A1CF (NM_138932) Human Tagged ORF Clone

CAT#: RC216861

A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY408476) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216911 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified A1CF protein (Cat# TP316861). The protein was produced from HEK293T cells transfected with A1CF cDNA clone (Cat# RC216861) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A1CF
• Synonyms: ACF; ACF64; ACF65; APOBEC1CF; ASP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCAAATCACAAATCCGGGGATGGATTGAGCGGCACTCAGAAGGAAGCAGCCCTCCGCGCACTGG
  TCCAGCGCACAGGATATAGCTTGGTCCAGGAAAATGGACAAAGAAAATATGGTGGCCCTCCACCTGGTTG
  GGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAACTTCCCCGAGACCTTTTTGAGGAT
  GAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGAGAATGATGATGGATTTTAATGGCA
  ACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGCCAAGAATGCAATCAAGCAACTTAA
  TAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGTGTGGACAACTGCCGATTATTTGTT
  GGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGATGAAAAAGGTTACTGAAGGTGTTG
  TCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCGAGGCTTTGCCTTCGTGGAGTATGA
  GAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGAAGAATTCAGTTATGGGGACATGGT
  ATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATACAATGTCTTCAGTGAAAATCCTAT
  ATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAAGGAATTCAACAATATCAAACCAGG
  TGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCACTTCAGTAACCGAGAAGATGCAGTT
  GAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCATTGAAGTCACCCTAGCAAAACCAG
  TGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAGGGGCACCATGCTGCAAGGAGAGTA
  TACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTTGGAGCTCCTGTCTTCTATGCCCCC
  CAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAGGACATCTCAGCAACAGAGCCATTA
  TCCGAGCCCCTTCTGTTAGAGAAATTTACATGAATGTACCTGTAGGGGCTGCGGGAGTGAGAGGACTGGG
  CGGCCGTGGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCAGGTCAAAGGAGACAAAAGAGAAGAC
  AAACTCTATGACATTTTACCTGGGATGGAGCTCACCCCAATGAATCCTGTCACATTAAAACCCCAAGGAA
  TTAAACTCGCTCCCCAGATATTAGAAGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCT
  GCACTCTGCTATTGGACAAGACCAAAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGC
  CAGAATCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGACGTATG
  CAGCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGGCGATGGCACCATGGCTACTGCTGC
  TGCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAACTGCACCCGTGTCTGCAGCCCAGCTC
  AAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCAACTTTTGCAG
  TGACTGCCCGAGGGGATGGATATGGCACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFED
  ELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFV
  GGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHG
  IAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNREDAV
  EAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYLGAPVFYAP
  QTYAAIPSLHFPATKGHLSNRAIIRAPSVREIYMNVPVGAAGVRGLGGRGYLAYTGLGRGYQVKGDKRED
  KLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALAS
  QNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQL
  KQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138932
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138932.1, NM_138932.2, NP_620310.1
• RefSeq Size: 9293 bp
• RefSeq ORF: 1785 bp
• Locus ID: 29974
• MW: 65.2 kDa
• Gene Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]