCCAGGAGTACGCCGTCAAGATCATTGAGAA
  GCAGCCAGGCCACATTCGGAGCAGGGTTTTCAGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACACAGG
  AACGTCCTAGAGCTGATTGAGTTCTTCGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAGAAGATGCGGG
  GAGGCTCCATCCTGAGCCACATCCACAAGCGCCGGCACTTCAACGAGCTGGAGGCCAGCGTGGTGGTGCA
  GGACGTGGCCAGCGCCTTGGACTTTCTGCATAACAAAGGCATCGCCCACAGGGACCTAAAGCCGGAAAAC
  ATCCTCTGTGAGCACCCCAACCAGGTCTCCCCCGTGAAGATCTGTGACTTCGACCTGGGCAGCGGCATCA
  AACTCAACGGGGACTGCTCCCCTATCTCCACCCCGGAGCTGCTCACTCCGTGCGGCTCGGCGGAGTACAT
  GGCCCCGGAGGTAGTGGAGGCCTTCAGCGAGGAGGCTAGCATCTACGACAAGCGCTGCGACCTGTGGAGC
  CTGGGCGTCATCTTGTATATCCTACTCAGCGGCTACCCGCCCTTCGTGGGCCGCTGTGGCAGCGACTGCG
  GCTGGGACCGCGGCGAGGCCTGCCCTGCCTGCCAGAACATGCTGTTTGAGAGCATCCAGGAGGGCAAGTA
  CGAGTTCCCCGACAAGGACTGGGCCCACATCTCCTGCGCTGCCAAAGACCTCATCTCCAAGCTGCTGGTC
  CGTGACGCCAAGCAGAGGCTGAGTGCCGCCCAAGTCCTGCAGCACCCCTGGGTTCAGGGGTGCGCCCCGG
  AGAACACCTTGCCCACTCCCATGGTCCTGCAGAGGAACAGCTGTGCCAAAGACCTCACGTCCTTCGCGGC
  TGAGGCCATTGCCATGAACCGGCAGCTGGCCCAGCACGACGAGGACCTGGCTGAGGAGGAGGCCGCGGGG
  CAGGGCCAGCCCGTCCTGGTCCGAGCTACCTCACGCTGCCTGCAGCTGTCTCCACCCTCCCAGTCCAAGC
  TGGCGCAGCGGCGGCAAAGGGCCAGTCTGTCCTCGGCCCCAGTGGTCCTGGTGGGAGACCACGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216704 representing NM_199054
  Red=Cloning site Green=Tags(s)
  
  MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIPDAKKRGKKKKRGR
  ATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHR
  NVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPEN
  ILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWS
  LGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLV
  RDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDLTSFAAEAIAMNRQLAQHDEDLAEEEAAG
  QGQPVLVRATSRCLQLSPPSQSKLAQRRQRASLSSAPVVLVGDHA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199054
• ORF Size: 1395 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199054.1, NM_199054.2, NP_951009.1
• RefSeq Size: 3795
• RefSeq ORF: 1398
• Locus ID: 2872
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Insulin signaling pathway, MAPK signaling pathway
• MW: 51.7 kDa
• Gene Summary: This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]