WAVE 1 (WASF1) (NM_001024936) Human Untagged Clone

CAT#: SC302315

WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WASF1
• Synonyms: NEDALVS; SCAR1; WAVE; WAVE1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001024936, the custom clone sequence may differ by one or more nucleotides
  ATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCACACAGCACTGCCTAGA
  GGCATTAAGAATGAACTGGAATGTGTAACCAATATTTCCTTGGCAAATATAATTAGACAA
  CTAAGTAGCCTAAGTAAATATGCTGAAGATATATTTGGAGAATTATTCAATGAAGCACAT
  AGTTTTTCCTTCAGAGTCAACTCATTGCAAGAACGTGTGGACCGTTTATCTGTTAGTGTT
  ACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAGATATAACAATGAGGAAAGCT
  TTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAGACTTTGCCTATTCCA
  TTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAATATACTCACTCCTTAT
  AGAGATGATGGTAAAGAAGGTCTGAAGTTTTATACCAATCCTTCGTATTTCTTTGATCTA
  TGGAAAGAAAAAATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGGAAGCAGAAG
  CAGAAAAATCTAGATCGTCCTCATGAACCAGAAAAAGTGCCAAGAGCACCTCATGACAGG
  CGGCGAGAATGGCAGAAGCTGGCCCAAGGTCCAGAGCTGGCTGAAGATGATGCTAATCTC
  TTACATAAGCATATTGAAGTTGCTAATGGCCCAGCCTCTCATTTTGAAACAAGACCTCAG
  ACATACGTGGATCATATGGATGGATCTTACTCACTTTCTGCCTTGCCATTTAGTCAGATG
  AGTGAGCTTCTGACTAGAGCTGAGGAAAGGGTATTAGTCAGACCACATGAACCACCTCCA
  CCTCCACCAATGCATGGAGCAGGAGATGCAAAACCGATACCCACCTGTATCAGTTCTGCT
  ACAGGTTTGATAGAAAATCGCCCTCAGTCACCAGCTACAGGCAGAACACCTGTGTTTGTG
  AGCCCCACTCCCCCACCTCCTCCACCACCTCTTCCATCTGCCTTGTCAACTTCCTCATTA
  AGAGCTTCAATGACTTCAACTCCTCCCCCTCCAGTACCTCCCCCACCTCCACCTCCAGCC
  ACTGCTTTGCAAGCTCCAGCAGTACCACCACCTCCAGCTCCTCTTCAGATTGCCCCTGGA
  GTTCTTCACCCAGCTCCTCCTCCAATTGCACCTCCTCTAGTACAGCCCTCTCCACCAGTA
  GCTAGAGCTGCCCCAGTATGTGAGACTGTACCAGTTCATCCACTCCCACAAGGTGAAGTT
  CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTGCCTCCACCTGGCATTCGACCATCA
  TCACCTGTCACAGTTACAGCTCTTGCTCATCCTCCCTCTGGGCTACATCCAACTCCATCT
  ACTGCCCCAGGTCCCCATGTTCCATTAATGCCTCCATCTCCTCCATCACAAGTTATACCT
  GCTTCTGAGCCAAAGCGCCATCCATCAACCCTACCTGTAATCAGTGATGCCAGGAGTGTG
  CTACTGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAGTAGAAGAGCAGCGTGAACAG
  GAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCATCCTGTCTCGCCGTATTGCT
  GTTGAATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001024936
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024936.1, NP_001020107.1
• RefSeq Size: 2987 bp
• RefSeq ORF: 1680 bp
• Locus ID: 8936
• Cytogenetics: 6q21
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) lacks two exons in the 5' UTR, as compared to variant 1. Variants 1-4 encode the same protein.