GPLD1 (NM_001503) Human Tagged ORF Clone

CAT#: RC217578

GPLD1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419906) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217578 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GPLD1 protein (Cat# TP317578). The protein was produced from HEK293T cells transfected with GPLD1 cDNA clone (Cat# RC217578) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPLD1
• Synonyms: GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217578 representing NM_001503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTTTCAGGTTGTGGCCTGGCCTGCTGATCATGTTGGGTTCTCTCTGCCATAGAGGTTCACCGT
  GTGGCCTTTCAACACACGTAGAAATAGGACACAGAGCTCTGGAGTTTCTTCAGCTTCACAATGGGCGTGT
  TAACTACAGAGAGCTGTTACTAGAACACCAGGATGCGTATCAGGCTGGAATCGTGTTTCCTGATTGTTTT
  TACCCTAGCATCTGCAAAGGAGGAAAATTCCATGATGTGTCTGAGAGCACTCACTGGACTCCGTTTCTTA
  ATGCAAGCGTTCATTATATCCGAGAGAACTATCCCCTTCCCTGGGAGAAGGACACAGAGAAACTGGTAGC
  TTTCTTGTTTGGAATTACTTCTCACATGGCGGCAGATGTCAGCTGGCATAGTCTGGGCCTTGAACAAGGA
  TTCCTTAGGACCATGGGAGCTATTGATTTTCACGGCTCCTATTCAGAGGCTCATTCGGCTGGTGATTTTG
  GAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTACCTTGCACGACGCTGGTATGTGCCAGTCAA
  AGATCTACTGGGAATTTATGAGAAACTGTATGGTCGAAAAGTCATCACCGAAAATGTAATCGTTGATTGT
  TCACATATCCAGTTCTTAGAAATGTATGGTGAGATGCTAGCTGTTTCCAAGTTATATCCCACTTACTCTA
  CAAAGTCCCCGTTTTTGGTGGAACAATTCCAAGAGTATTTTCTTGGAGGACTGGATGATATGGCATTTTG
  GTCCACTAATATTTACCATCTAACAAGCTTCATGTTGGAGAATGGGACCAGTGACTGCAACCTGCCTGAG
  AACCCTCTGTTCATTGCATGTGGCGGCCAGCAAAACCACACCCAGGGCTCAAAAATGCAGAAAAATGATT
  TTCACAGAAATTTGACTACATCCCTAACTGAAAGTGTTGACAGGAATATAAACTATACTGAAAGAGGAGT
  GTTCTTTAGTGTAAATTCCTGGACCCCGGATTCCATGTCCTTTATCTACAAGGCTTTGGAAAGGAACATA
  AGGACAATGTTCATAGGTGGCTCTCAGTTGTCACAAAAGCACGTCTCCAGCCCCTTAGCATCTTACTTCT
  TGTCATTTCCTTATGCGAGGCTTGGCTGGGCAATGACCTCAGCTGACCTCAACCAGGATGGGCACGGTGA
  CCTCGTGGTGGGCGCACCAGGCTACAGCCGCCCCGGCCACATCCACATCGGGCGCGTGTACCTCATCTAC
  GGCAATGACCTGGGCCTGCCACCTGTTGACCTGGACCTGGACAAGGAGGCCCACAGGATCCTTGAAGGCT
  TCCAGCCCTCAGGTCGGTTTGGCTCGGCCTTGGCTGTGTTGGACTTTAACGTGGACGGCGTGCCTGACCT
  GGCCGTGGGAGCTCCCTCGGTGGGCTCCGAGCAGCTCACCTACAAAGGTGCCGTGTATGTCTACTTTGGT
  TCCAAACAAGGAGGAATGTCTTCTTCCCCTAACATCACCATTTCTTGCCAGGACATCTACTGTAACTTGG
  GCTGGACTCTCTTGGCTGCAGATGTGAATGGAGACAGTGAACCCGATCTGGTCATCGGCTCCCCTTTTGC
  ACCAGGTGGAGGGAAGCAGAAGGGAATTGTGGCTGCGTTTTATTCTGGCCCCAGCCTGAGCGACAAAGAA
  AAACTGAACGTGGAGGCAGCCAACTGGACGGTGAGAGGCGAGGAAGACTTCTCCTGGTTTGGATATTCCC
  TTCACGGTGTCACTGTGGACAACAGAACCTTGCTGTTGGTTGGGAGCCCGACCTGGAAGAATGCCAGCAG
  GCTGGGCCATTTGTTACACATCCGAGATGAGAAAAAGAGCCTTGGGAGGGTGTATGGCTACTTCCCACCA
  AACGGCCAAAGCTGGTTTACCATTTCTGGAGACAAGGCAATGGGGAAACTGGGTACTTCCCTTTCCAGTG
  GCCACGTACTGATGAATGGGACTCTGAAACAAGTGCTGCTGGTTGGAGCCCCTACGTACGATGACGTGTC
  TAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGCCACTCGCATGTACGCACTCATATCTGAC
  GCGCAGCCTCTGCTGCTCAGCACCTTCAGCGGAGACCGCCGCTTCTCCCGATTTGGTGGCGTTCTGCACT
  TGAGTGACCTGGATGATGATGGCTTAGATGAAATCATCATGGCAGCCCCCCTGAGGATAGCAGATGTAAC
  CTCTGGACTGATTGGGGGAGAAGACGGCCGAGTATATGTATATAATGGCAAAGAGACCACCCTTGGTGAC
  ATGACTGGCAAATGCAAATCATGGATAACTCCATGTCCAGAAGAAAAGGCCCAATATGTATTGATTTCTC
  CTGAAGCCAGCTCAAGGTTTGGGAGCTCCCTCATCACCGTGAGGTCCAAGGCAAAGAACCAAGTCGTCAT
  TGCTGCTGGAAGGAGTTCTTTGGGAGCCCGACTCTCCGGGGCACTTCACGTCTATAGCCTTGGCTCAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217578 representing NM_001503
  Red=Cloning site Green=Tags(s)
  
  MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCF
  YPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQG
  FLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDC
  SHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPE
  NPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNI
  RTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIY
  GNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFG
  SKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKE
  KLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPP
  NGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALISD
  AQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGD
  MTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001503
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001503.2, NM_001503.3, NP_001494.2
• RefSeq Size: 3489 bp
• RefSeq ORF: 2523 bp
• Locus ID: 2822
• Cytogenetics: 6p22.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• MW: 92.35 kDa
• Gene Summary: 'Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]'