ARHGAP25 (NM_014882) Human Tagged ORF Clone

CAT#: RC217414

ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY402385) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217414 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ARHGAP25 protein (Cat# TP317414). The protein was produced from HEK293T cells transfected with ARHGAP25 cDNA clone (Cat# RC217414) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP25
• Synonyms: HEL-S-308; KAIA0053
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217414 representing NM_014882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATGACTGGCGAGC
  AGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCCATCAAGATGGGCTGGCTGAA
  GAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTGCTGAGGGCGCAGCAGCTCTACTAC
  TACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCG
  CCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCAT
  GGGACAGGACTCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGG
  AGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTATGAACAGA
  AATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTCATCCTGGAGCACGGCCGGAA
  TGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTGAAGCAGCTGAGAGACGCTTTTGATGCT
  GGGGAGCGGCCCTCCTTTGACAGAGACACAGATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCC
  GAGACCTCCCAGAGCCCGTGGTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAA
  TGCGGATGAGGCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGT
  CTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATGAGTGTGG
  ACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGACCCTGCCGTGATCATGAG
  AGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGAGACCATGAAGTCCTCTTCCCCAAGTCC
  AAGGATATACCCCTGTCACCCCCTGCCCAGAAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTG
  TAGGCTGGGATGCCACTGAAGACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTC
  TGATACAACCAGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGG
  GAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAATGTTTCT
  TGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAATGAATTCTGGTCGCCTTC
  CTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGACTTGCGCCAACTTTCTGACTCC
  CAACGGACTTCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCT
  CTTCCCAAGCCTGTGACTCCAAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAA
  GAACTCTGGAGAAGAGGAAATTGATTCTTTGCAGAGGACGGTCCAAGAGCTACGAAAGGAAATAGAAACA
  CAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCTAAAGTGG
  TGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAGATCAGCCTCCGCAACAT
  GGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAAGAAGTCAAGGAATTTGTCAAA
  TCCATGAAGGAACCCAAGACCGAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217414 representing NM_014882
  Red=Cloning site Green=Tags(s)
  
  MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVLRAQQLYY
  YKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLR
  RVAGTPCGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDA
  GERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYS
  LLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKS
  KDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPR
  EKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQDLRQLSDS
  QRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQRTVQELRKEIET
  QKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRNMERSREDVEKRNKALEEEVKEFVK
  SMKEPKTEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014882
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014882.1, NM_014882.2, NP_055697.1
• RefSeq Size: 2957 bp
• RefSeq ORF: 1917 bp
• Locus ID: 9938
• Domains: RhoGAP, PH
• MW: 72.3 kDa
• Gene Summary: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM, Mar 2008]