GRIA4 (NM_000829) Human Untagged Clone

CAT#: SC119639

GRIA4 (untagged)-Human glutamate receptor, ionotrophic, AMPA 4 (GRIA4), transcript variant 1


  "NM_000829" in other vectors (4)

Reconstitution Protocol

USD 1,830.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GRIA4
Synonyms GluA4; GLUR4; GLUR4C; GLURD; NEDSGA
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_000829, the custom clone sequence may differ by one or more nucleotides


ATGAGGATTATTTCCAGACAGATTGTCTTGTTATTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
CGAGCAGCGTGCAAATAGGTGGTCTCTTCATCCGAAACACAGATCAGGAATACACTGCTTTTCGATTAGC
AATTTTTCTTCATAACACCAGCCCCAATGCGTCGGAAGCTCCTTTTAATTTGGTACCTCATGTGGACAAC
ATTGAGACAGCCAACAGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTATTTGCCA
TTTTTGGACTCTATGATAAGAGGTCGGTACATACCTTGACCTCATTCTGCAGCGCCTTACATATCTCCCT
CATCACACCAAGTTTCCCTACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAGACCTTCGTTACGAGGA
GCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGTCTTCCTGTATGACACAGACAGGGGAT
ACTCGATACTCCAAGCTATTATGGAAAAAGCAGGACAAAATGGTTGGCATGTCAGCGCTATATGTGTGGA
AAATTTTAATGATGTCAGCTATAGGCAACTTCTAGAAGAACTTGACAGAAGACAAGAGAAGAAGTTTGTA
ATAGACTGTGAGATAGAGAGACTTCAAAACATATTAGAACAGATTGTAAGTGTTGGAAAGCATGTTAAAG
GCTACCATTATATCATTGCAAACTTGGGATTCAAGGATATTTCTCTTGAGAGGTTTATACATGGTGGAGC
CAATGTTACTGGATTCCAGTTGGTGGATTTTAATACACCTATGGTAATCAAACTAATGGATCGCTGGAAG
AAACTAGATCAGAGAGAGTATCCAGGATCTGAGACTCCTCCAAAGTACACCTCTGCTCTGACTTATGATG
GAGTCCTTGTGATGGCTGAAACTTTCCGAAGTCTTAGGAGGCAGAAAATTGATATCTCAAGGAGAGGAAA
TGCTGGGGATTGTCTGGCAAATCCTGCTGCTCCATGGGGCCAGGGAATTGACATGGAGAGGACACTCAAA
CAGGTTCGAATTCAAGGGCTGACAGGGAATGTTCAGTTTGACCACTATGGACGTAGAGTCAATTACACAA
TGGATGTGTTTGAGCTGAAAAGCACAGGACCTAGAAAGGTTGGTTACTGGAATGATATGGATAAGTTAGT
CTTGATTCAAGATGTACCAACTCTTGGCAATGACACAGCTGCTATTGAGAACAGAACAGTGGTTGTAACC
ACAATTATGGAATCCCCATATGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTATG
AAGGATACTGTGTAGATTTGGCATCTGAAATTGCAAAACATATTGGTATCAAGTATAAAATTGCCATTGT
CCCTGATGGAAAATATGGAGCAAGGGATGCAGACACAAAAATCTGGAATGGGATGGTAGGAGAACTTGTT
TATGGGAAAGCAGAGATTGCTATTGCCCCTCTGACAATCACTTTGGTACGAGAGGAGGTCATTGACTTTT
CTAAGCCCTTCATGAGTTTGGGCATATCTATCATGATCAAAAAGCCTCAGAAATCCAAACCAGGAGTGTT
TTCCTTCTTGGATCCTCTGGCCTATGAGATTTGGATGTGCATAGTCTTTGCCTACATTGGTGTCAGCGTG
GTCTTATTCCTAGTTAGTAGATTTAGTCCATATGAGTGGCACACAGAAGAGCCAGAGGACGGAAAGGAAG
GACCCAGCGACCAGCCTCCCAATGAGTTTGGCATCTTTAACAGCCTCTGGTTTTCCCTGGGTGCTTTTAT
GCAGCAAGGATGTGACATTTCACCCAGATCCCTCTCAGGTCGAATTGTTGGAGGTGTTTGGTGGTTCTTT
ACACTCATCATTATATCATCTTATACTGCTAACCTCGCTGCTTTCCTGACGGTTGAGCGAATGGTCTCTC
CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTGGATTCAGGATCAAC
AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTGTATGAAAAGATGTGGACCTACATGCGATCAGCAGAG
CCATCAGTATTCACTAGGACTACAGCTGAGGGAGTAGCTCGTGTCCGCAAATCCAAGGGCAAATTTGCCT
TTCTCCTGGAGTCCACTATGAATGAATACATTGAGCAGCGAAAGCCATGTGACACGATGAAAGTGGGAGG
AAATCTGGATTCCAAAGGCTATGGAGTAGCAACGCCCAAGGGTTCCTCATTAGGAACTCCTGTAAACCTT
GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG
AATGTGGACCCAAGGACTCTGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAATGTAGCAGGCGT
CTTCTACATTCTGGTTGGCGGCTTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC
AGGGCAGAAGCGAAGAGAATGAAGCTGACCTTTTCTGAAGCCATAAGAAACAAAGCCAGATTATCCATCA
CTGGGAGTGTGGGAGAGAATGGCCGCGTCTTGACGCCTGACTGCCCAAAGGCTGTACACACTGGAACTGC
AATCAGACAAAGTTCAGGATTGGCTGTCATTGCATCGGACCTACCATAA


>OriGene 5' read for NM_000829 unedited
CGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAACAAAGAGAGCGCGCGCCAGG
NAGAGGAGAAAAGAAGATGAGGATTATTTCCAGACAGATTGTCTTGTTATTTTCTGGATT
TTGGGGACTCGCCATGGGAGCCTTTCCGAGCAGCGTGCAAATAGGTGGTCTCTTCATCCG
AAACACAGATCAGGAATACACTGCTTTTCGATTAGCAATTTTTCTTCATAACACCAGCCC
CAATGCGTCGGAAGCTCCTTTTAATTTGGTACCTCATGTGGACAACATTGAGACAGCCAA
CAGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTATTTGCCATTTT
TGGACTCTATGATAAGAGGTCGGTACATACCTTGACCTCATTCTGCAGCGCCTTACATAT
CTCCCTCATCACACCAAGTTTCCCTACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAG
ACCTTCGTTACGAGGAGCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGT
CTTCCTGTATGACACAGACAGGGGATACTCGATACTCCAAGCTATTATGGAAAAAGCAGG
ACANAATGGTTGGCATGTCAGCGCTATATGTGTGGAAAATTNTAATGATGTCAGCTATAG
GCAACTTCTAGAAGAACTTGACAGAAGACAAGAGAAGAAGTTTGTAATAGACTGTGAGAT
AGAGAGACTTCANACATATTANAACAGATTGTAGTGTTGGAAAGCATGTTTAAGGCTACC
ATTATATCATTGCANACTTGGGATTCAAGGATATTTCTCTGAGAGGTTATACATGGTGGA
GCCAATGGTACTGGGATCCAGTTGGTGGATTTTAAACACCTAGGGAAACCAACTAAGGGA
ACGCTGGAAGAAACTGATCAGAAGAGTTTCCGGATCTGAGACTCCTCCCAGA
>OriGene 3' read for NM_000829 unedited
CCCCCTACTTAGTCCGCGGCCGCAATCTACAGCGGGTTTTTTTTTTTTTTTTTATTGCAA
GAACATCTGCTGTTTTATTCTTTTTGGTGGGTACAAAAACCACAATGTTGTACACAGAAT
GAGATTATAAATAATACCATTAACATGAGTGACCTGTCATCTCTTTCGAGTCTACACCGC
AGACTGATGATTGAACCTAAACGTTTTCTTCCTTTTGCTTTTCTTCCTGGTTGTGAGACA
AATGCAATCGGTGAATCGACTCATGTGACATAGTGAAGGTTACAGCAAACAAGAAGATGA
CCAACAACTTTTATAAAAAAAAGTCTCTAAAGTTTATTACAGTGATGGCATTTATATCAA
TTATTGCAATGTATTGTTCCCTAAGATGGTTAGAAAAAAGAATTGTGTTGTCCTCTACTG
AATTTACGTCAGTTAAAAAGCAAGCATGTTTCAGTATTTGTTTTCTCCAAAGTAAGTGTA
CATACTTCCTTTATTTTTTTTTTCCCACAGGTTTTACATGTCTGCTCATTATTTTCTTAC
CCCACCTACCGCTCCCCCCCCCTCCCTCCCACCATCCCCCCCCCGCCATCCCGACCACCC
CCCCCCCCCTTTCCCTCCTACCTCTCCCACAACCCTCATATTCCCCTCCTCCCCCCCCCC
CCCCCTTCCTCCCCTCCCTTCTCTTCTCCCCCCCCCTCTCCCCCCCCCACCTCCCTCCCA
CCCCCCCCCCCCCCCCCCTCCACCCTCCAACCCTCCCCCCCCTCCCCCCCCCCTCTCTCT
CTTTTCTTTCATCCCCTACCTTCACTACCCCCACACCCCACCTCACTCCCCCCACCCACC
CTACCACATCCCCCACCCCTTCCTTCCTTCCCCCCCCAACTCACCC
Restriction Sites NotI-NotI     
ACCN NM_000829
Insert Size 4300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000829.1, NP_000820.1
RefSeq Size 5508 bp
RefSeq ORF 5508 bp
Locus ID 2893
Cytogenetics 11q22.3
Domains lig_chan, ANF_receptor
Protein Families Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longest isoform (1). RNA editing (AGA->GGA) changes Arg765Gly. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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