PIGW (NM_178517) Human Tagged ORF Clone

CAT#: RC217028

PIGW (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIGW
• Synonyms: Gwt1; HPMRS5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217028 ORF sequence, codon optimized.
  Due to the complexity of NM_178517, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAAAAGCAGATGAAGGAAGCTTTTGTCAGTAACCTCAATGGAACCACCGTGCTGGAAATCACCC
  AGGGATTGTGCTTTCCTGCATTCTGTATCCTGTGCAGAGGGTTCCTGATCATTTTCTCACAGTACTTGTG
  TTCTTTTTCACCTACCTGGAAAACTAGATTCCTCACTGACTTTGTTGTCCTAATAGTTCCCATGGTAGCC
  ACTTTGACCATTTGGGCTTCATTTATCCTCCTTGAGCTTCTCGGTGTAATTATCTTTGGGGCAGGGCTGT
  TGTATCAAATATACCGAAGGAGGACCTGCTATGCCAGACTGCCTTTCCTAAAAATCCTTGAAAAATTCTT
  GAACATCAGTCTAGAATCAGAATACAATCCAGCCATCTCCTGTTTCCGTGTAATTACCAGTGCGTTTACT
  GCTATTGCTATTTTGGCTGTGGACTTCCCACTTTTTCCCAGAAGATTTGCCAAAACTGAGCTCTATGGGA
  CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTGTTTTTGGGTCTGCAATGGTTTGTCTAGAGGTCAGGAG
  GAGAAAATATATGGAAGGGTCCAAATTGCATTACTTTACAAACTCATTGTACTCTGTTTGGCCATTAGTC
  TTCCTAGGAATCGGACGATTAGCCATTATAAAATCAATAGGCTATCAGGAACATTTAACAGAGTATGGAG
  TTCACTGGAACTTTTTCTTTACCATAATAGTTGTGAAATTGATAACACCACTGCTGTTGATTATTTTTCC
  CCTAAATAAGTCCTGGATTATTGCCCTCGGCATTACTGTATTATACCAGCTAGCCCTTGACTTTACCTCA
  CTGAAGAGGTTAATATTATATGGCACTGATGGTAGTGGCACACGGGTTGGTCTATTAAATGCCAACCGCG
  AAGGAATAATCTCTACCCTGGGGTATGTGGCAATACACATGGCTGGTGTGCAAACAGGGTTATATATGCA
  TAAGAACCGATCACATATCAAAGACTTGATAAAAGTAGCCTGTTTTCTTTTACTGGCAGCTATTAGCCTC
  TTCATATCTCTTTACGTAGTTCAAGTAAATGTAGAAGCAGTATCTCGAAGAATGGCAAATTTAGCCTTTT
  GTATTTGGATAGTTGCTTCTAGCCTGATCCTTCTTAGTAGTTTATTACTGGGTGATATAATTTTGAGTTT
  TGCCAAATTTCTAATTAAAGGAGCTCTAGTACCATGTTCTTGGAAACTTATCCAGTCACCTGTTACAAAT
  AAAAAGCATTCAGAATCTCTAGTCCCTGAAGCCGAAAGAATGGAACCCAGTCTTTGTTTAATCACAGCTC
  TAAACAGAAAACAGTTAATATTTTTCTTGCTGTCAAATATAACAACTGGCCTGATCAACCTGATGGTAGA
  TACATTACACAGCAGTACCTTGTGGGCCTTATTTGTGGTCAATCTCTATATGTTTTCCAACTGTTTAATT
  GTATATGTACTATATTTGCAAGATAAGACTGTACAATTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217028 representing NM_178517
  Red=Cloning site Green=Tags(s)
  
  MSEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVA
  TLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPAISCFRVITSAFT
  AIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLV
  FLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTS
  LKRLILYGTDGSGTRVGLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISL
  FISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTN
  KKHSESLVPEAERMEPSLCLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLYMFSNCLI
  VYVLYLQDKTVQFW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178517
• ORF Size: 1512 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178517.1, NM_178517.2, NM_178517.3, NM_178517.4, NP_848612.1
• RefSeq Size: 3050
• RefSeq ORF: 1515
• Locus ID: 284098
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 56.9 kDa
• Gene Summary: The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017]