WAVE 1 (WASF1) (NM_001024936) Human Tagged ORF Clone
CAT#: RC217079L4
- LentiORF®
Lenti-ORF clone of WASF1 (mGFP-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 4
"NM_001024936" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | WASF1 |
Synonyms | NEDALVS; SCAR1; WAVE; WAVE1 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC217079).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001024936 |
ORF Size | 1677 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024936.1, NP_001020107.1 |
RefSeq Size | 2987 bp |
RefSeq ORF | 1680 bp |
Locus ID | 8936 |
Cytogenetics | 6q21 |
Protein Families | Druggable Genome |
Protein Pathways | Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
MW | 61.7 kDa |
Gene Summary | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302315 | WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 4 |
USD 760.00 |
|
RC217079 | WASF1 (Myc-DDK-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 4 |
USD 420.00 |
|
RG217079 | WASF1 (GFP-tagged) - Human WAS protein family, member 1 (WASF1), transcript variant 4 |
USD 460.00 |
|
RC217079L3 | Lenti-ORF clone of WASF1 (Myc-DDK-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 4 |
USD 620.00 |
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