AP4E1 (NM_007347) Human Tagged ORF Clone

CAT#: RC217100

AP4E1 (Myc-DDK-tagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP4E1
• Synonyms: CPSQ4; SPG51; STUT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217100 representing NM_007347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACATAGTGGAGAAGACGCTGACGGCGCTGCCGGGACTCTTTCTGCAGAACCAGCCCGGTGGTG
  GGCCCGCGGCCGCCAAGGCGTCCTTCTCCTCGAGGCTGGGCAGCCTTGTCCGCGGCATCACAGCCCTCAC
  CTCCAAGCACGAAGAAGAAAAATTAATCCAGCAGGAACTGAGTAGTCTGAAAGCGACTGTTTCTGCTCCT
  ACTACAACACTGAAAATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATG
  CTTCCTTTGGCTATATTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTA
  TTTGGCTGTTTCCTTATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAG
  GATCTGCAGAGCACTAACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATTTTCCCCTGCG
  AAATGATTCCAGCTGTTCTTCCATTAATAGAAGATAAACTTCAACATTCTAAGGAGATTGTACGAAGAAA
  AGCTGTTCTGGCATTATACAAATTCCATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTT
  CGGAAAGCACTTTGTGACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTA
  AGGAGAATTCATCTGGATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGG
  AAAGCTCCCAGTAGAATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATA
  CTGGGACTTCTAGGAAAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTAC
  GAAGAGCTGAGTTAAATCACAATGTCACATATGCTATTTTGTTTGAATGTGTGCATACAGTCTATTCTAT
  TTATCCTAAATCGGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATA
  AATCTAAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAAC
  ACCAGATGACAATAATTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCT
  TTACAGAATTACTAATGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGC
  AAAGAAGAGTATGTCATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATATGCTCCTGATA
  ATGCATGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTAGGAGGAGATGTAATGCATCCTGATATTCC
  CAATAACTTTCTGAGACTACTAGCGGAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTCTAT
  GCAGTTCAGTCTTATCTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTA
  TGAGTTGGGTATTAGGGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCTAAGCT
  CTACAAGTTACTTATGAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTGACCAAA
  TTGACATCTCAGGCGCACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATATCTTTGGATA
  CTTGTATGAGACAACATGCATTTGAATTAAAACATTTGCATGAGAATGTGGAACTTATGAAGAGCTTGCT
  TCCAGTTGACAGGAGTTGTGAAGACTTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCT
  GAAGGACTCAGTCAGGGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGG
  AAAAAGTTCTCAATTTTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAGTCTCC
  TGCTGGCATTTCTCTTGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAAGAGACAAATAGC
  TTGAAGCTGGAAGGTATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAGGAAAGCAAAACTG
  GTGATGAAAGTGGAGCTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAATGTAGATCAAGCTATAACTAA
  AAAGGATCAATCTCAAGTTCTTACCCAATCTAAAGAGGAGAAAGAAAAGCAGCTGCTGGCATCATCATTA
  TTTGTTGGTCTAGGATCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCA
  GAAGGAAATCAAAAGTCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGTTCTTC
  CTTTAGTTCTTTGTCAAATGTGGCATATGAAGATGATTATTATTCGAATACTTTGCACGATACAGGAGAC
  AAGGAATTAAAGAAATTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCACTCACAGAACTGCCCTTGG
  TTGAGAAATTCTCATATTGTAGTCTGTCTACACCTTCATTGTTTGCTAATAACAACATGGAAATTTTTCA
  CCCTCCTCAATCTACTGCAGCCTCAGTTGCCAAGGAAAGCTCTTTAGCTTCATCTTTTTTGGAAGAAACT
  ACTGAATACATACACTCAAATGCTATGGAAGTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAA
  TTTGGAAAGATGATTGTTTATTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAAAGTGC
  TGACTTAGAAATTTTTCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGCTGTTTGCCTGTAATG
  GAAGCAGAAAGCACCAAAAGCTTTCAATATAGTGTGCAGATAGAAAAACCTTTTACAGAAGGAAATCTTA
  CTGGTTTTATTAGTTATCATATGATGGATACTCATTCTGCTCAGCTGGAATTTTCTGTAAACTTATCACT
  ATTAGATTTCATTAGACCATTAAAAATCTCAAGTGACGACTTTGGGAAACTCTGGTTATCCTTCGCAAAT
  GATGTGAAACAAAATGTAAAAATGTCAGAATCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAAC
  AGAAACTAAGACTCCATATTATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTCCCATC
  CATCCCCTGCTTACTGCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTCAGATCCTCCTGTTCT
  ACTCTTCCTGACTATTTACTGTATCAGTGTCAAAAGGTGATGGAGGGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217100 representing NM_007347
  Red=Cloning site Green=Tags(s)
  
  MSDIVEKTLTALPGLFLQNQPGGGPAAAKASFSSRLGSLVRGITALTSKHEEEKLIQQELSSLKATVSAP
  TTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVK
  DLQSTNLVEVCMALTVVSQIFPCEMIPAVLPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQVQHIHIKF
  RKALCDRDVGVMAASLHIYLRMIKENSSGYKDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQLLRI
  LGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKI
  NLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQS
  KEEYVIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLY
  AVQSYLTLLDMENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVIAKLYKLLMNDSVSSETKAWLIAAVTK
  LTSQAHSSNTVERLIHEFTISLDTCMRQHAFELKHLHENVELMKSLLPVDRSCEDLVVDASLSFLDGFVA
  EGLSQGAAPYKPPHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDVSGNSAETGLKETNS
  LKLEGIKKLWGKEGYLPKKESKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSL
  FVGLGSESTINLLGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGD
  KELKKFSLTSELLDSESLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKESSLASSFLEET
  TEYIHSNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVTEQPGCCLPVM
  EAESTKSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDFIRPLKISSDDFGKLWLSFAN
  DVKQNVKMSESQAALPSALKTLQQKLRLHIIEIIGNEGLLACQLLPSIPCLLHCRVHADVLALWFRSSCS
  TLPDYLLYQCQKVMEGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007347
• ORF Size: 3411 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007347.1, NM_007347.2, NM_007347.3, NM_007347.4, NP_031373.2
• RefSeq Size: 6768
• RefSeq ORF: 3414
• Locus ID: 23431
• Protein Pathways: Lysosome
• MW: 127.29 kDa
• Gene Summary: This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]