RNF13 (NM_007282) Human Tagged ORF Clone

CAT#: RC217183

RNF13 (Myc-DDK-tagged)-Human ring finger protein 13 (RNF13), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY405260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222407 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF13
• Synonyms: EIEE73; RZF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217183 representing NM_007282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCTTGACTGTCCAGCTCT
  TTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAACTTTGAAAATGCATCTCAGAC
  ATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCAGCTGAAGGTTTAAAGGGTTTTTTGATTAAC
  TCAAAACCAGAGAATGCCTGTGAACCCATAGTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCA
  TCGTGTTAATTAGAAGACTTGATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAA
  GGCAGCCATAGTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
  AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGATGAATTCACAT
  ATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTGGAATACTACCTAATTCCCTT
  CCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATTTTCATGATCACAAAATTTGTCCAGGATAGA
  CATAGAGCTAGAAGAAACAGACTTCGTAAAGATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAG
  GAGATGAGTATGATGTATGTGCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCC
  CTGTTCCCATGCTTATCATTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
  TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAAGAAGAAAATG
  AAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCCCAGTCATTTGGGGCTTTATC
  GGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGACTATGAGGAAGACGACAATGAAGATACTGAC
  AGTAGTGATGCAGAAAATGAAATTAATGAACATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGG
  ATTACAACATAGCAAATACTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217183 representing NM_007282
  Red=Cloning site Green=Tags(s)
  
  MLLSIGMLMLSATQVYTILTVQLFAFLNLLPVEADILAYNFENASQTFDDLPARFGYRLPAEGLKGFLIN
  SKPENACEPIVPPPVKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIEVL
  KKIDIPSVFIGESSANSLKDEFTYEKGGHLILVPEFSLPLEYYLIPFLIIVGICLILIVIFMITKFVQDR
  HRARRNRLRKDQLKKLPVHKFKKGDEYDVCAICLDEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPV
  CKQKVVPSQGDSDSDTDSSQEENEVTEHTPLLRPLASVSAQSFGALSESRSHQNMTESSDYEEDDNEDTD
  SSDAENEINEHDVVVQLQPNGERDYNIANTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007282
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007282.1, NM_007282.2, NM_007282.3, NM_007282.4, NP_009213.1
• RefSeq Size: 2956 bp
• RefSeq ORF: 1146 bp
• Locus ID: 11342
• Domains: RING, PA
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 42.6 kDa
• Gene Summary: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. The specific function of this gene has not yet been determined. Alternatively spliced transcript variants that encode the same protein have been reported. A pseudogene, which is also located on chromosome 3, has been defined for this gene. [provided by RefSeq, Jul 2008]