LIPT1 (NM_145199) Human Tagged ORF Clone

CAT#: RC216793L4

Lenti-ORF clone of LIPT1 (mGFP-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: LIPT1
• Synonyms: LIPT1D
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC216793).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_145199
• ORF Size: 1119 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145199.1, NP_660200.1
• RefSeq Size: 1288
• RefSeq ORF: 1122
• Locus ID: 51601
• Domains: BPL_LipA_LipB
• Protein Pathways: Lipoic acid metabolism, Metabolic pathways
• MW: 42.3 kDa
• Gene Summary: The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, the protein encoded by this gene transfers the lipoyl moiety to apoproteins. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 13. Read-through transcription also exists between this gene and the neighboring downstream mitochondrial ribosomal protein L30 (MRPL30) gene. [provided by RefSeq, Mar 2011]