PDE5A (NM_033437) Human Tagged ORF Clone

CAT#: RC216759

PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE5A
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216759 representing NM_033437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACA
  CCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGCCCCTGGAACACCAACCAG
  GAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTG
  AGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAG
  GGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATG
  TCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAA
  GACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAG
  TTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCC
  CTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAG
  ACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGTGTAGCCCAGGCCATCA
  ACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTGCTGCTTATTTGGCATT
  TTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTG
  CTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTG
  CCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTC
  TTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGGGAACAT
  GATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAATACTATGGAACCACTTAATATCCCAG
  ATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAAACCAGCAGTGCATTAG
  AAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAAT
  AAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTG
  TCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCA
  AATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTACAGTCG
  TTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGCTTCAGTGACTTTGAGC
  TGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACCTTGTGCAGAACTTCCA
  GATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTAT
  CATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGTTTGCTGCTCTAAAAGCAGGCAAAATTCAGA
  ACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGG
  TGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATCATGGAA
  CACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATTCTCAGTGGCCTCTCCA
  TTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAGACCTAGCACTGTACAT
  TAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTCAATTTGGAAGATCCTCATCAAAAG
  GAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAAC
  AACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACAT
  AGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTCATAGAT
  GCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTCCCTTTGCTAGATGGCT
  GCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGCTGATTAATGGGGAAAG
  CGGCCAGGCCAAGCGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216759 representing NM_033437
  Red=Cloning site Green=Tags(s)
  
  MVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTV
  SFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCE
  DSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYK
  TQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQV
  LLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREH
  DANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVN
  KMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQS
  LAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAY
  HNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIME
  HHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQK
  ELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFID
  AICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033437
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033437.1, NM_033437.2, NM_033437.3, NP_246273.2
• RefSeq Size: 6788
• RefSeq ORF: 2472
• Locus ID: 8654
• Domains: PDEase, GAF
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 93.6 kDa
• Gene Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]