ADAM28 (NM_021777) Human Tagged ORF Clone

CAT#: RC217827

ADAM28 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 28 (ADAM28), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY402874) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217827 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM28
• Synonyms: ADAM 28; eMDC II; eMDCII; MDC-L; MDCL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217827 representing NM_021777
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCAAGGTCTCCTGCCAGTCAGTCTCCTCCTCTCTGTTGCAGTAAGTGCTATAAAAGAACTCCCTG
  GGGTGAAGAAGTATGAAGTGGTTTATCCTATAAGACTTCATCCACTGCATAAAAGAGAGGCCAAAGAGCC
  AGAGCAACAGGAACAATTTGAAACTGAATTAAAGTATAAAATGACAATTAATGGAAAAATTGCAGTGCTT
  TATTTGAAAAAAAACAAGAACCTCCTTGCACCAGGCTACACGGAAACATATTATAATTCCACTGGAAAGG
  AGATCACCACAAGCCCACAAATTATGGATGATTGTTATTATCAAGGACATATTCTTAATGAAAAGGTTTC
  TGACGCTAGCATCAGCACATGTAGGGGTCTAAGGGGCTACTTCAGTCAGGGGGATCAAAGATACTTTATT
  GAACCTTTAAGCCCCATACATCGGGATGGACAGGAGCATGCACTCTTCAAGTATAACCCTGATGAAAAGA
  ATTATGACAGCACCTGTGGGATGGATGGTGTGTTGTGGGCCCACGATTTGCAGCAGAACATTGCCCTACC
  TGCCACCAAACTAGTAAAATTGAAAGACAGGAAGGTTCAGGAACATGAGAAATACATAGAATATTATTTG
  GTCCTGGATAATGGTGAGTTTAAAAGGTACAATGAGAATCAAGATGAGATCAGAAAGAGGGTATTTGAGA
  TGGCTAATTATGTCAACATGCTTTATAAAAAGCTCAATACTCATGTGGCCTTAGTTGGTATGGAAATCTG
  GACTGACAAGGATAAGATAAAGATAACCCCAAATGCAAGCTTCACCTTGGAGAATTTTTCTAAATGGAGG
  GGGAGTGTTCTCTCAAGAAGAAAGCGTCATGATATTGCTCAGTTAATCACAGCAACAGAACTTGCTGGAA
  CGACTGTGGGTCTTGCATTTATGTCTACAATGTGTTCTCCTTATTCTGTTGGCGTTGTTCAGGACCACAG
  CGATAATCTTCTTAGAGTTGCAGGGACAATGGCACATGAAATGGGCCACAACTTTGGAATGTTTCATGAC
  GACTATTCTTGCAAGTGTCCTTCTACAATATGTGTGATGGACAAAGCACTGAGCTTCTATATACCCACAG
  ACTTCAGTTCCTGCAGCCGTCTCAGCTATGACAAGTTTTTTGAAGATAAATTATCAAATTGCCTCTTTAA
  TGCTCCATTGCCTACAGATATCATATCCACTCCAATTTGTGGGAACCAGTTGGTGGAAATGGGAGAGGAC
  TGTGATTGTGGGACATCTGAGGAATGTACCAATATTTGCTGTGATGCTAAGACATGTAAAATCAAAGCAA
  CTTTTCAATGTGCATTAGGAGAATGTTGTGAAAAATGCCAATTTAAAAAGGCTGGGATGGTGTGCAGACC
  AGCAAAAGATGAGTGCGACCTGCCTGAAATGTGTAATGGTAAATCTGGTAATTGTCCTGATGATAGATTC
  CAAGTCAATGGCTTCCCTTGCCATCACGGGAAGGGCCACTGCTTGATGGGGACATGCCCCACACTGCAGG
  AGCAGTGCACAGAGCTGTGGGGACCAGGTAGGAGGACAAATCCTTTCCCCTGTGCATGTGCGAAGGAAAA
  TCATTTCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217827 representing NM_021777
  Red=Cloning site Green=Tags(s)
  
  MLQGLLPVSLLLSVAVSAIKELPGVKKYEVVYPIRLHPLHKREAKEPEQQEQFETELKYKMTINGKIAVL
  YLKKNKNLLAPGYTETYYNSTGKEITTSPQIMDDCYYQGHILNEKVSDASISTCRGLRGYFSQGDQRYFI
  EPLSPIHRDGQEHALFKYNPDEKNYDSTCGMDGVLWAHDLQQNIALPATKLVKLKDRKVQEHEKYIEYYL
  VLDNGEFKRYNENQDEIRKRVFEMANYVNMLYKKLNTHVALVGMEIWTDKDKIKITPNASFTLENFSKWR
  GSVLSRRKRHDIAQLITATELAGTTVGLAFMSTMCSPYSVGVVQDHSDNLLRVAGTMAHEMGHNFGMFHD
  DYSCKCPSTICVMDKALSFYIPTDFSSCSRLSYDKFFEDKLSNCLFNAPLPTDIISTPICGNQLVEMGED
  CDCGTSEECTNICCDAKTCKIKATFQCALGECCEKCQFKKAGMVCRPAKDECDLPEMCNGKSGNCPDDRF
  QVNGFPCHHGKGHCLMGTCPTLQEQCTELWGPGRRTNPFPCACAKENHFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021777
• ORF Size: 1620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021777.1, NM_021777.2, NM_021777.3, NM_021777.4, NP_068547.2
• RefSeq Size: 2076 bp
• RefSeq ORF: 1623 bp
• Locus ID: 10863
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• MW: 61.1 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]