GRIA4 (NM_000829) Human Tagged ORF Clone

CAT#: RC217808

GRIA4 (Myc-DDK-tagged)-Human glutamate receptor, ionotrophic, AMPA 4 (GRIA4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIA4
• Synonyms: GluA4; GLUR4; GLUR4C; GLURD; NEDSGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217808 representing NM_000829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATTATTTCCAGACAGATTGTCTTGTTATTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
  CGAGCAGCGTGCAAATAGGTGGTCTCTTCATCCGAAACACAGATCAGGAATACACTGCTTTTCGATTAGC
  AATTTTTCTTCATAACACCAGCCCCAATGCGTCGGAAGCTCCTTTTAATTTGGTACCTCATGTGGACAAC
  ATTGAGACAGCCAACAGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTATTTGCCA
  TTTTTGGACTCTATGATAAGAGGTCGGTACATACCTTGACCTCATTCTGCAGCGCCTTACATATCTCCCT
  CATCACACCAAGTTTCCCTACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAGACCTTCGTTACGAGGA
  GCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGTCTTCCTGTATGACACAGACAGGGGAT
  ACTCGATACTCCAAGCTATTATGGAAAAAGCAGGACAAAATGGTTGGCATGTCAGCGCTATATGTGTGGA
  AAATTTTAATGATGTCAGCTATAGGCAACTTCTAGAAGAACTTGACAGAAGACAAGAGAAGAAGTTTGTA
  ATAGACTGTGAGATAGAGAGACTTCAAAACATATTAGAACAGATTGTAAGTGTTGGAAAGCATGTTAAAG
  GCTACCATTATATCATTGCAAACTTGGGATTCAAGGATATTTCTCTTGAGAGGTTTATACATGGTGGAGC
  CAATGTTACTGGATTCCAGTTGGTGGATTTTAATACACCTATGGTAATCAAACTAATGGATCGCTGGAAG
  AAACTAGATCAGAGAGAGTATCCAGGATCTGAGACTCCTCCAAAGTACACCTCTGCTCTGACTTATGATG
  GAGTCCTTGTGATGGCTGAAACTTTCCGAAGTCTTAGGAGGCAGAAAATTGATATCTCAAGGAGAGGAAA
  TGCTGGGGATTGTCTGGCAAATCCTGCTGCTCCATGGGGCCAGGGAATTGACATGGAGAGGACACTCAAA
  CAGGTTCGAATTCAAGGGCTGACAGGGAATGTTCAGTTTGACCACTATGGACGTAGAGTCAATTACACAA
  TGGATGTGTTTGAGCTGAAAAGCACAGGACCTAGAAAGGTTGGTTACTGGAATGATATGGATAAGTTAGT
  CTTGATTCAAGATGTACCAACTCTTGGCAATGACACAGCTGCTATTGAGAACAGAACAGTGGTTGTAACC
  ACAATTATGGAATCCCCATATGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTATG
  AAGGATACTGTGTAGATTTGGCATCTGAAATTGCAAAACATATTGGTATCAAGTATAAAATTGCCATTGT
  CCCTGATGGAAAATATGGAGCAAGGGATGCAGACACAAAAATCTGGAATGGGATGGTAGGAGAACTTGTT
  TATGGGAAAGCAGAGATTGCTATTGCCCCTCTGACAATCACTTTGGTACGAGAGGAGGTCATTGACTTTT
  CTAAGCCCTTCATGAGTTTGGGCATATCTATCATGATCAAAAAGCCTCAGAAATCCAAACCAGGAGTGTT
  TTCCTTCTTGGATCCTCTGGCCTATGAGATTTGGATGTGCATAGTCTTTGCCTACATTGGTGTCAGCGTG
  GTCTTATTCCTAGTTAGTAGATTTAGTCCATATGAGTGGCACACAGAAGAGCCAGAGGACGGAAAGGAAG
  GACCCAGCGACCAGCCTCCCAATGAGTTTGGCATCTTTAACAGCCTCTGGTTTTCCCTGGGTGCTTTTAT
  GCAGCAAGGATGTGACATTTCACCCAGATCCCTCTCAGGTCGAATTGTTGGAGGTGTTTGGTGGTTCTTT
  ACACTCATCATTATATCATCTTATACTGCTAACCTCGCTGCTTTCCTGACGGTTGAGCGAATGGTCTCTC
  CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTGGATTCAGGATCAAC
  AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTGTATGAAAAGATGTGGACCTACATGCGATCAGCAGAG
  CCATCAGTATTCACTAGGACTACAGCTGAGGGAGTAGCTCGTGTCCGCAAATCCAAGGGCAAATTTGCCT
  TTCTCCTGGAGTCCACTATGAATGAATACATTGAGCAGCGAAAGCCATGTGACACGATGAAAGTGGGAGG
  AAATCTGGATTCCAAAGGCTATGGAGTAGCAACGCCCAAGGGTTCCTCATTAGGAACTCCTGTAAACCTT
  GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG
  AATGTGGACCCAAGGACTCTGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAATGTAGCAGGCGT
  CTTCTACATTCTGGTTGGCGGCTTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC
  AGGGCAGAAGCGAAGAGAATGAAGCTGACCTTTTCTGAAGCCATAAGAAACAAAGCCAGATTATCCATCA
  CTGGGAGTGTGGGAGAGAATGGCCGCGTCTTGACGCCTGACTGCCCAAAGGCTGTACACACTGGAACTGC
  AATCAGACAAAGTTCAGGATTGGCTGTCATTGCATCGGACCTACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217808 representing NM_000829
  Red=Cloning site Green=Tags(s)
  
  MRIISRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDN
  IETANSFAVTNAFCSQYSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRG
  ALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFV
  IDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVIKLMDRWK
  KLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK
  QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQDVPTLGNDTAAIENRTVVVT
  TIMESPYVMYKKNHEMFEGNDKYEGYCVDLASEIAKHIGIKYKIAIVPDGKYGARDADTKIWNGMVGELV
  YGKAEIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSV
  VLFLVSRFSPYEWHTEEPEDGKEGPSDQPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFF
  TLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWTYMRSAE
  PSVFTRTTAEGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSSLGTPVNL
  AVLKLSEAGVLDKLKNKWWYDKGECGPKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKS
  RAEAKRMKLTFSEAIRNKARLSITGSVGENGRVLTPDCPKAVHTGTAIRQSSGLAVIASDLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000829
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000829.1, NM_000829.2, NM_000829.3, NP_000820.1, NP_000820.3
• RefSeq Size: 5508 bp
• RefSeq ORF: 2709 bp
• Locus ID: 2893
• Cytogenetics: 11q22.3
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 100.8 kDa
• Gene Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]'