BRMS1 (NM_001024957) Human Tagged ORF Clone
CAT#: RC217422
BRMS1 (Myc-DDK-tagged)-Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 2
"NM_001024957" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BRMS1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217422 representing NM_001024957
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCCAGCCTCCAAGCAAAGACACAGAAGAGATGGAAGCAGAGGGTGATTCTGCTGCTGAGATGA ATGGGGAGGAGGAAGAGAGTGAGGAGGAGCGGAGCGGCAGCCAGACAGAGTCAGAAGAGGAGAGCTCCGA GATGGATGATGAGGACTATGAGCGACGCCGCAGCGAGTGTGTCAGTGAGATGCTGGACCTAGAGAAGCAG TTCTCGGAGCTAAAGGAGAAGTTGTTCAGGGAACGACTGAGTCAGCTGCGGTTGCGGCTGGAGGAAGTGG GGGCTGAGAGAGCCCCTGAATACACGGAGCCCCTTGGGGGGCTGCAGCGGAGCCTCAAGATTCGCATTCA GGTGGCAGGGATCTACAAGGGCTTCTGTCTGGATGTGATCAGGAATAAGTACGAATGTGAGCTGCAGGGA GCCAAACAGCACCTGGAGAGTGAGAAGCTGCTGCTCTATGACACGCTGCAGGGGGAGCTGCAGGAGCGGA TCCAGAGGCTGGAGGAGGACCGCCAGAGCCTGGACCTCAGCTCTGAATGGTGGGATGACAAACTGCACGC CAGAGGCAGCTCCAGGTCTTGGGACTCCCTGCCGCCCAGCAAGAGGAAGAAGGCACCTCTGGTTTCTGGC CCATACATCGTGTACATGCTTCAAGAGATCGACATCCTGGAGGACTGGACAGCCATCAAAAAGGCTAGGG CAGCTGTGTCCCCTCAGAAGAGAAAATCGGATGACAGGCGGACCCACAGGCCCCTCAGGGTCTGCCCAGC CAGGCTCCTGTGGTGCTGCTGGGCCCTCCCACTCCATCTGGCACTGGCCTGGACTCCTCCTCTGCCCTCC TCGAGGCCTGCACAGCTGTGGCCGTGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217422 representing NM_001024957
Red=Cloning site Green=Tags(s) MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQ FSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQG AKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSG PYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDDRRTHRPLRVCPARLLWCCWALPLHLALAWTPPLPS SRPAQLWPWS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001024957 |
ORF Size | 870 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024957.1, NP_001020128.1 |
RefSeq Size | 1360 bp |
RefSeq ORF | 873 bp |
Locus ID | 25855 |
Cytogenetics | 11q13.2 |
Protein Families | Druggable Genome |
MW | 33.5 kDa |
Gene Summary | This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302327 | BRMS1 (untagged)-Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 2 |
USD 660.00 |
|
RG217422 | BRMS1 (GFP-tagged) - Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 2 |
USD 460.00 |
|
RC217422L3 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC217422L4 | Lenti ORF clone of Human breast cancer metastasis suppressor 1 (BRMS1), transcript variant 2, mGFP tagged |
USD 620.00 |
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