GJA3 (NM_021954) Human Tagged ORF Clone

CAT#: RC216756

GJA3 (Myc-DDK-tagged)-Human gap junction protein, alpha 3, 46kDa (GJA3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GJA3
• Synonyms: CTRCT14; CX46; CZP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216756 representing NM_021954
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGCTTTCTGGGAAGACTCTTAGAAAATGCACAGGAGCACTCCACGGTCATCGGCAAGG
  TTTGGCTGACCGTGCTGTTCATCTTCCGCATCTTGGTGCTGGGGGCCGCGGCGGAGGACGTGTGGGGCGA
  TGAGCAGTCAGACTTCACCTGCAACACCCAGCAGCCGGGCTGCGAGAACGTCTGCTACGACAGGGCCTTC
  CCCATCTCCCACATCCGCTTCTGGGCGCTGCAGATCATCTTCGTGTCCACGCCCACCCTCATCTACCTGG
  GCCACGTGCTGCACATCGTGCGCATGGAAGAGAAGAAGAAAGAGAGGGAGGAGGAGGAGCAGCTGAAGAG
  AGAGAGCCCCAGCCCCAAGGAGCCACCGCAGGACAATCCCTCGTCGCGGGACGACCGCGGCAGGGTGCGC
  ATGGCCGGGGCGCTGCTGCGGACCTACGTCTTCAACATCATCTTCAAGACGCTGTTCGAGGTGGGCTTCA
  TCGCCGGCCAGTACTTTCTGTACGGCTTCGAGCTGAAGCCGCTCTACCGCTGCGACCGCTGGCCCTGCCC
  CAACACGGTGGACTGCTTCATCTCCAGGCCCACGGAGAAGACCATCTTCATCATCTTCATGCTGGCGGTG
  GCCTGCGCGTCCCTGCTGCTCAACATGCTGGAGATCTACCACCTGGGCTGGAAGAAGCTCAAGCAGGGCG
  TGACCAGCCGCCTCGGCCCGGACGCCTCCGAGGCCCCGCTGGGGACAGCCGATCCCCCGCCCCTGCCCCC
  CAGCTCCCGGCCGCCCGCCGTTGCCATCGGGTTCCCACCCTACTATGCGCACACCGCTGCGCCCCTGGGA
  CAGGCCCGCGCCGTGGGCTACCCCGGGGCCCCGCCACCAGCCGCGGACTTCAAAATGCTAGCCCTGACCG
  AGGCGCGCGGAAAGGGCCAGTCCGCCAAGCTCTACAACGGCCACCACCACCTGCTGATGACTGAGCAGAA
  CTGGGCCAACCAGGCGGCCGAGCGGCAGCCCCCGGCGCTCAAGGCTTACCCGGCAGCGTCCACGCCTGCA
  GCCCCCAGCCCCGTCGGCAGCAGCTCCCCGCCACTCGCGCACGAGGCTGAGGCGGGCGCGGCGCCCCTGC
  TGCTGGATGGGAGCGGCAGCAGTCTGGAGGGGAGCGCCCTGGCAGGGACCCCCGAGGAGGAGGAGCAGGC
  CGTGACCACCGCGGCCCAGATGCACCAGCCGCCCTTGCCCCTCGGAGACCCAGGTCGGGCCAGCAAGGCC
  AGCAGGGCCAGCAGCGGGCGGGCCAGACCGGAGGACTTGGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216756 representing NM_021954
  Red=Cloning site Green=Tags(s)
  
  MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEDVWGDEQSDFTCNTQQPGCENVCYDRAF
  PISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEEEQLKRESPSPKEPPQDNPSSRDDRGRVR
  MAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFELKPLYRCDRWPCPNTVDCFISRPTEKTIFIIFMLAV
  ACASLLLNMLEIYHLGWKKLKQGVTSRLGPDASEAPLGTADPPPLPPSSRPPAVAIGFPPYYAHTAAPLG
  QARAVGYPGAPPPAADFKMLALTEARGKGQSAKLYNGHHHLLMTEQNWANQAAERQPPALKAYPAASTPA
  APSPVGSSSPPLAHEAEAGAAPLLLDGSGSSLEGSALAGTPEEEEQAVTTAAQMHQPPLPLGDPGRASKA
  SRASSGRARPEDLAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021954
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021954.1, NM_021954.2, NM_021954.3, NP_068773.2
• RefSeq Size: 1308 bp
• RefSeq ORF: 1308 bp
• Locus ID: 2700
• Cytogenetics: 13q12.11
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 47.2 kDa
• Gene Summary: 'The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3). [provided by RefSeq, Jan 2010]'