MNK2 (MKNK2) (NM_199054) Human Untagged Clone

CAT#: SC307857

MKNK2 (untagged)-Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MKNK2
• Synonyms: GPRK7; MNK2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_199054 edited
  ATGGTGCAGAAGAAACCAGCCGAACTTCAGGGTTTCCACCGTTCGTTCAAGGGGCAGAAC
  CCCTTCGAGCTGGCCTTCTCCCTAGACCAGCCCGACCACGGAGACTCTGACTTTGGCCTG
  CAGTGCTCAGCCCGCCCTGACATGCCCGCCAGCCAGCCCATTGACATCCCGGACGCCAAG
  AAGAGGGGCAAGAAGAAGAAGCGCGGCCGGGCCACCGACAGCTTCTCGGGCAGGTTTGAA
  GACGTCTACCAGCTGCAGGAAGATGTGCTGGGGGAGGGCGCTCATGCCCGAGTGCAGACC
  TGCATCAACCTGATCACCAGCCAGGAGTACGCCGTCAAGATCATTGAGAAGCAGCCAGGC
  CACATTCGGAGCAGGGTTTTCAGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACACAGG
  AACGTCCTAGAGCTGATTGAGTTCTTCGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAG
  AAGATGCGGGGAGGCTCCATCCTGAGCCACATCCACAAGCGCCGGCACTTCAACGAGCTG
  GAGGCCAGCGTGGTGGTGCAGGACGTGGCCAGCGCCTTGGACTTTCTGCATAACAAAGGC
  ATCGCCCACAGGGACCTAAAGCCGGAAAACATCCTCTGTGAGCACCCCAACCAGGTCTCC
  CCCGTGAAGATCTGTGACTTCGACCTGGGCAGCGGCATCAAACTCAACGGGGACTGCTCC
  CCTATCTCCACCCCGGAGCTGCTCACTCCGTGCGGCTCGGCGGAGTACATGGCCCCGGAG
  GTAGTGGAGGCCTTCAGCGAGGAGGCTAGCATCTACGACAAGCGCTGCGACCTGTGGAGC
  CTGGGCGTCATCTTGTATATCCTACTCAGCGGCTACCCGCCCTTCGTGGGCCGCTGTGGC
  AGCGACTGCGGCTGGGACCGCGGCGAGGCCTGCCCTGCCTGCCAGAACATGCTGTTTGAG
  AGCATCCAGGAGGGCAAGTACGAGTTCCCCGACAAGGACTGGGCCCACATCTCCTGCGCT
  GCCAAAGACCTCATCTCCAAGCTGCTGGTCCGTGACGCCAAGCAGAGGCTGAGTGCCGCC
  CAAGTCCTGCAGCACCCCTGGGTTCAGGGGTGCGCCCCGGAGAACACCTTGCCCACTCCC
  ATGGTCCTGCAGAGGAACAGCTGTGCCAAAGACCTCACGTCCTTCGCGGCTGAGGCCATT
  GCCATGAACCGGCAGCTGGCCCAGCACGACGAGGACCTGGCTGAGGAGGAGGCCGCGGGG
  CAGGGCCAGCCCGTCCTGGTCCGAGCTACCTCACGCTGCCTGCAGCTGTCTCCACCCTCC
  CAGTCCAAGCTGGCGCAGCGGCGGCAAAGGGCCAGTCTGTCCTCGGCCCCAGTGGTCCTG
  GTGGGAGACCACGCCTGACCCTCCCATCTCCCCTCTGTACATAGGTCACCCGTCCCCCAA
  TCAAATCTAAAGGTTTTTTAAGCTATCGCCAGCCGGTGTCCAGCGGGCTGCCCCTCCTCT
  GCCTGGATTCCCAGGCACTAAGCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199054 unedited
  TATTACCCCGCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGCCCTCCCCCGCTGGCGGGGCCCGGACAGAAGATGGTGCAGAAGAA
  ACCAGCCGAACTTCAGGGTTTCCACCGTTCGTTCAAGGGGCAGAACCCCTTCGAGCTGGC
  CTTCTCCCTAGACCAGCCCGACCACGGAGACTCTGACTTTGGCCTGCAGTGCTCAGCCCG
  CCCTGACATGCCCGCCAGCCAGCCCATTGACATCCCGGACGCCAAGAAGAGGGGCAAGAA
  GAAGAAGCGCGGCCGGGCCACCGACAGCTTCTCGGGCAGGTTTGAAGACGTCTACCAGCT
  GCAGGAAGATGTGCTGGGGGAGGGCGCTCATGCCCGAGTGCAGACCTGCATCAACCTGAT
  CACCAGCCAGGAGTACGCCGTCAAGATCATTGAGAAGCAGCCAGGCCACATTCGGAGCAG
  GGTTTTCAGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACACAGGAACGTCCTAGAGCT
  GATTGAGTTCTTCGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAGAAGAGCGGNGAGGC
  TCCATCCTGAGCCACATCCACAAGCGCCGGCACTTCAACGAGCTGGNAGCCANCGTGGGT
  GGTGCAAGNACAGTGGCCAACGCCCTTGGACTTTCTGCATAACAAAGGCATCGCCCACAG
  GGACCTAAAGCCCGGAAACATCCTCTGTGAGCACCCCAACCAGGTCTCCCCGCGAAGGAC
  TTGTGACTTCGACCTGGGCAGCGGCATCAACTCAACGGGGGACTGGTTCCCTTACTCAAC
  CCCGGAGCTGCTACTTCCGGCGGTTCGGGGGAGTAATGCCCCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_199054 unedited
  TTACTTGNACCGCGGCCGCATTCTANNGATCGGTTTTTTTTTTTTTTTTTTGGCTTTTAA
  AAACATCGTAACATTAACACATGGCCGTTCACCGTCCCCCAGCGATGGGAGCTGGCCTGG
  GGCCCAGGGTCCTCCAGGATCTTCACTCATTCACAGTAACGGTTCTGACCAGTCCTCCAG
  GTCGCACGTGGATGCGACAGGGGTGGGGAGGGAGGAGGAAGTGACTGTCCCACCTTCAGA
  AAAAAAAAAACAAACAAACAAACAAACGCTGCTAGCCACTCAGCTTTAGAGACCCGATGG
  CTATGGGCGCCTGCAGCGGGCGGGGGTCCATTTGCTTGTTCTTTGATACAAAAAGGCAGA
  GAATCCCCCGTTACGAAACATGGAATCACTGACAGGCGAGAAGTGATGGGGGAAAGGGGT
  GGNGCGGAATGCCCCACCCCCCCCAGGGGTCTTTGGAAGGGGCAGTCCACAGATATGGGC
  AGTGGGGACCAGGGAAGGCAGAGCACCCCCACGGCCACCGGACTGTGACCATCATACGAG
  ATTCAGGAGGGGCAGCANGGCCAGGCAGACGGTCTCCGAGGCCCCCACCCCCAGGCCCCC
  CGACATTCAAGTATTCTTCAAGAACAGGGAAGCAAAGTCCATCGATGTGTTGTTTTTTTT
  TAAAGAAAAACTAAAAAACTAAACAGGAGGAAAGAATCCCGACCCGCGGATTTAGAAACC
  AGAAGGGCTTGCCCCACCTGGTGGAGCTCGGGGGCTGCCCCCCGGAAGGCGGGCGGTGAC
  CCTAACCGCGCGCACTTTCTAAAGTGGAAACCCTGGATTGCATAGAACGTTCCCACCCGC
  CGGGGAGGGGGTAGACAGCTCCCCAACCCCCCGGGGGTTGGAACATGGAGGTGGGGGGGA
  ATTGACAAACCCCCCAATTCTGGGGTCCCCGAAGGGAGCCCAGGCCCAACCCCTTTTCCT
  GAAGTGAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199054
• ORF Size: 1398 bp
• Insert Size: 3400
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.

Reference Data

• RefSeq: NM_199054.1, NP_951009.1
• RefSeq Size: 3795
• RefSeq ORF: 1398
• Locus ID: 2872
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Insulin signaling pathway, MAPK signaling pathway
• Gene Summary: This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) is the longer transcript and it encodes the longer protein (isoform 2, also known as isoform a), which is located in the cytoplasm and has an MAP kinase-binding region at the C-terminus.